Shane O’Brien

*** ******* ****

Collegeville, Pa 19426

accu9o@r.postjobfree.com

610-***-****

Education:

West Chester University of Pennsylvania Millersville University of Pennsylvania

Masters of Science in Biology, 2013 Bachelors of Science in Biology, 2008

Thesis: HSP90 mediates proteolytic activity of ovarian carcinoma Major GPA: 3.28

Major GPA: 4.0

Publications:

Liu, H.*, Xiao, F.*, Serebriiskii, I.G., O’Brien, S.W., Maglaty, M.A., Astsaturov, I., Martin, L.P., Litwin, S., Proia, D.A., Golemis,

E.A. and Connolly, D.C. Network analysis identifies an HSP90-central hub susceptible in ovarian cancer.Clin. Cancer Res. 2013 Jul 30

Do, T.-V., Xiao, F., Bickel, L.E., Klein-Szanto, A.J., Pathak, H.B., Hua, X., Howe, C., O’Brien, S. W., Maglaty, M., Ecsedy, J.A.,

Golemis, E.A., Schilder, R.J., Godwin, A.K., and Connolly, D.C. Aurora kinase A mediates epithelial ovarian cancer migration and

adhesion. Oncogene. 2013 Jan 21

Hensley, H.H., Roder, N.A., O’Brien, S.W., Bickel, L.E., Xiao, F., Litwin S., and Connolly, D.C. 2012. Combined in vivo molecular

and anatomic imaging for detection of ovarian carcinoma-associated protease activity and integrin expression in mice. Neoplasia 14:451-

462

Experience:

Fox Chase Cancer Center, January 2011 – current

Developmental Therapeutics

Technical Specialist

• Work in an academic laboratory focused on ovarian carcinoma and development of therapeutics

• Documentation of experimental methods and results

• Trained incoming graduate and summer students in several laboratory techniques

• Perform in vitro techniques on human and mouse cell lines including:

o 2D and 3D cell culturing

o Cell viability, invasion, and motility assays

o siRNA and luciferase transfection

o Cell imaging – live cell, IF, cell sorting, and near infrared microscopy

• Perform in vivo techniques including:

o Animal husbandry

o Animal imaging – MRI, bioluminescence, fluorescent mediated tomography

o Formulation and administration of drugs to ovarian cancer mouse models

o Development of human orthotopic ovarian cancer and patient derived xenograft mouse models

• Perform lab bench techniques

o DNA, RNA, and protein isolation

o PCR, Western blotting, zymography, immunoprecipitation

o Paraffin and frozen sectioning, IHC, DAKO Autostainer, IF, Laser micro dissection

• Responsible for lab supply ordering and management

o Management of Liquid N2 cell storage

o Monitoring and cleaning of CO2 incubators

o Preparation of shared supplies

Charles River Laboratories, May 2008 – November 2010

Preclinical Services Pennsylvania

Necropsy Technician II

• Worked with GLP regulatory guidelines

• Conducted studies following protocol and standard operating protocols

• Responsible for collection, organization, and accuracy of data

• Prepared necessary paperwork and supplies for a study

• Performed many types of necropsy procedures including:

o Gross necropsy of rats, rabbits, and mice

o Bone marrow smears, differential slides, and reticulocyte slides

o Caesarean sections and gross necropsy of fetuses

• Collected, processed, and shipped blood samples

• Organized and archived tissues

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