ROHAN KAUSHAL Email:

acc83q@r.postjobfree.com

*** ******* ***, *** #*

859-***-****

Lexington, KY 40508

SUMMARY:

Biomedical engineer with a 6+ years of global experience in research, data analysis and manufacturing of biopharmaceuticals. A unique and demonstrated background, spanning over multiple disciplines such as molecular & cell biology, data analysis and management, behavioral neuroscience, pain research (in vivo models), tissue culture & histology, protein analytics, neurosurgery, pharmacology and analytical toxicology.

EDUCATIONAL BACKGROUND:

. University of Kentucky

Master of Science (M.S.), Medical Sciences GPA

3.48/4.0

Aug, 2009-Dec, 2013

. University at Buffalo, The State University of New York

Master of Science (M.S.), Biotechnology GPA

3.45/4.0

Aug, 2007-Sept, 2009

. Kurukshetra University

Bachelor of Technology (B.Tech), Biotechnology

GPA 3.87/4.0 Aug, 2002-June, 2006 (First class, Honors)

RESEARCH PROJECTS:

. Worked on, "Studying the role of locus coeruleus (A6) noradrenergic

neurons in the facilitation of chronic orofacial neuropathic pain" at

University of Kentucky

. Worked on, "Reconstruction of the nigrostriatal dopamine pathway using

axon regeneration and embryonic tissue micro transplantation in an

adult parkinsonian rat" at University of Kentucky

. Worked on, "Optimization and Standardization for the low level

detection of polybrominated diphenyl ethers (PBDE's) using GC-ECD" at

Toxicology Research Center, SUNY At Buffalo

RESEARCH SKILLS & LABORATORY TECHNIQUES:

. Instrumentation: Calibration and conditioning of GC columns,

acquainted with extraction protocols and installation of GC columns,

Operating GC-ECD & GC-MS, pH Meter, spectrophotometry, NanoDrop, HPLC,

TLC, ION-Exchange chromatographic unit, Light microscope, Phase

contrast microscope, Fluorescent microscope

. Surgeries: Infra orbital chronic constriction injury (ION-CCI),

Stereotaxic surgeries for pathway reconstruction, Cranial cannula

implantation, Tissue transplantation, drug delivery using infusion

pumps through cannula, perfusion and organ harvesting.

. Rodent Behavior: Von frey fiber testing, open field testing, light

dark box, Hot & Cold plate testing, cylinder test

. Molecular Biology: Lentivirus and AAV production, PCR, Western

blotting and quantitative analysis using LICOR-Odyssey, SDS-PAGE,

Agarose gel electrophoresis and Gel analysis, RNA extraction, DNA and

plasmid isolation and quantitation, Gene cloning, Transformation,

Protein expression, isolation and purification

. Immunology: ELISA, Immunohistochemistry (IHC), Cell counting, Tissue

Cell culture

INDUSTRY WORK EXPERIENCE:

Organization Period

Position

Panacea Biotec. Ltd., India Aug, 06 - July, 07 Tr.

Scientific Officer

. Established a state of art vaccines manufacturing facility

. Prepared and revised user required specification, design

qualification, standard operating protocol and batch production

records

. Installed and commissioned various equipments for filling and

formulation of vaccines

Orchid Biomedical, Goa, India Summer 2005 Engr.

Intern

. Manufacturing of rapid diagnostic kits like HIV (ELISA Kits), Malaria,

Hepatitis etc

Alembic Pharmaceuticals, India Summer 2004 Engr.

Intern

. Strain improvement of S. erythraeus and antibiotic production

(Erythromycin) at pilot plant level

AWARDS AND HONORS:

2012 Travel award for Society for Neuroscience (SFN), 2012 by Graduate

School and Department of Physiology, UK, USA

2012 Kentucky Opportunity Fellowship for the 2012-2013 academic year

2009 Kentucky Opportunity Fellowship for the 2009-2010 academic year

PUBLICATIONS:

Master Thesis: Optimization and standardization of GC-muECD method for the

low level determination of seven polybrominated diphenyl ethers (PBDEs)

found in serum sample.

http://gradworks.umi.com/14/69/1469091.html

Kaushal, R., Zhang, L., Taylor, B.K., and Westlund, K. N. (In Preparation).

Selective Elimination of Locus Coeruleus Noradrenergic Neurons Alleviates

Chronic Orofacial Pain.

POSTER PRESENTATIONS:

*R.H. Kline IV, *R. Kaushal, A. Jamal, K.N. Westlund (2013) Chronic

constriction injury of the infraorbital nerve produces anxiety-like

behaviors in the rat

8th Annual CCTS Spring Conference University of Kentucky Center for

Clinical and Translational Science Monday, April 8, 2013

*equal contributing authors

Rohan Kaushal, Fei Ma, Liping Zhang, Bradley K. Taylor and Karin N.

Westlund (2012)

Knockdown of Locus Coeruleus Noradrenergic Neurons Alleviates Chronic

Orofacial Pain

22nd Annual Neuroscience Day, Louisville Chapter, April 19, 2012

Rohan Kaushal, Fei Ma, Liping Zhang, Bradley K. Taylor and Karin N.

Westlund (2012)

Knockdown of Locus Coeruleus Noradrenergic Neurons Alleviates Chronic

Orofacial Pain

18th Annual Kentucky Spinal Cord & Head Injury Research Trust (KSCHIRT)

Symposium May 11, 2012

Rohan Kaushal, Ahmad Jamal, Liping Zhang, Bradley K. Taylor and Karin N.

Westlund (2012)

Knockdown of Locus Coeruleus Noradrenergic Neurons Alleviates Chronic

Orofacial Pain

Society for Neuroscience, 2012

COMPUTER SKILLS:

. Graph Pad Prism6, MS Word, Excel, End note, Power Point, Adobe

Photoshop, SPSS

Data analysis

. Well acclimatized with NCBI, PubMed and other Bioinformatics software

viz. RasMol, Cn3D, Swiss-PDB, Clustal W, GenScan and NebCutter,

sequence alignment using BLAST and FASTA

STRENGHTS:

. Expertise in stereotaxic brain surgeries and behavior testing

. Experience in the field of manufacturing of pharmaceuticals in general

and vaccines

. Experience in customer service

. Presentation skills and quick learning capability

PROFESSIONAL SOCIETY MEMBERSHIPS:

2009-Current Member: Bluegrass Chapter of the Society for Neuroscience

(BGSFN), UK

2012 Member: Louisville Chapter-Society for Neuroscience, UL

2012 Member: Society for Neuroscience (SFN)

ADDITIONAL TRAINING:

. Attended 10 day workshop on Immunodiagnostics organized Dr. Subbarao

Gunupuddi, Owner and Director, Dxsys Systems, San Francisco

. Attended 3 week training on "Role of Biotechnology in Aquaculture"

under supervision of Dr. Janardan Jee at National Research Centre for

Makhana, ICAR- Darbhanga, India

REFERENCES

. Available on request

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