Vikas Sonakya, PhD Email: acd447@r.postjobfree.com

Ph: 201-***-****

SUMMARY

Solid experience in Molecular biology, cell Biology, signal transduction,

cancer and other complex diseases. I am an energetic team coordinator with

strong communication, interpersonal, multi-tasking, and collaborating

management skills. Ability to effectively communicate and established

strategies plans with other research groups. Conceived and prepared

protocol, and its implementation to get expected result.

TECHNICAL SKILLS (in brief)

Scientific: Mammalian Cell and tissue culture, Gene silencing / RNAi /

shRNA (Knockdown) Technology, SNP Analysis, cDNA microarray, cDNA Library

construction, whole genome DNA sequencing, Data analysis, Gene expression,

protein extraction and characterization, PCR, RT-PCR-Taqman probe, DNA/RNA

isolation and characterization, Cloning, Metabolic Engineering FISH

(Fluorescence in-situ Hybridization using 16sRNA probes), Confocal laser

Scanning Microscopy, Northern Blotting, Southern Blotting, Western

Blotting, Radioactive labelling (32P), Luciferase, LacZ, GFP reporter

genes, Signal Transduction, Gene target, Mapping, PAGE, SDS PAGE, DGGE,

Transfection, Transformation, Antibiotic testing assay, Mutation (Site

specific). Expertise in Molecular biology, Microbiology, System Biology.

Instruments:

CLSM (Confocal laser Scanning Microscopy)/ light and fluorescent

microscopy, Anaerobic cell culture chamber, Nano drops, Phosphorimager,

Fluorescence Microscopy, HPLC, ELISA reader, PCR/RT-PCR, Chromatography

(Gas, Liquid), scintillation counter, Gel electrophoresis.

Computational: Ingenuity Pathway Analysis (IPA, Ingenuity Systems), Drug

Target, Molecular toxicology analysis, Metabolomics, Genome studio

(Illumina, Inc.), Genome workbench (NIH/NCBI), Genotyping console

(Affymetrix), Gene Cluster, SNP and microarray software tools, Vector NTI,

NCBI data mining tools, GWAS data mining tools, dbGAP, Plink, iGrafx (six

sigma), Basics SQL, perl, Splus,birdseed, plink, MS office suits 2003/2007.

PROFESSIONAL EXPERIENCE

Mount Sinai Hospital, New York, NY 2013 -

Present

Biologist,

Project: Whole genome, Genotype data analysis of Childhood absence

epilepsy.

> Data Mining from avalilable databases like NCBI, dbSNP.

> Pathway analysis from ingenuity, system biology, gene and protein

interaction.

> Whole genome sequencing. Data formatting and analysis using MuStat.

> Paper publication.

Rockefeller University, New York, NY 2009 -

2013

Biologist,

Project: "Genome Wide Analysis Study (GWAS): Whole genome, Genotype data

analysis of complex diseases", like Psoriasis, Melanoma, Multiple

sclerosis, Fibromyalgia, Narcotic Drug Addiction.

> Single Nucleotide Polymorphism (SNP) array and in house data

organization.

> Pathway analysis, system biology, gene and protein interaction.

> Validation of Genotype calling using different platform like Illumina,

Affymetix, NCBI.

> Whole genome sequencing. Data formatting and analysis using MuStat.

> Data presentation with the result in whole GWAS Manhattan Plot.

> Data handled: Childhood absence epilepsy (CCHMC), Psoriasis (dbgap),

Melanoma (dbgap), Multiple sclerosis, Fibromyalgia (Wellcome Trust center

for Human Research, UK), Narcotic Drug Addiction (RU).

> Establish effectively communicate and strategies plans with other

research groups in different organization to make joint grant proposal

including paper publication.

Center for Study of Gene Structure and Function, CUNY, New York, NY

2004 - 2009

Research Associate II,

Project: "Phospho-protein Phosphatase 2A, Signaling and Development in

Dictyostelium, Using RNA interference technology (RNAi)".

> Gene expression and Signal Transduction pathway exploration.

> Screening RNAi sequence to Knockdown PP2a gene.

> Designing and Implementation of high throughput shRNA, Micro RNA and

siRNA. RNA interference by an inducible Polymerase II, III promoter and

analyze its effect on cell growth and transformation rate.

> Conceived, prepared and follow the amendments in protocol and its

implementation to get expected result.

> Once a year draft and present grant proposal and get its approval for the

committee members. Establish effectively communicate and strategies plans

with other research groups to make joint grant proposal.

> Write and defend scientific publications from the laboratory work and

encourage other colleagues to participate in the lab network.

> Served as an active member of scientific organizing committee in

arranging several national and international meetings and conferences.

> Reviewing scientific articles from outside the department.

> Maintain lab notebook and keep track of the entire inventory.

Division of Biotechnology, Link ping University, Sweden

2003 - 2004

Post Doctoral Fellow

Project: "Studies on Stress responses in CHO cells using Microarray

Technology",

> Implementation of high throughput microarray date for better

understanding of stress in mammalian cells.

> Make gene profile of heat shock protein under the effect of heat and

other stress like acid and oxidative.

> Prepare progress report and present reports regularly in front of

departmental head and bimonthly in front of funding agency.

> Prepare a proposal for extension of the existing project for more grants.

> Publish paper in high impact factor journal.

> Collection of data for reviewing other literature published in different

parts of the world.

> Mentoring Grad and Undergrad students.

Technical University of Munich (TUM), Germany

2001 - 2002

Doctoral Fellow

Project: "Study on Rumen Enhanced Solid Incubation (RESI)" Using Confocal

Laser scanning microscope (CLSM), at Institute for Water Quality and Waste

Management, TUM, Germany. Under Prof. Dr. Ing. Peter A Wilderer.

> Established a novel method to study microbial community structure in

rumen.

> Published papers in various world class journals of the field

> Collaborated with different groups working in the field rRNA probe

design.

> Prepare final progress report and present bimonthly in departmental

seminars.

> Write Patents in the outcome of the cutting edge research.

EDUCATION

PhD Biotechnology, University of Delhi, Institute of July 1997 - Sep 2003

Genomics and Integrative Biology (IGIB), Delhi.

Govt. of INDIA.

M.S. Biosciences, Indian Institute of Technology (IIT) June 1995 - June

Roorkee, Department of Biotechnology, INDIA. 1997

B.S. Biology, H. N. B. University, Dehra Dun, INDIA. June 1991- March1994

CERTIFICATIONS/COURSES

> Clinical Research certificate at Rockefeller University, NY,

> Clinical Research Training and medical education certificate, National

Institute of Health (NIH)

> Extramural Research certificate "Protecting Human Research Participants",

NIH

> HIPPA training from Office of Human Subject Research (OHSR), NIH

> Advance course on Good Clinical Practice (GCPs), NIH

> Incorporating Cancer Clinical Trials Into Your Practice, NIH

> Refresher course for principle investigator (PI) for animal use, NIH

> Corrective and Preventive Action (CAPA) [21CFR 820.100] course, FDA

WRITING AND REVIEWING SKILLS

. Good writing and reviewing skills for peer reviewed publications in

scientific journals. Papers published in Journals: 13 (Provide list

on request)

. Good reviewing skills. Papers Reviewed: 41

. Good Knowledge of protocol and patent writing and Filing. Patents: 6

(Provide list on request)

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