JON F. TALLY

*** ** **** ***** ***** Home: 816-***-****

Lee’s Summit, MO 64082 Cell: 816-***-****

acc7tb@r.postjobfree.com

EXECUTIVE SUMMARY:

Jon Tally is a senior scientist with over 25 years experience developing innovative

experimental solutions to explore biochemical properties of proteins. He is proficient

in protein purification in gram quantities, assay development, electron microscopy,

analytical method troubleshooting and documented expertise in protein-ligand

interaction both quantitatively and qualitatively as a purification strategy.

PROFESSIONAL QUALIFICATIONS:

• Skilled protein structure scientist and innovative biochemical analyst.

o Developed new and troubleshooted standard methods for purification of

gram quantities of numerous protein species from a variety of prokaryotic

and eukaryotic organisms.

o Proven experience and aptitude for quantifying protein- ligand

binding kinetics using spectroscopy, microcalorimetry and biolayer

interferometry in multiple protein-ligand systems.

o Trained associates in new techniques of biochemical analysis: high

resolution electron microscopy, protein purification, enzyme kinetics,

UV-Vis and fluorescence spectroscopy, biolayer interferometry and

isothermal titration microcalorimitry.

o Maintained and monitored calibration of laboratory instrumentation and mentored

technicians in proper care and use of analytical instrumentation.

• Recent Scientific achievements

o First to produce and visualize by electron microscopy the formation of

hydrophobic pore forming assemblies of Tetanus neurotoxin inserted into

lipid nanodisks.

o Determined the pH and concentration dependence of bacterial toxin

hydrophilic to hydrophobic transition using bio-layer interferometry.

o Published numerous peer reviewed articles in frontline scientific journals

describing the kinetics of transient substrate species of enzyme reactions.

EDUCATION:

M.S. - Microbiology, University of Missouri Kansas City, Kansas City, MO

B.S. - Biology, University of Missouri Kansas City, Kansas City, MO

AFFILIATIONS:

American Chemical Society (ACS) (2008 – present)

JON F. TALLY

808 SW Lake Pines Drive

816-***-****

Lee’s Summit, MO 64082

acc7tb@r.postjobfree.com

American Association for the Advancement of Science (AAAS) (2005 – present)

EMPLOYMENT HISTORY:

Visiting senior scientist volunteer, UNIVERSITY OF KANSAS MEDICAL

CENTER, Kansas City, KS (April, 2013 – July, 2013)

• Visiting senior scientist exploring protein-ligand binding using biolayer

interferometry.

Senior Research Associate UNIVERSITY OF KANSAS MEDICAL CENTER,

Biochemistry and Molecular Biology, Dr. Mark Fisher Laboratory, Kansas City, KS

(August, 2011 - March, 2013)

• Developed methods for transformation of hydrophilic monomers to hydrophobic

oligomers of the membrane insertable form of Tetanus toxin for ligand binding

studies using biolayer interferometry and high resolution electron microscopy.

• Directed protein purification using a variety of gel chromatography media using

AKTA (GE) and Bio-Rad controllers. Many proteins were used for biochemical

analysis of proteins implicated in protein miss-folding disease and recovery of

proteins trapped in insoluble inclusion bodies.

Senior Research Associate UNIVERSITY OF KANSAS MEDICAL CENTER,

Biochemistry and Molecular Biology, Dr. Harvey Fisher Laboratory, Kansas City, KS

(August 2006 - August 2011)

• Supervised production of numerous cloned enzyme mutants by gel filtration and

affinity chromatography.

• Designed experiments and interpreted the kinetics of deuterium substrate isotope

and solvent isotope effects to elucidate the reaction mechanism of various enzymes.

Managing Stockholder LAKE PINES ASSOCIATES, Lee’s Summit, MO

(January, 2005 - July, 2006)

• Co-owned and operated a Car-X automotive repair franchise during a break of

NIH funding.

Senior Research Associate VETERANS AFFAIRS MEDICAL CENTER and

UNIVERSITY OF KANSAS MEDICAL CENTER, Kansas City, KS (1992 - 2004)

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JON F. TALLY

• Designed experimental procedures and interpreted results of investigations related

to enzyme structure, function and mechanism viewed through UV-Vis

spectroscopy, electron microscopy and micro-calorimetry.

• Purified gram quantities of various dehydrogenase species from anaerobic cell

cultures as well as E.Coli mutants.

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