James E. Agan

**** ******* **** **.

Las Vegas, Nevada 89135

accfgj@r.postjobfree.com

Home 702-***-****

Cell 702-***-****

Education

**** ******** ******** ********** *.A.

Major Microbiology

Minor Chemistry

Select Employment

4/12- current

Areva Solar Inc. Las Vegas, NV.

Process Engineer/Chemical Engineer- Consultant

• Electrochemistry of plating Ni and Sn on boiler tubes

• Chemistry of Solgel Protective coating on boiler tubes/electrical components

• Monitoring process of continuous production line in Manufacturing environment

• Using Real time statistical software Tableau to monitor process specifications and

indicators of potential problems

2/11-4/12 Part time

Nutri Pharmaceuticals Research Inc. Las Vegas NV

• Extraction of oils from powder using organic chemistry techniques

• Formulation of Products ranging from Emulsifiers for Petrochemical extraction to

Infant formula

• Making Specification sheets and Certificates of Analysis for customers

• Filling out customer regulatory and raw material questionnaires

• Conducting Microbiology Panels for COA

5/08- 5/09

Agan Food Mart Inc. Houston, TX.

Owner/ Operator

• Set up and maintained all business operations.

• Managed all the logistics of the business.

• Handled all the sales, inventory, and licenses.

3/06-5/08

Lexicon Pharmaceutical, Inc., Principal Research Associate – Biotherapeutics group

in Drug Discovery Team, The Woodlands, Tx.

• Stable transfection of cell lines for clonal selection

• Protein purification of secreted proteins by Affinity Chromatography quantification,

preparation of antigen for immunization into KO Mice

• Immunization and titer of mice by ELISA and 2D multiscreen western

• Epitope Mapping and binning of hybridoma subclones by Competition ELISA.

• Kinetic and funtional assay’s

• Analysis of Data using Excel, Prism software for graphs and Powerpoint

presentation

2/05-2/06

Lexicon Genetics, Inc., Principal Research Associate – Genomics

Manager of project-specific genotyping; Liaison to Molecular Genetics

• Oversee the organization of over 100 GS-PCR data files for NIH

• Optimize Lexicon’s Genotyping logistics reducing the turn around

time from 4 to 2 days

• Oversee Quality Assurance for mouse production

• Identify, investigate, and report QA failures to maximize the

productivity of Genome5000 project

• Supervise 3 Research Associates performing complex genetic analysis

• Oversee all the gene-specific strategy implementation and the

organization of genotyping materials received from the Molecular Genetics

group

7/01-2/05

Lexicon Genetics, Inc., Senior Research Associate – Genomics

(8/04) Manager of project-specific genotyping; Liaison to Molecular Genetics

• Develop and implement high throughput methodologies for QA

testing of Lexicon’s mice

• Perform high-throughput PCR and QPCR assays for genotyping

and quality assurance of client projects

• Troubleshoot technically challenging projects; identify, investigate

and report QA failures

1996-2001

Baylor College of Medicine, Research Technician 2-3

Department of Molecular and Cellular Biology

• Oversee neonatal rat, mouse, and 14 day chicken embryo primary

cardiomyocyte and myoblast tissue culture

• Create, maintain and characterize stable mammalian cell lines to

support TNFα and muscle wasting experimentation

• Researched several transcription factors that modulate alpha actin

expression in differentiating myoblasts

1994-1996

Park Place East, Assistant Property manager

• Showed property to potential tenants

• Had tenants sign lease and put down deposit

• Took care of problems that came up, maintenance, and prepared rooms for new Tenants

1992-1996

Southern Illinois University at Carbondale, Undergraduate Assistant

Department of Biochemistry

• Isolated Sarcoplasmic Reticullum from deep sea scallop, extracted, quantitated

concentrations by Bradford assays and purified proteins by HPLC. Ran

polyacrylamide gels.

• Also assisted in screening cDNA libraries.

1989-1990 Veterinarian Technician

Belleville Animal Clinic

• Dosed animals orally and sub Q as needed with prescribed

medications

• Performed blood draws from dogs

Military Experience / Management Experience

1996-1999 Sergeant (E-5)

Mississippi National Guard 20th Special Forces Group 2nd Battalion C Co.

• Taught classes in basic soldier skills focusing mainly on planning

and conducting small unit operations

1984-1988 Sergeant (E-5)

1st Ranger Battalion

U.S Army

• Trained to conduct direct action and lead special operation missions

• Ranger qualified, Jumpmaster qualified, Airborne qualified

• Commanded teams of 3-8 soldiers

Computer Skills

Microsoft Office (Word, Excel, PowerPoint), Tableau, Adobe Photoshop, DNAStar

suite, Oligo6, Sequencher, Primer express, ImageQuant, Blast algorithms, online

informatics resources (Ensembl, NCBI, OMIM, Unigene, SMART, etc)

Publications

Lei Wei, Lu Wang, James A. Carson, James E. Agan, Kyoko Imanaka-Yoshida and Robert

J. Schwartz (2001) B1 Integrin and Organized Actin Filaments Facilitate Cardiomyocyte-

specific RhoA-dependent Activation of the Skeletal -actin Promoter. The FASEB journal

Vol.15

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