Colette ap Rhys

Research Associate, Department of Neurosurgery

Baltimore, MD

acck0b@r.postjobfree.com - 410-***-****

Cell and Molecular Biologist with experience in cell culture, virology, protein purification and analysis, as well

as, bacterial and mammalian gene transfer technology. Recognized as an expert in the design and construction

of plasmids, recombinant viruses and viral vectors for research applications. Proficient in a wide range of

molecular biology techniques and analysis.

WORK EXPERIENCE

Research Associate, Department of Neurosurgery

Johns Hopkins University School of Medicine - Baltimore, MD - October 2011 to October 2012

Major area of research: the generation of retroviral and lentiviral vectors for in vivo mouse injections, as well

as, the transduction of brain tumor stem cells, mesenchymal stem cells and immortalized cell lines. These

vectors included expression cassettes encoding firefly and renilla luciferase for bioimaging studies, fluorescent

proteins for tagging cells, plus cDNAs and shRNAs for functional studies.

Instructor, Department of Neurosurgery

Laboratories of Dr. Gregory Riggins, M.D - April 2009 to October 2011

Major area of research: the design, construction, packaging and concentration of retroviral and lentiviral vectors

encoding cDNAs, shRNAs and miRNAs for members of the Department of Neurosurgery and Oncology.

Responsibilities include the teaching and training of postdocs, and graduate students.

Laboratories of Dr. Gregory Riggins, M.D., Ph.D., Dr. Alexandro Olivi, Dr. Alfredo Quiñones-Hinojosa, M.D.,

and Dr. Gary S. Hayward, Ph.D.

Manager of the Viral Vector Center, Instructor

Department of Neurosurgery - April 2007 to April 2009

Major area of research: producing vectors for the delivery of cDNA, shRNA and miRNA sequences to different

cell types in vitro and in vivo on a fee-for-service basis to the Hopkins community, other academic institutions

and Phizer. Responsibilities included training and supervision of lab personnel, generation of recombinant

lentiviral, retroviral, adenoviral and AAV vectors, and consultation in viral vector design and use. Additionally,

the core was under Phizer contract to supply a variety of shRNA knock down cell lines for cellular pathway

target validation.

Laboratory of Dr. David Johns, Ph.D.

HHMI Research Specialist

McKusick - Nathans Institute of Genetic Medicine - July 2000 to April 2007

Major areas of research: elucidating the mechanism of mammalian nonsense suppression (NMD), developing

viral tools to study NMD and examining Storm Syndrome at the cellular and biochemical level. Responsibilities

included advising graduate students in experimental design and troubleshooting failed experiments.

Manager of the Vector Core, Research Associate, Department of Oncology

Laboratory of Dr. Harry C. Dietz - July 1998 to July 2000

Major area of research: design and generation of recombinant herpes viruses, adenoviral, AAV and retroviral

vectors for clinical and basic scientists. Responsibilities included the production, purification and titering of

these viruses. Additional duties were the supervision of undergraduate students and a technician.

Manager of the Viral Core, Postdoctoral Fellow, Department of Pathology

Laboratories of Dr. William B. Guggino - November 1997 to July 1998

11/97 - 7/98

Major area of research: the design of adenoviral and AAV vectors for Human Gene Therapy, including

tetracycline and ecdysone regulated adenoviral vectors. Also, improvements in AAV production and purification

protocols were developed. Responsibilities included the supervision and training of a graduate student, a

technician and an undergraduate lab assistant.

Laboratory of Dr. Barry J. Byrne, M.D., Ph.D.

Intramural Research Training Associate, Laboratory of Molecular Genetics

National Institute on Aging, NIH - Baltimore, MD - October 1991 to October 1997

Major area of research: the elucidation of DNA damage induced stress pathways and the mechanisms of DNA

repair. Responsibilities included attending and presenting at scientific conferences, lecturing, writing scientific

proposals, book chapters and papers, as well as teaching, training and supervising a graduate student, a

postdoctoral student and high school summer students.

Laboratories of Dr. Nikki Holbrook, Ph.D. and Dr. Wilhelm Bohr, M.D., Ph.D.

EDUCATION

Ph.D. in Pharmacology and Molecular Sciences

Johns Hopkins School of Medicine Baltimore - Baltimore, MD

M.S. in Biological Sciences

University of Maryland Baltimore County Baltimore - Baltimore, MD

B.Sc. in Agriculture/Botany

University College of North Wales - Wrecsam

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