Engineer Development
Resume:
James Hyram Hamlin, Jr.
**** ******** ***., ***. **, St. Louis MO 63116
Phone: 770-***-****, Email: accjng@r.postjobfree.com
Professional Profile
Experience in immunology, tissue bioengineering, drug discovery, and biochemistry in both academic and
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industry settings
Significant experience in assay design, development, optimization and troubleshooting
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Enjoys group oriented work and communicating technical information
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Extensive experience in tissue based, cell based, and enzyme based assay execution
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Systematic approach to bench work and a well organized and detailed laboratory notebook
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Capable of maintaining a clean, well stocked, and efficient work space, as well as instructing and guiding
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junior associates
Education
Bachelor of Science in Biomedical Engineering Master of Science in Molecular & Genetic Medicine
Georgia Institute of Technology, Atlanta GA University of Sheffield, Sheffield England
GPA (08/99 07/04): 3.10 GPA (09/05 09/06): 76 (B+ on English grading scale)
Professional Experience
Research Associate III, Tissue Technology, Cryolife Kennesaw GA, May 2010 Dec 2013
Built on previous experience in a number of immunological assays, including immunohistochemistry,
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Western blot, and dot blot to characterize a variety of human and animal tissue antigens of interest
Devised ELISA, fluorescent, and extraction methods for qualification and quantification of zenograft
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glycoprotein and DNA fragment antigens to support new product development
Initiated intradepartmental human heart valve bioengineering effort in conjunction with Wake Forest
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Institute for Regenerative Medicine involving hybridoma antibody production and isolation
Evaluated vascular allograft biomechanical strength, denaturation characteristics, and histology, after
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exposure to novel cryoprotectants and controlled rate freeze protocols
Provided detailed tissue structure characterization from the microscopic to the gross scale in order to
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support engineer’s efforts at cardiac device design and modification
Executed a variety of tissue decellularization protocols followed by tissue biomechanical strength
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evaluations, thermal denaturation analysis, light microscopy qualitative assessment, and two photon
confocal microscopy determination of collagen and elastin integrity
Research Specialist, Hem/Onc. Dept., Emory University Atlanta GA, Sept. 2009 May 2010
Carried out a variety of molecular biology and cell biology assays to investigate role of protein of
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interest in development of acute myeloid leukemia
Designed siRNA to knockdown protein of interest in various cell lines to analyze resulting transcription
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factor expression
Executed and analyzed western blots and mRNA via rtPCR to determine knockdown efficiency
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Created truncations of protein of interest to determine binding affinity with associated proteins via
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immunoprecipitation and colocalization
Harvested mice bone marrow to determine degree of hematopoietic stem cell expansion as a result of
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pharmacological treatment
Research Associate II, Celtaxsys Inc, Atlanta GA, Sept. 2008 July 2009
Collaborated with drug discovery research team to pass priority compounds from activity screens, to
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efficacy and safety tests, and finally into animal models
Screened novel compounds and samples for migratory activity in various immune cells
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Executed secondary and tertiary verification assays on compounds which passed initial primary screens
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Assisted in the development and optimization of animal inflammatory models in mice
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Separated immune cell populations (neutrophils, T cells, monocytes, etc.) from whole blood to be used
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in cell based assays
Reviewed literature for novel sources of chemotaxic proteins for future migratory tests
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Improved existing standard operating procedures to increase efficiency and accuracy of assays
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Research Specialist, Pharm. Dept., Emory University, Atlanta GA, April 2007 Aug 2008
Managed large cancer research laboratory
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Developed several cell based, medium throughput assays for expression, binding, and localization of
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proteins of interest
Performed immunohistochemistry on over five hundred patient derived head/neck and lung cancer samples,
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while improving protocols to increase speed and quality of immunochemistry
Collaborated with immunologists, oncologists, pathologists, and statisticians to interpret, score, and analyze
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immunohistochemical stains
Created databases to track compounds, antibodies, and lab ware
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Graduate Researcher, Institute for Cancer Studies, University of Sheffield, Yorkshire England, 2006
Performed in vitro DNA replication, protein DNA binding, and protein protein binding assays with wild
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type, truncated, and point mutated proteins to make structure function relationships
Cultured large quantities of cells to harvest cytoplasm extracts and purified protein for future in vitro
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DNA replication assays
Authored thesis and presented research results on bovine papillomavirus DNA replication
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Undergraduate Researcher, Dept. of Biomed. Engr., Georgia Tech, Atlanta GA, 2002 2003
Developed sterile technique and basic laboratory protocols
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Became fluent in a variety of chemical assays modified to determine the efficiency of gene transfer
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(adeno virus to fibroblasts) as a model of gene therapy
Reported my results in a monthly lab meeting and authored a technical report summarizing my research
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Relevant Laboratory Techniques
Western blots Transfections Toxicity assay Tissue/Ab
o o
Immunopreci (transient, stable) Gel shift conjug.
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Sub cloning In vitro DNA
pitation assay o
Immunostaini Hybridoma PCR/rtPCR replication assays
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Microscopy
ng (IHC, ICC) Ab prod. Sequencing o
ELISA Protein (fluorescent, two
gels
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photon)
Immune Cell expression & RNA
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Lab animal
purification
isolation (PBMC, extraction o
Gel handling
PMN) Flow
Radioactive
Cell culture electrophoresis cytometry o
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Migration material handling
Bacterial Tissue
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assay
culture Dissections
References will be furbished upon request
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