CURRICULUM VITAE

S/o Shashigangadhar

SHIVAVEERAKUMAR S.

H. No. 10-2/58,

Sangameshwar Colony,

Gulbarga – 585 103

Mobile No.: +91-998*******

+91-984*******

E-mail: acc4w7@r.postjobfree.com

Objective:

To be associated with an Organization/Institution where my knowledge

and skills are utilized and which provides an opportunity to enhance my

knowledge and skills.

ACADEMIC PROFILE:

Thesis on “Production of tyrosinase by actinomycetes and their molecular

characterization with metagenomic attributes” for the award of Doctor of

Philosophy degree on 15.02.2014

During Ph.D.: Selected for CIMO International Research Fellowship

Since Aug. 2011 to Mar. 2012 Working at Department of Environmental Sciences,

Helsinki University, Niemenkatu 73, Lahti – 14150, Finland

Ph. D. in Microbiology (Registered in 2008)

Registration in 2008

Department of Microbiology, Gulbarga University, Gulbarga.

Thesis Entitled: “Production of tyrosinase by actinomycetes and their molecular

characterization with metagenomic attributes”

Research Guide: Dr. Dayanand Agsar, Professor of Microbiology.

M. Sc. in Microbiology (2005-2007)

Department of Microbiology, Gulbarga University, Gulbarga.

Class: 67.75% Aggregate

B. Sc. Integrated Biotechnology (2002-2005)

Vishveshwariah College of Applied Science, Gulbarga.

Class: 78.92% Aggregate.

RESEARCH AND TEACHING PROFILE:

RESEARCH EXPERIENCE (5 years):

Completed project “‘Recent trends in actinobacterial diversity of compost

and their potential bioactive molecules’ with 16S rDNA and Metagenomic

approach by Pyrosequencing” funded by TEKES and National institute of

occupational health and Centre for International Mobility and cooperation,

Finland from August, 2011 to February, 2012.

Completed thesis of Ph.D. programme entitled: “Production of tyrosinase

by actinomycetes and their molecular characterization with metagenomic

attributes” under the guidance of Dr. Dayanand Agsar, Professor of

Microbiology Gulbarga University, Gulbarga since from May, 2008 to till

the date.

Completed project “Isolation, antibiogram and plasmid curing of

aetiological agents of urinary tract infection” under the guidance of Dr.

C. T. Shivannavar, Professor of Microbiology, Gulbarga University,

Gulbarga from April, 2007 to October, 2007.

Completed project on “Detection of Potential Proteolytic Microorganisms”

under the guidance of Dr. Sarvamangala R. Patil, Vishveshwariah College

of Applied Science, Gulbarga from March, 2004 to September, 2004.

TEACHING EXPERIENCE (2 years):

Since from May, 2008 involved in academic activities as theory and practical

classes for the Post Graduate (Microbiology) studies.

PAPERS ACCEPTED/ PUBLISHED:

S. Shivaveerakumar, D.N. Madhusudhan, H. Raghavendra, Dayanand

agsar, Screening of Streptomyces and process optimization for the

production of tyrosinase. Journal of Pure and Applied Microbiology. Vol 8,

No 8, 2013 (Accepted) (Impact factor: 0.065)

Mousumi das, S. Shivaveerakumar, Dayanand agsar, Extracellular

production of L-Glutaminase under submerged system by Streptomyces

rochei detected from the lime stone quarries of deccan trap. Journal of Pure

and Applied Microbiology (2013, Accepted) (Impact factor: 0.065)

S. Shivaveerakumar, D.N.Madhusudhan, Dayanand agsar, Merja Kontro,

Catalytic zone, a novel screening approach for the extracellular tyrosinase

by Streptomyces vinaceusdrappus DSV5. Journal of Pure and Applied

Microbiology. Vol 7, No.4, 2013 (Accepted) (Impact factor: 0.065)

“Production of L-Glutaminase by Pseudomonas VJ-6”. Hiremath Jyothi,

Shivaveerakumar, Rathod Vandana, Research Journal of Biotechnology, 42

– 49, Vol 6(3), August 2011 (Impact factor: 0.143)

CONFERENCES/WORKSHOP/SEMINAR PARTICIPATED

Participated in two days KSTA regional conference on Science and

technology for development organized by Central University of Karnataka,

School of Earth Sciences, on 30th and 31st January 2014.

Presented a poster in International conference on Advances in

Biotechnology and Bioinformatics entitled “Evaluation of upstream

bioprocessfor the enhanced production of tyrosinase by Streptomyces

vinceudrauppus” held at Dr. D.Y. Patil Vidyapeeth Pune from Nov. 25-27,

2013.

Participated in the National conference on Nanotechnology’s Invisible

Threat: Small Science, Big Consequences” organized by Mahatma Gandhi

National Institute of Research and Social Action (MGNIRSA), Hyderabad

from 26-27, September, 2013.

Attended one day seminar on Food Security and Natural Resources

Management sponsored by University Grants commission New Delhi,

organized by Faculty of Science and Technology Gulbarga University,

Gulbarga on 8th October, 2012.

Attended two days workshop on Research Methodology for Life Science

Research Students organized by Department of Biotechnology, Gulbarga

University, Gulbarga from 29th –30th May, 2012

Presented a poster in First National Conference on Recent Advances in

Nanobiotechnology entitled “Protoplast fusion in Streptomyces for

enhanced production of L-Glutaminase and Tyrosinase” held at Post

Graduate Department of Studies and Research in Biotechnology, Gulbarga

University, Gulbarga from 20th to 21th March, 2012

Participated in National Seminar on Environment Education for Sustainable

Development organized by Purushottam Walwalkar Higher Secondary

School, Mapusa in collaboration with Centre for Environment Education,

Goa from 25th to 27th November, 2010.

Participated in State Inter University Symposium (Biochemistry,

Biotechnology, Microbiology and Bioinformatics) sponsored by Vision

Group on Science and Technology, Department of Science and Technology,

Government of Karnataka, held at Gulbarga University, Gulbarga on 3rd

and 4th March, 2010.

Attended National Symposium on “Role of Biology and Biotechnology in

Conservation of Biodiversity and Sustainable Development” held at

Department of Botony, Gulbarga University, Gulbarga from 22nd – 24th

December 2009.

Presented a poster entitled “Production of Tyrosinase by novel isolates of

Streptomyces” organized by 50th Annunal Conference Association of

Microbiologist of India held at National Chemical Laboratory, Pune from

15th to 18th December 2009.

Attended seminar on “Advances in Nanotechnology” organized by

Vishweshwariah College of Applied Sciences, Gulbarga, Nov, 2009.

Attended second National conference on Science and technology organized

by Karnataka Science and Technology Academy and Gulbarga University

Gulbarga on Sept 23 – 25, 2009.

Participated in symposium “Antimicrobial Resistance” organized by

Sir Dorabji Tata Centre for Research in Tropical Diseases, held at

Department of Microbiology and Cell Biology, Indian Institute of Science,

Bangalore on March 10 – 11, 2008.

Presented a poster entitled “Isolation, antibiogram and plasmid curing of

aetiological agents of urinary tract infection” organized by 2nd National

Conference on Biotechnology for industrial and rural development

(NCBIRD 2008) held at Department of Biotechnology, Gulbarga University,

Gulbarga from 17th to 19th January 2008.

Attended the One Day Awareness Training Programme on Biodiversity

Related Issues and People’s Biodiversity Register, organized by Department

of Biotechnology, Gulbarga University, Gulbarga and Karnataka

Biodiversity Board, Bangalore held at Department of Biotechnology,

Gulbarga University, Gulbarga from 21st September 2007.

Participated One Day Seminar on Genomics and Biotherapy at

Vishweshwariah College of Applied Sciences, Gulbarga on 19th April 2007.

Attended the workshop on Intellectual Property Rights organized by the

Department of Law, Gulbarga University, Gulbarga and Centre for

Intellectual Property Rights, Research and Advocacy (CIPRA), National

Law School of India University (NLSIU), Bangalore held at Gulbarga

University, Gulbarga on February 28th – 1st March 2005.

TRAINING PROGRAMMES ATTENDED:

Successfully completed one month Crash course on Bioinformatics

conducted by Gulbarga University Gulbarga on March 1st - 31st, 2009.

Successfully completed Diploma in Advanced English conducted by Oxford

English Academy, Gulbarga on 10th June - 10th August 2009.

Successfully completed the course in Techniques in Molecular Biology

organized by Central Food Technological Research Institute held at CFTRI,

Mysore from 5th – 16th June 2006.

Participated in one day training programme on “In Vitro Fertilization”

organized by Centre for Infertility Management, Hyderabad held at MHRI

Centre for Infertility Management, Hyderabad on 1st October 2004.

MY CREDENTIALS:

Secured Second Prize in Mime held at Jnana Ganga Campus, Gulbarga

University, Gulbarga during Inter Collegiate Youth Festival from

26th – 28th November, 2004.

HIGHLIGHTS OF PRESENT RESEARCH WORK:

(Ph. D.): Research work done at Gulbarga University

Collection of samples from unique, extreme and harsh ecosystems.

Pre treatment and enrichment of samples.

Isolation of Streptomyces from the pretreated samples.

Cultural characteristic of streptomyces.

Physiological and biochemical characterization of Streptomyces on different

media.

Screening of streptomyces for bioactive molecules (Tyrosinase).

Media formulation and process standardization for production of specific

bioactive molecules (Tyrosinase).

Effect of physicochemical parameters on tyrosinase production.

Isolation and purification of tyrosinase.

Stability and kinetic studies of tyrosinase.

Preliminary studies on the, possible molecular aspects of tyrosinase.

Purified analytical tyrosinase is used for the fabrication of tyrosinase

biosensor to detect the phenolic compounds.

16s rDNA sequencing of potential isolates of Streptomyces.

Sequence analysis using Bioinformatics tools.

Blast, Fasta for the analyzed sequences to characterize up to their species

level.

(Ph. D.): Research work done at University of Helsinki

Collection of compost samples from various facilities

Isolation and screening of isolates for the production of bioactive molecules

Characterization of the potential isolates by 16SrDNA sequencing

(Ph. D.): Research work done at University of Helsinki on Pyrosequencing

Isolation of direct DNA from different compost samples

Polymerase chain reaction of the DNA by actinobacterial primers

Sequencing of the product after PCR for metagenomics studies

Analysis of metagenomics sequences to evaluate the diversity of the

actinobacteria in compost

Future approaches:

Analysis of the metagenomic sequences from Finland

TECHNIQUES KNOWN

Microbial techniques

Isolation and maintenance of microorganisms – Bacteria, Fungi,

Actinomycetes and bacteriophages.

Microscopic studies by light microscope.

Antimicrobial susceptibility test by Kirby-Bauer’s disk method and well

method.

Antibiogram

Production of Alcohol, Wine, Yoghurt, Mushrooms etc.

Assessment of quality of water (DO, BOD, COD, MPN) and milk (MBRT,

SPC, DMC).

Production of biofertilizers (Rhizobium, Azotobacter, Azospirillum, VAM, etc.)

Molecular Biology:

PCR and DNA Sequencing

Sequence Analysis

Isolation of DNA from plant and microbial source.

Isolation of plasmid DNA, Plasmid curing

Isolation of drug resistant bacteria

Agarose Gel Electrophoresis

SDS-PAGE

Restriction digestion

Metagenomics of actinobacteria

Biochemistry and Enzymology

Qualitative analysis of biomolecules (Carbohydrates, proteins, lipids,

nucleic acids etc.)

Isolation and optimization parameters studies of enzymes

(Amylase,Protease, Cellulase, Pectinase, Asparaginase).

Paper, thin layer chromatography.

Purification of Enzyme.

Fatty acid analyses of Actinobacteria

Bioinformatics and software’s:

MS-DOS, MS-Office, C++ language, Internet Knowledge

Accessing information from biological information providers (EMBL, NCBI,

PIR, PDB, GenBank Etc.)

Blastn, Clustalw, Clustalx, Fasta, Rasmol, Prime 3, Chroma, Phylip,

tree viewX, Oligo.

Instruments handled:

Camera Microscope(Nikon 32)

pH meter, BOD incubator

Electrophoresis

UV-VIS spectrophotometer

Ultra Cold centrifuge, Desktop centrifuge.

Agarose Gel Electrophoretic unit, SDS-PAGE unit, Restriction digestion kit.

PERSONAL STRENGTHS:

Independently capable of handling relevant Bioinformatics softwares, a

quick and efficient learner having a strong belief and confidence. I am also

capable of taking fast independent decision; I can also work in a team and

always an open minded researcher.

MY INTEREST:

Actinomycetes and their Bioactive molecules.

Interested in advanced techniques in Molecular and Biochemical systematic

studies such as 16s rDNA, Gene sequencing, Chemo systematic analysis of

actinobacteria and tyrosinase Enzyme engineering studies.

BIOGRAPHICAL DETAILS:

Father's Name : Shashigangadhar S.

Date of Birth : 10th October 1984

Sex : Male

Languages Known : English, Hindi, Kannada and Telugu

Corresponding Address : Department of Microbiology,

Gulbarga University,

Gulbarga – 585 106

Nationality : Indian

Religion : Hindu

Marital Status : Married

REFERENCE

Dr. Dayanand Agsar Dr. Merja Kontro

Professor Associate Professor

Department of Microbiology, Department of Environmental

Gulbarga University, Sciences

Gulbarga - 585 106 Helsinki University,

Karnataka - India. Niemenkatu 73, Lahti

Ph. No. 0091-8472-247180 (O) Finland- 14150

Mobile No.: +91-944******* Mobile No.: +358-*********

E-mail: acc4w7@r.postjobfree.com E-mail: acc4w7@r.postjobfree.com

Declaration:

I hereby declare that the information furnished here is correct and true to

the best of my knowledge.

Place: Gulbarga, India

Date: 18.02.2014 (Shivaveerakumar S.)

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