A KI H OJI, P h.D

!

Rm**** Research Pavilion Parran Hall, GSPH-IDM, 130 Desoto, Pittsburgh, Pennsylvania 15261

!

310-***-**** acccz6@r.postjobfree.com

P ROFESSIONAL S UMMARY

Possess more than 8 years of comprehensive biomedical research experiences in NIH funded academic

research laboratories. Have successfully developed and completed technically challenging projects,

participated in several federally funded projects, and have written portions of several NIH/NIAID grant

applications based on my graduate studies and submitted NIH grant applications during postdoctoral studies.

A highly trained, self-motivated, and efficiency-driven scientist comfortable in multifaceted environments.

!

OVERALL QUALIFICATIONS

!

Highly skilled research scientist and published author adept in multi-color flow cytometry,

•

immunoassays, protein chemistry and molecular biology techniques.

Expert Immunologist, especially in the area of cellular immunology and immunotherapy development.

•

Expert in design, development, and evaluation of cell-based and immunological assays.

•

Expert in computer skills relevant to flow cytometric and statistical analysis as well as subsequent data

•

visualization.

Expert in software and hardware integration of various modern flow cytometers.

•

Proficient in relevant literature searches and review current/updated information.

•

Supervised and trained several graduate students, medical fellows and research assistants.

•

Established trouble shooting and problem solving skills.

•

Worked in the flow cytometry core facility under FDA and NIH guidelines and regulations and

•

supervised several graduate students and core facility technicians.

Member of International Society of Analytical Cytometry.

•

! !

PROFESSIONAL EXPERIENCE

UNIVERSITY OF PITTSBURGH, Pittsburgh, Pennsylvania 2010 - Current

Research Scientist

• Establishing a B cell transcription factor network by 16 parameter flow cytometric analyses

• Manipulation of Epstein Bar Virus Bacterial Artificial Chromosome

• Characterizing human B lymphocyte phenotypic subsets by 13 parameter flow cytometirc analyses

• Characterizing of Epstein Barr Virus latency protein functions in human B lymphocytes

• Developing novel intracellular nano-probes for polychromatic live cell sorting

• Developing integrated analysis of functional genomics and flow cytometric data

!

UNIVERSITY OF PITTSBURGH, Pittsburgh, Pennsylvania 2008 - 2010

Research Associate

• Established modern 8-color immune monitoring system for glioblastoma vaccine trial.

• cytometry for the cancer immunotherapy/vaccine project.

• Developing ex vivo viral and non-viral gene delivery and persistent gene expression strategies for

human T lymphocytes.

• Investigating gene knockdown strategies to increase functionalities of human T lymphocytes.

!

CEDARS SINAI MEDICAL CENTER, Los Angeles, California 2008

Technical Consultant

• Strengthened an immunological monitoring program for the glioma immunotherapy/vaccine project.

• Established SOP using modern immunological assays based on polychromatic flow cytometry.

• Trained and supervised research scientists for assay development and trouble shooting.

• Participated in NIH R01 and SBIR grant development.

• Improved overall laboratory operations and environment.

!

Page 2

!

! MEDICAL CENTER, Los Angeles, California

UCLA 2005 – 2008

Postdoctoral Fellow

• Supervised graduate students and medical fellows and provide technical assistance on flow cytometry to

lab personals.

• Optimized a method for in vitro antigen-specific CD8+ and CD4+ T cell priming by monocyte-derived

dendritic cells and Langerhans cells.

• Managed two main projects characterizing regulatory T cells using 9-color flow cytometry and FACS

sorting, which resulted in one poster presentation in the international conference and three manuscripts

and another is in preparation for publication.

!

UNIVERSITY OF PITTSBURGH, Pittsburgh, Pennsylvania 1995 – 2005

Graduate Student

Participated in an NIH funded dendritic cell based HIV-1 vaccine project.

•

Supervised junior and senior level graduate students, consulted several investigators on their NIH funded

•

projects, and helped run a departmental flow cytometry core facility.

Utilized 6 color flow cytometric analysis to study phenotypic assessment of HIV-1 specific CD8+ T cell

•

memory-effector differentiation by using MHC class I tetramers and IFN-γ ICS and ELISPOT assay.

Determined that functional capacities of the central memory EBV-specific CD8+ T cells produced IFN-γ

•

and TNF-α in a six-hour peptide-specific stimulation.

Examined influenza A-specific CD8+ T cells phenotypically and functionally using ex vivo staining by

•

tetramers specific for epitopes and by IFN-γ ICS.

Developed a method for efficient and inexpensive in-house production of MHC class I tetrameric

•

staining reagents.

E D U C AT I O N

!

UNIVERSITY OF PITTSBURGH, Pittsburgh, Pennsylvania

Research Associate, 2010

!

UNIVERSITY OF CALIFORNIA-LOS ANGELES, Los Angeles, California

Postdoctoral Fellow, 2007

!

UNIVERSITY OF PITTSBURGH, Pittsburgh, Pennsylvania

Doctor of Philosophy in Viral Immunology, 2005

!

UNIVERSITY OF WISCONSIN-MADISON, Madison, Wisconsin

Bachelor of Science in Molecular Biology and Zoology, 1995

!

TECHNICAL SKILLS

!

Flow Cytometry Related

Software:

! System II, Expo32, CXP, Cytomation Summit, BD FACS DIVa, Winlist, and FlowJo

Coulter

!

Hardware:

Coulter Epics Elite, XL, FC500, Cytomation MoFlo, BDFACS Canto, LSRII (HTS), Fortessa and Aria II+

!

Methods:

Surface, Intracellular and Intranuclear Staining, Cytokine ICS, MHC Class I and II Tetramer Staining,

Fluorescent Dye (e.g. CFSE) labeling, Tyramid Signal Amplification, HTS cytokine detection, Apoptosis

detection, Fluorescent dye based proliferation assay, Polychromatic (up to 18-color) flow cytometry, Flow

Page 3

!

CTL assay, Cell Cycle analysis, Phospho-Flow, Biohazard Cell Sorting (BSL2+), and and rare event

detection and analysis

! Technical Skills

Other

Tissue Culture:

BSL2+ and 3 experiences, Preparation of human peripheral blood mononuclear cells by Ficoll-Histopaque,

and BD CPT tubes, rimary human cells (including dendritic cells, lymphocytes and monocytes) and various

human and mouse cell lines and clones, cloning of primary human CD8 T lymphocytes.

!

Immunoassays:

ELIZA, ELISPOT, Luminex and Flow Cytometry bead cytokine multiplex assays, Magnetic beads (MACS

and Dynal) separation.

!

Molecular Biology:

Various PCR bistros, qPCR (Taqman system), SA Biosciences PCR Arrays, cloning and construction of

various non-viral (e.g. sleeping beauty vectors) and viral (lentivirus) expression vector and Bacterial

Artificial Chromosomes (by lambda RED recombination system), transfection by Amaxa Nucleofacor,

lentiviral packaging and transduction, Next Generation Sequencing based gene expression data analyses

(CLC Genomic Work Bench 5).

!

Miscellaneous Technical Skills:

SDS-PAGE, Western blot (also by iBlot), Prokaryotic Protein Expression and Purification, FPLC,

Fluorescent Microscopy, Human T cell transfection, Lentiviral production and transduction

! Computer and Statistical Skills:

Relevant

Apple Macintosh (OSX), MS Windows (Win 7 Ultimate, NT, XP and Vista), and Linux

JMP, Aable, PRISM, and R package for statistical analyses and data visualization of high diminutional data

!

PROFESSIONAL SKILLS

Assay Development

• Ex vivo antigen-specific stimulation primary human T cells and subsequent detection of multiple

cytokines by multiparameter flow cytometry and a Luminex platform.

• In vitro stimulation of primary human monocyte derived dendritic cells and plasmacytoid dendritic cells

by multiple Toll Receptor Ligands and subsequent detection of multiple cytokines by multiparameter

flow cytometry and a Luminex platform.

• Effects of multiple miRNAs on IL-2 production by Jurkat cells stimulated by PMA and Ionomycin in

vitro.

• Effects of cell penetrating peptide reagents on 293T, and Jurkat cell lines and human primary T and

monocyte derived dendritic cells in vitro.

!

Management Skills

• Have mentored undergraduate, graduate students, and medical fellows.

• Lead and trained a team of two research assistances for U01 grant at UCLA.

• Lead and trained two research associate for the immune-monitoring program at Cedars Sinai Medical

Center.

• Lead and supervised a flow cytometry based immunomonitoring program for brain cancer vaccine

clinical trials.

!

Bioinfomatics Skills

• Read mapping and subsequent analysis of next generation sequencing results of EBV genome by using

CLC Genomic Workbench

• Read mapping and subsequent analysis of human exosomal trascriptomes by using CLC Genomic

Workbench and Tophat2+Bowti2+DESeq2/Limma-Voom.

• Data visualization of high volume and dimensional flow cytometric data by using R and SAS/JMP.

Page 4

!

Flow Cytometry Technical Consultation

• 18-color phenotypic panel development and data analysis of human bronchial lavage leukocytes (2006-

current, ongoing)

• 6-color phenotypic panel development and data analysis of rhesus macaques PBMCs and LN cells

(2012)

• 12-color phenotypic panel development of human colonic dendritic cell phenotype (2012)

• 8-color phenotypic and functional panel development and data analysis SOP for human glioblastoma

clinical trial (2008)

!

P RECOGNITION

ROFSSIONAL

!

NIAID Fellows and New Investigator Scholarship, HIV Pathogenesis, Keystone, Colorado, 2006

! Fellow Travel Grant for the Keystone Symposium, Molecular Aspects of Viral Immunity, Keystone,

NIAID

Colorado, 2001

!

Co-Principle Investigator, NIH/NIAID U01 AI35040, “Novel Immune Control of Persistent HIV-1

Viremia by HLA-B*5701 allele”, 2005-2007

!

!

!

!

!

!

Contact this candidate