LITTA STEPHEN

*** * ********* ****, *** #*** Phone: 650-***-****

Foster City, CA 94404-2109 acclua@r.postjobfree.com

OBJECTIVE

Seeking a challenging career in Molecular Biology with right blend of technical and management skills

EDUCATION

MTech in Biotechnology, Anna University of Technology, Chennai, 8.4 GPA May 2012

BTech in Biotechnology, Karunya University, Coimbatore, 8.2 GPA May 2010

TECHNICAL SKILLS

Technology: Mammalian Cell Culture, rDNA technology, Molecular Biology, Microbiology, Bioinformatics

Techniques: Plasmid construction, Gene cloning, Cell transfection, microbial fermentation, Protein purification,

HPLC,Plasmid Isolation, Gel Electrophoresis,SDS PAGE, siRNA,ELISA, PCR,RT-PCR,RNA DNA Isolation and purification

EXPERIENCE

June 2011-April 2012

Anna University, Chennai

Project Assistant

• Used Rat cell lines to study the mechanism involved in the progression of diabetes.

• Used RNAi mechanism, to suppress or knock out one or more genes involved in β cell death and hence the cells

escaped from senescence.

June 2010-July 2010

Mikrobiologisches Pruflabor GmbH (MPL), Innsbruck,Austria

Research Intern

• Tested medicines and vaccines from the pharma industries for sterility and if microbe is present, identified its type

by culturing it in different formulated media.

• Worked to the rules of Good Manufacturing Practice concerning to the WHO.

November 2009-April 2010

M S Swaminathan Research Foundation,Chennai

Research Assistant

• Analysed the mechanism for fatty acid synthesis in Jatropha curcus seeds and constructed a cDNA library.

• Cloned, amplified and sequenced genes, coded for protein synthesis and described a pathway for increasing the

yield of fatty acid.

• Hypothesized the over expression of the gene responsible for a specific protein synthesis will increase the secretion

of the oil in Jatropha seeds.

TRAINING

May 2009-June 2009

Ecolabs and Biotechnology Services, Chennai

• Learnt the techniques that are used for the easy degradation of large organic matters by vermicompost method.

April 2008-June 2008

Vivek Institute of Laboratory Medicine, Nagercoil

• Underwent the training programme and learnt the various biochemical as well as genetic analysis, pathological test,

harmone assays, Western Blot,cytogenetics, ELISA etc

Berkeley Extension,California

• Pursuing Certification Program in Advanced Bioscience.

Conference Attended

• Organized a National Level Technical Symposium”Evogen ‘09” in Karunya University,Coimbatore.

• Presented a poster”Waste water Treatment using Methanobacter” in a workshop on Biochemical Engineering

organized by the Department of Biotechnology,Karunya University.

• Presented a poster”Production of Biofuels using Microorganisms from low cost substrate” in INNOVAUTT

organized by Anna University Of Technology,Trichrappalli.

• Attended National Level Conference in New Horizons in Biotechnology,Bioinformatics And Food Technology.

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