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Cellular and Molecular Biology Researcher

Location:
San Francisco, CA
Posted:
June 20, 2014

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Resume:

OBJECTIVE:

I am eager to be part of a company using evidence-based science and innovation to develop early diagnosis and/or effective treatment of human diseases.

CANDIDACY SNAPSHOT

PROFESSIONAL ACCOMPLISHMENTS:

Conceived and conducted to completion a novel project bringing new conceptual views and expanding the scientific scope of the research group Developed and optimized human stem cell differentiation protocols Generate a mouse strain and successfully troubleshot technical issues on many others Assisted in the implementation of genetically modified mouse strain production

Set up the management and maintained a transgenic mouse colony effectively reducing costs and unnecessary production of animals

Designed prototypes for tissue processing devices Assisted on patent applications: drafting patents, designing figures and researching prior art.

ATTRIBUTES:

RESEARCH COMPETENCE AND APTITUDE:

Design and conduct research from grant writing to publication Strong collaboration skills Judicious data analysis Tenacious problem solver Innovation advocate

TECHNICAL SKILLS: Assay development Human and mouse stem cell culture (ES-cell, iPS-cell, mesenchymal and hematopoietic stem cells) Mouse xenograft bone marrow reconstitution and teratoma formation assays Colony forming cell assay Mammalian cell culture: maintenance, transfection, transduction, microinjection and primary cell line production Flow cytometry - FACS, surface and intracellular phenotyping Genetically-modified mouse strain production and characterization Small rodent surgery Mouse monoclonal antibody production Immunohistochemistry ELISpot assay Radioimmunoassay RTqPCR Southern, Western and Northern blotting Gene cloning, plasmid design and construction Laboratory information management systems (LIMS) use Current good manufacturing practice (cGMP) knowledge Clinical trial process familiarity Device design Patent writing and application experience

COMPUTER LITERACY: Regularly use office suites and analysis software in Mac OS, Windows and Ubuntu (Linux) systems, as well as web based analysis tools (such as the ones in NCBI, UCSC, EMBL, Galaxy, etc).

TEACHING AND MENTORING

I have trained research assistants, students and postdoctoral fellows in specific techniques; co-supervised master and undergraduate students in their thesis projects; and taught middle school science classes.

LANGUAGES: Fluent in English and Portuguese, and able to communicate well in Spanish.

PROFESSIONAL INFORMATION

EDUCATION

SC.D. BIOLOGICAL SCIENCES - MOLECULAR BIOLOGY - UNIFESP - Brazil - 2008

B.S. BIOLOGICAL SCIENCES - Universidade de São Paulo - Brazil - 1997

PROFESSIONAL EXPERIENCE

2014 - PRESENT SCIENTIST • SAGEMEDIC, INC.

Providing expertise on tissue processing methods, stem cell and cancer biology, and cell biology technologies for the development of in vitro cell-based assays to guide personalized cancer therapy; designing device prototypes; drafting patents and researching prior art.

2012 - 2014 POSTDOCTORAL FELLOW • BLOOD SYSTEMS RESEARCH INSTITUTE /UNIVERSITY OF CALIFORNIA SAN FRANCISCO

Postdoctoral fellowship: UCSF Hematology and Oncology NRSA training grant (T32)

Developed methods to differentiate human induced pluripotent stem cells into hematopoietic stem cells, explored the use of mesenchymal cells to maintain and expand ex-vivo human fetal hematopoietic stem cells and collaborated intensively on many experiments as part of a multi-lab NIDDK funded program project that aims at developing a combined stem cell and genetic based therapy for hemoglobinopathies.

2010 - 2011 MOLECULAR BIOLOGY RESEARCH CONSULTANT • SÃO PAULO • BRAZIL

Gave guidance, supervision and advice on graduate students projects that aimed at: gaining better understanding on the molecular and cellular mechanisms that underlie the derailed cell differentiation response seen in the Fibrodysplasia Ossificans Progressiva disease and its relation to inflammatory signaling pathways; improve the efficiency and cost of patient genetic diagnosis of Fabri Disease; evaluate in a rodent model of metabolic syndrome disease the potential use of adipose tissue derived stromal cells as therapeutic agents. Also rendered consultation on genetically-modified mouse strain maintenance.

2009 - 2010 POSTDOCTORAL FELLOW • MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE

Prof. Michael Bader, Molecular Biology of Peptide Hormones, Berlin, Germany

Postdoctoral fellowship: CAPES-PROBRAL, Ministério da Educação do Brasil

Besides conducting gene expression analysis on samples from kinin receptors B1 and B2 knockout mice to further elucidate the roles kinin B1 and B2 receptors play in endothelial cell biology, I used my expertise in genetically modified mouse strain production and characterization to generate a conditional knockout mouse strain for Elastase 2a gene (Ela2a), and developed the genomic DNA based genotyping protocols for Thimet oligopeptidase and Neurolysin gene trap knockout mouse strains, as well as began the phenotype analysis of theses strains.

2003 - 2008 DOCTORAL FELLOW • UNIVERSIDADE FEDERAL DE SÃO PAULO • BRAZIL

Mentor: Prof. Dr. João Bosco Pesquero, Biophysics Department

Thesis: Transcription from the mouse Carboxypeptidase M locus

Doctoral Fellowship: CAPES-PROEX, Ministério da Educação do Brasil

During my doctorate I conceived and conducted an entire new research project in the group, born out of the necessity to define the genomic structure of the mouse Carboxypeptidase M (CPM) locus in order to design gene targeting plasmids. I contributed to expand the scientific scope and conceptual estate of the lab by analyzing the transcription from the mouse Carboxypeptidase M locus, both protein coding and non-coding RNAs, as well as the impact on the neighboring gene transcription, MDM2. The knowledge on expression analysis and transcription context, along with my stem cells and mouse genetics experience, enabled me to contribute significantly to other projects within the lab and with other groups in Brazil, such as: studies on human and rodent neuronal cell differentiation, expression analysis of kinin-kallikrein system components in disease states and implementation of the knockout mouse production

2000 - 2003 RESEARCH ASSISTANT II • HARVARD UNIVERSITY

Laboratory of Prof. Frank McKeon, Harvard Medical School, Boston, USA

My first contribution to the laboratory was to set up the management and maintenance of transgenic mice colony. While generally assisting lab members in nany experiments, I got deeply involved with the projects on the p53 family members p63 and p73. I gained extensive experience on DNA sequence analysis while cloning and initially characterizing both p63 and p73 proximal and distal promoters. I also extended considerably my technical skills in molecular biology and cell culture while performing many tasks, such as: designing and constructing gene targeting plasmids to generate conditional knockout mouse strains, isolating and culturing human foreskin and mice skin keratinocytes and fibroblast, mammalian cell line transfection and transduction, Luciferase assays, producing and screening new mouse monoclonal antibodies, and generating new mouse embryonic stem cell lines.

1997 - 2000 TECHNICAL RESEARCH ASSISTANT • BRIGHAM AND WOMEN’S HOSPITAL

Lab. of Prof. Richard M. Mortensen, Endocrine-Hypertension Div., Boston, USA

In professor Mortensen's lab, besides assisting in the continuance of the laboratory’s embryonic stem cell lines and their differentiation into cardiomyocyte-like cells, I mastered how to manage a transgenic mouse colony and performed many experiments and data analysis to phenotypically characterize the knockout mouse strains, such as: surgically implant ECG sensor in mice, acquire and analyze the data obtained; perform mice glucose and insulin tolerance tests; serum insulin RIA measurements; production of neonatal mice cardiomyocytes and hyppocampal cell primary cultures.

2010 - 2011 MOLECULAR BIOLOGY RESEARCH CONSULTANT • SÃO PAULO • BRAZIL

Gave guidance, supervision and advice on graduate students projects that aimed at: gaining better understanding on the molecular and cellular mechanisms that underlie the derailed cell differentiation response seen in the Fibrodysplasia Ossificans Progressiva disease and its relation to inflammatory signaling pathways; improve the efficiency and cost of patient genetic diagnosis of Fabri Disease; evaluate in a rodent model of metabolic syndrome disease the potential use of adipose tissue derived stromal cells as therapeutic agents. Also rendered consultation on genetically-modified mouse strain maintenance.

2009 - 2010 POSTDOCTORAL FELLOW • MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE

Prof. Michael Bader, Molecular Biology of Peptide Hormones, Berlin, Germany

Postdoctoral fellowship: CAPES-PROBRAL, Ministério da Educação do Brasil

Besides conducting gene expression analysis on samples from kinin receptors B1 and B2 knockout mice to further elucidate the roles kinin B1 and B2 receptors play in endothelial cell biology, I used my expertise in genetically modified mouse strain production and characterization to generate a conditional knockout mouse strain for Elastase 2a gene (Ela2a), and developed the genomic DNA based genotyping protocols for Thimet oligopeptidase and Neurolysin gene trap knockout mouse strains, as well as began the phenotype analysis of theses strains.

2003 - 2008 DOCTORAL FELLOW • UNIVERSIDADE FEDERAL DE SÃO PAULO • BRAZIL

Mentor: Prof. Dr. João Bosco Pesquero, Biophysics Department

Thesis: Transcription from the mouse Carboxypeptidase M locus

Doctoral Fellowship: CAPES-PROEX, Ministério da Educação do Brasil

During my doctorate I conceived and conducted an entire new research project in the group, born out of the necessity to define the genomic structure of the mouse Carboxypeptidase M (CPM) locus in order to design gene targeting plasmids. I contributed to expand the scientific scope and conceptual estate of the lab by analyzing the transcription from the mouse Carboxypeptidase M locus, both protein coding and non-coding RNAs, as well as the impact on the neighboring gene transcription, MDM2. The knowledge on expression analysis and transcription context, along with my stem cells and mouse genetics experience, enabled me to contribute significantly to other projects within the lab and with other groups in Brazil, such as: studies on human and rodent neuronal cell differentiation, expression analysis of kinin-kallikrein system components in disease states and implementation of the knockout mouse production

2000 - 2003 RESEARCH ASSISTANT II • HARVARD UNIVERSITY

Laboratory of Prof. Frank McKeon, Harvard Medical School, Boston, USA

My first contribution to the laboratory was to set up the management and maintenance of transgenic mice colony. While generally assisting lab members in nany experiments, I got deeply involved with the projects on the p53 family members p63 and p73. I gained extensive experience on DNA sequence analysis while cloning and initially characterizing both p63 and p73 proximal and distal promoters. I also extended considerably my technical skills in molecular biology and cell culture while performing many tasks, such as: designing and constructing gene targeting plasmids to generate conditional knockout mouse strains, isolating and culturing human foreskin and mice skin keratinocytes and fibroblast, mammalian cell line transfection and transduction, Luciferase assays, producing and screening new mouse monoclonal antibodies, and generating new mouse embryonic stem cell lines.

1997 - 2000 TECHNICAL RESEARCH ASSISTANT • BRIGHAM AND WOMEN’S HOSPITAL

Lab. of Prof. Richard M. Mortensen, Endocrine-Hypertension Div., Boston, USA

In professor Mortensen's lab, besides assisting in the continuance of the laboratory’s embryonic stem cell lines and their differentiation into cardiomyocyte-like cells, I mastered how to manage a transgenic mouse colony and performed many experiments and data analysis to phenotypically characterize the knockout mouse strains, such as: surgically implant ECG sensor in mice, acquire and analyze the data obtained; perform mice glucose and insulin tolerance tests; serum insulin RIA measurements; production of neonatal mice cardiomyocytes and hyppocampal cell primary cultures.

Alessander de Oliveira Guimaraes, Sc.D. accc63@r.postjobfree.com

Molecular and Cellular Biosciences Scientist Phone: 805-***-****

PATENT APPLICATION

Assays to Guide Personalized Therapy, US provisional patent application number: 62005620 (2014)

PUBLICATIONS

PEER REVIEWED PUBLICATIONS

Guimarães AO, Motta FL, Alves VS, Castilho BA, Pesquero JB. Multiple RNAs from the mouse Carboxypeptidase M locus: functional RNAs or transcription noise? BMC Mol Biol. 2009. 10:7.

Schwindt TT, Motta FL, Gabriela F B, Cristina G M, Guimarães AO, Calcagnotto ME, Pesquero JB, Mello LE. Effects of FGF-2 and EGF removal on the differentiation of mouse neural precursor cells. An Acad Bras Cienc. 2009 Sep . 81(3):443-52.

Schwindt TT, Motta FL, Barnabé GF, Massant CG, Guimarães Ade O, Calcagnotto ME, Conceição FS, Pesquero JB, Rehen S, Mello LE. Short-term withdrawal of mitogens prior to plating increases neuronal differentiation of human neural precursor cells. PLoS One. 2009 . 4(2):e4642.

Hara DB, Leite DF, Fernandes ES, Passos GF, Guimarães AO, Pesquero JB, Campos MM, Calixto JB. The relevance of kinin B1 receptor upregulation in a mouse model of colitis. Br J Pharmacol. 2008 Jul . 154(6):1276-86.

Martins AH, Alves JM, Trujillo CA, Schwindt TT, Barnabé GF, Motta FL, Guimaraes AO, Casarini DE, Mello LE, Pesquero JB, Ulrich H. Kinin-B2 receptor expression and activity during differentiation of embryonic rat neurospheres. Cytometry A.2008 Apr.73(4):361-8.

Ferro ES, Carreno FR, Goni C, Garrido PA, Guimaraes AO, Castro LM, Oliveira V, Araujo MC, Rioli V, Gomes MD, Fontenele-Neto JD, Hyslop S. The intracellular distribution and secretion of endopeptidases 24.15 (EC 3.4.24.15) and 24.16 (EC 3.4.24.16). Protein Pept Lett. 2004 Oct . 11(5):415-21.

MEETING ABSTRACTS

Suzuki S, R Geoffrey Sargent RG, Esmaeili-Shandiz A, Yezzi MJ, Illek B, Fisher H, Muench MO, Beyer AI, Guimaraes AO, Ye L, Chang J, Fine EJ, Cradick TJ, Bao G, Kan YW, Gruenert DC. Directed differentiation of CF-iPS cells into genetically and functionally corrected airway epithelia and inflammatory cells. Submitted to: 28th Annual North American Cystic Fibrosis conference, Atlanta, 2014.

Suzuki S, R Geoffrey Sargent RG, Esmaeili-Shandiz A, Yezzi MJ, Illek B, Fisher H, Muench MO, Beyer AI, Guimaraes AO, Fine EJ, Cradick TJ, Bao G, Gruenert DC. Footprint-free TALEN-mediated SDF correction of genomic CFTR and CFTR function in Cystic Fibrosis iPS Cells. American Society of Gene & Cell Therapy 17th Annual Meeting, Washington DC, 2014.

Casarotto PC, Becari C, Diniz CRAF, Guimaraes FS, Salgado MC, Guimarães AO, Salgado HC, Joca SRL, Bader M, Pesquero JB. Behavioral profile of Elastase 2A knockout mice: increased repetitive behavior. In: 11th International Bassal Ganglia Society Meeting, Israel, 2013.

Guimarães AO, Beyer AI, Wilkinson MB, Ye L, Suzuki S, Gruenert DC,Kan YW, Muench MO. CD133 is a good human ES and iPS cell marker of pluripotency and of hematopoietic differentiation capacity. SELECTBIO Inaugural Clinical Translation of Stem Cells Summit and Clinical Practicum, Palm Springs, 2013.

Beyer AI, Guimaraes AO, Ye L, Kan YW, Muench MO. Human ES & iPS cell CD133 & CD326 expression dynamics mimic commonly used pluripotency markers upon onset of differentiation despite differences among cell types. SELECTBIO Inaugural Clinical Translation of Stem Cells Summit and Clinical Practicum, Palm Springs, 2013.

Becari C, Durand MT, Martins Dias DP, Lataro RM, Oliveira M, Fazan Jr R, Guimarães AO, Pesquero JB, Bader M, Salgado MCO, Salgado HC. Knockout Mice for Elastase-2, a Novel Angiotensin II Generating Enzyme, Displayed Reduced Sympathetic Modulation of Arterial Pressure and Heart Rate. High Blood Pressure Research, Scientific Sections Abstracts, p. 212-213, 2013.

Wilkinson MB, Beyer AI, Guimaraes AO, Muench MO. Mantaining and expanding hematopoietic stem cells with the use of stromal cells. CIRM - Bridges Trainee Meeting, San Francisco, 2013.

Fernandes L, Loiola RA, Hilzendeger AM, Guimaraes AO, Reis F, Kawamoto E, Scavone C, Abdalla D, Bader M, Pesquero JB. Targeted Disruption of Kinin B1 Receptor Gene Alters Vascular Reactivity and Reduces Nitric Oxide Availability. SFRBM's 17th Annual Meeting, Orlando, 2010. Free Radical Biology and Medicine v.49. p.S21 – S21, 2010.

Turaça LT, Guimaraes AO, Araujo RC, Semedo P,Camara NO, Pesquero JB. Cell therapy in ob/ob mice using mesenchymal stem cells from mouse white adipose tissue. Brazilian Society for Cell Biology, 2010, São Paulo. XV Meeting of the Brazilian Society for Cell Biology. p.138-138, 2010.

Loiola RA, Guimaraes AO, Reis F, Hilzendeger AM, Kawamoto EM, Scavone C, Abdalla DSP, Bader M, Pesquero JB, Fernandes L. Nitric Oxide Alterations in Kinin B1 Receptor Knockout Mice. The 14th Annual Meeting of The European Council for Cardiovascular Research. v.54. p.1164 - 1187, 2009.

ADITIONAL INFORMATION: http://www.linkedin.com/pub/alessander-guimaraes/35/7a3/4ba



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