PAYAL KHANNA

917-***-****

accxms@r.postjobfree.com

http://www.linkedin.com/pub/payal-khanna/30/86a/626

OBJECTIVE: To obtain a position as a Biochemist/Molecular Biologist

EDUCATION

Albany College of Pharmacy and Health Sciences, Albany, NY September 2012- May 2014(expected)

Candidate for MS in Pharmaceutical Sciences (Pharmacology track), GPA 3.7

St. John’s University, NY September 2011-August 2012

Completed a year of graduate studies in Pharmacology

Dr. Bhanuben Nanavati College of Pharmacy, India July 2007- May 2011

University of Mumbai, BS in Pharmaceutical Sciences

RELATED EXPERIENCE

Graduate Research Assistant (Molecular biology lab), January 2013- present

Albany College of Pharmacy and Health Sciences, NY

• Performed nucleic acid isolation, purification and quantification

• Assisted my PI in confocal microscopy and protein quantification assays

• Performed cell transfection, gene knockdown techniques and plasmid engineering

• Assisted my PI in his experiments and troubleshooting and designing protocols

• Also helped in maintaining lab supplies inventory and equipment, handling process orders and helping the

undergraduate students which improved my interpersonal and verbal skills

• Documented experimental results, thus developed proficiency with MS Office(Word, Excel, Power Point) and

Adobe Photoshop

• Assisted in writing scientific articles

• Presented experimental results and data at lab meetings which enhanced communication skills

• Proficient in data analysis

St. John’s University, NY May 2012- August 2012

Conducted preliminary studies for Research Project in a Biochemistry lab at St. John’s University which included:

• Mammalian cell culturing sterile techniques, maintenance and characterization of multiple cell lines

• Cell based Cytotoxicity Assays (MTT) on multiple cell lines including Jukart cells,SKMEL-2 cells, HELA cells,

HEK293 cells using Cisplatin and Pateamine analogs

• Handled Jukart cells, MDAMB2 cells, SKMEL-2 cells, HELA cells, HEK293 cells, performed passaging and

splitting of these cell lines

• Preparation and transformation of BL-21 and DH5α competent cells

• Performed RNA isolation using RNase easy Mini kit

• Transformation of HCV cap dependent plasmid into Escherichia Coli (strain DH-5)

PAYAL KHANNA

As an Undergraduate Pharmaceutical Lab Student at University of Mumbai, received training in: July 2007-

May 2011

• Basic Microbiology Techniques- working in aseptic area, handling microorganisms, bacterial cells

• Operated UV Visible Spectrophotometer, photo colorimeter and pH Meter

• Familiar with lab techniques such as Histology, Staining of tissues, titration and Volumetric Assays

RESEARCH PROJECTS

1. MS thesis -“VDUP1-dependent regulation of neuroblast proliferation and progeny differentiation in the Drosophila

CNS”

• Set up various crosses and mutants of the fruit fly Drosophila Melanogaster using gene knockdown and gene

expression techniques

• Dissecting the adult and larval fly brains and performing immunohistochemistry

• Mounting the brains and analyzing using confocal microscope and immunofluorescence

• Quantifying the gene expression or knockdown using western blots and RT-PCR

• Quantifying the expression of proteins by measuring their fluorescence intensity using CellSens Dimension

2. Molecular Cloning-Designed a new plasmid vector construct pUAST VDUP1-GFP for assessing the upregulation

of VDUP1

• Gel purified the plasmid vector using ultra clean gel spin DNA purification kit

• Cut the vector using restriction enzymes and then performed DNA ligation

• Transformation/ transfection of competent Escherichia Coli cells

• Isolated plasmid DNA using plasmid mini prep kit

• Analysis of VDUP1-GFP miniprep DNA

• Running polymerase chain reaction (PCR) to verify the VDUP1-GFP insert

• Analyzed the insert using gel electrophoresis

• The DNA was quantified and sent to Rainbow transgenics for genetic engineering of the vector into the fruit fly

Drosophila embryos thus developing a new mutant fly for further analysis for tumorigenesis

WORK EXPERIENCE

Indoco Remedies, Mumbai May-June 2010

Intern

PAYAL KHANNA

• Formulation and Development Department in Tablet, capsule, oral liquids manufacturing and troubleshooting

• Quality Control and Quality Assurance laboratory

• Design and Manufacture of equipment used in the formulation department

• Analytical department- analyzing samples using HPLC, GC and troubleshooting

POSTER PRESENTATIONS

• “The VDUP1 tumor suppressor gene negatively regulates larval Drosophila central brain growth by controlling

neuroblast proliferation and progeny differentiation.” at the “Neurobiology of Drosophila” conference held in

October 2013 at Cold Spring Harbor Laboratory, NY

• “Sterilization & its novel applications” at the Rx Festival organized by the Indian Pharmaceutical Association(IPA)

(2009)

• “Antidepressants-role of nicotine as an antidepressant” at the UICT- Exergy Tech Fest (2010)

• “Recombinant DNA technology-its future” at NMIMS-Isthmus (2010)

• “Oleoyl Serine- A potentially viable template for treating osteoporosis” at UICT- Exergy Tech Fest (2011)

• “Novel non-invasive drug delivery system for pulmonary tuberculosis” at the Rx Fest (2011)

• “Nanosuspensions- The next Blockbuster” at UICT- Exergy Tech Fest (2011)

ACHIEVEMENTS

Winner at Institute Of Chemical Technology Fest-“Exergy 2011", Mumbai University for paper presentation on “Oleoyl

Serine a Potentially viable drug template for treating Osteoporosis.”

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