Renata L. Smith

*** ********** **.

Morrisville NC 27560

919-***-****

accpkz@r.postjobfree.com

EDUCATION:

Bachelor of Science

May 2000

Rider University

Lawrenceville, NJ

Major: Cellular and Molecular Biology, Chemistry, and Spanish

Physician's Assistant-Haematology

2003

Southern Illinois University

Carbondale, IL

Languages: English, Spanish, Russian

RESEARCH SKILLS:

RT-PCR, qPCR, PCR, ELISA, RIA, Alpha Screen, DNA isolation from whole blood

and bacteria, DNA library construction and screening, DNA gel

electrophoresis and SDS-PAGE, Western Blot, RNA and cDNA isolation,

surgery, venipuncture and postmortem sample collection using murine models,

cytokine assessment using Luminex system (multiplex), gene cloning and

expression, bacterial and mammalian tissue and cell culture, cytology

preparations (Masson's Trichrome, hematoxylin and eosin, Gram stains) with

interpretation, flow cytometry, chromogenic assays (ACL Elite), ST4

coagulometry, Octet kinetics, UFDF, cell assays

SOFTWARE:

Skilled in GraphPad Prism, Vector NTI, BLAST, data tracker, Sigma Plot,

Cricket, Microsoft Excel, PowerPoint, Word, SAP

EXPERIENCE:

Research Technician II

11/2013-current

Duke University-Surgery/Urology

Durham, NC

-Evaluating correlation between obesity and prostate cancer

-Compared western diet versus high fat versus low carb diets in murine

models

-Maintained prostate and breast cancer cell lines for proliferation assays

and xenograft model formation

-Isolated protein and RNA for qPCR analysis

-Supported post docs and lower associates in daily activities

Senior Associate Scientist I

3/2009-10/2012

Grifols, Inc.

RTP, NC

-Created an approved factor 8 immunotherapy assay for Hemophilia and

Christmas

disease patients using ST4 and ACL Elite coagulometers(new product

development)

-Processed and analyzed blood and urine post treatment (CRO environment)

-Extensive experience with in vivo physiological and pharmacological

studies using

murine/rabbit models

-Created factor 8 and vWF ELISAs

-Monitored innate vWF activity and antigen levels with data analysis to

follow

-Performed UFDF on large scale mammalian cell culture supernatants for

technology transfer

-Analyzed blood clotting proteins on Forte Bio Octet using kinetic assays

-Testing, formulation, validation, stability of factor proteins in GMP/GLP

environment

-Aided in phase 3 clinical trials for plasmin product

-Supervised lower associates in daily activities

-Acted as safety officer for preclinical department

Research Analyst I

4/2007-3/2009

Duke University-Pulmonary Transplant

Durham, NC

-Investigation of the connection between adaptive and innate immunity with

regards to pulmonary function in murine models and humans

-Performed bone marrow transplants and adoptive transfers on MHC matched

and mismatched mice

-Interpreted mouse and human cytokine, chemokine production, and mRNA

abundance using the BioPlex Luminex system and ABI 7500s with data

analysis to follow

-Processed and analyzed human bronchoalveolar lavage fluid pre- and post-

pulmonary transplants

-Created a transgenic murine line

-Collaboration with and instruction of pulmonary fellows, medical students,

and undergraduates on their respective projects

Research Specialist in Life Sciences

9/2001-6/2005

University of Illinois, College of Medicine

Urbana, IL

-Evaluated the mammalian blood-clotting cascade to provide opportunities

for the development of diagnostic tools and treatments of human bleeding

disorders

-Developed novel clinical assays (ELISA based) for assessing human blood

clotting components

-Found prevalence of single nucleotide polymorphisms (SNPs) of factor VIIA

in

individuals with thrombotic risk factors with data analysis to follow

-Improved HPLC protein purification by creating gene expression systems

-Supervised and managed undergraduate student research projects

Research Associate

5/2000-6/2001

Orchid Biosciences, Inc.

Princeton, NJ

-Created new kit assays with a focus on stability testing, production, and

quality control

-Developed genotyping system using primer extension that has been adapted

to Luminex

-Created and reviewed SOPs for current research project

-Aseptic production in a GMP/clean room environment

-Acted as a liaison between research and development and quality control

departments

Research Assistant

9/1998-5/2000

Department of Biology, Rider University

Lawreneceville, NJ

-Analyzed B and T cell interactions in murine models with an emphasis on T

cell cytokine and IgM production caused by superantigens

Associate Research Assistant

5-8/1999

Kimeragen, Inc.

Newtown, PA

-Transfected HL-60 cell line with CD4+ protein for chimeraplasty repair

PRESENTATIONS & PUBLICATIONS:

Martinu T, Garantziotis S, Smith R., Kinnier CV, Kelly FL, Snyder LD, Jiang

D, Noble PW, Palmer SM. CXCL10 in murine alloimmune lung injury. American

Thoracic Society Annual Meeting, May 2008. (Abstract #4437, Poster

Discussion Session B24, Publication Page A307).

Kunkel,M., Gelfand,C., Liberchuk,R. "SNPStream MT: a medium throughput

genotyping system adapted for the Luminex 100".

-Presented at the American Society for Human Genomics conference,

Phila.

PA, 2000

Tocce, K., Suppiah, K., Rago, C., Liberchuk, R., Duffy, K., Riggs, J.

"DBA/2J (Mls-1a) B-cell Differentiation in BALB.xid Recipients".

Immunology 99:2000

Riggs, J., Duffy, K., Prior, T., Liberchuk, R."CD5+ & CD11b+ B Cell

Lymphoma Development with Aging"

-Presented at the NJ Commission on Cancer Research Annual Retreat,

NJ, 1999

Riggs,J.,Liberchuk,R. "IgM Production by DBA/2J B Cells after their

Transfer into BALB.xid mice".

-Presented at the American Association of Immunologists' conference,

Washington DC. 1999

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