SAAYI KRUSHNA

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Objective:

I'm qualified, competent, forward thinking and committed research

scientist with a proven ability to implement new methods and

standardization of protocols and successful in leading the academic

research projects, now seeking a challenging position in an industry to

expand my research skills and to nurture the leadership skills.

Research experience:

Organization: Northwestern University

Role: Research Associate

Tenure: 2011- to date

Responsibilities:

< To find the role of Adenoviral protein E1A binding to cellular protein

p300 in induction of DNA replication and DNA damage response.

< Studying the gene expression changes in the cell due to expression of

Adenoviral protein E1A

< To understand the E1A oncogene induced rereplication of cellular DNA and

altered DNA replication dynamics.

< Represented the data in various scientific meetings and journal clubs.

< Trained graduate students in their projects and provided hands on

experience to handle various analytical instruments

< Lab management

< Data review and preparation of scientific papers to communicate to

publication.

Organization: Loyola University Medical Center

Role: Research Associate

Tenure: 2010- 2011

Responsibilities:

< Involved in a project where natural and recombinant hirudin antibodies

were evaluated for cross reactivity.

< Responsible for protein profiling of various clinical samples using

SELDI-TOF-MS.

< Actively involved in the quality assurance of various heparin compounds

using HPLC.

< Provided hands on experience to under graduate and graduate students and

mentoring their research projects.

< SOP writing, implementation and data interpretation.

< Data review and preparation of manuscript for publication.

< Review of literature and writing project proposals.

Organization: R&D center, Dayananda Sagar Institutions,

India

Role: Head, Department of Biochemistry

Tenure: 2002- 2003; 2006-2010.

Responsibilities:

< Mentoring student project and data review

< Lab management

< Analytical training

< Writing and implementing SOPs

< Manuscript preparation and data presentation

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Education:

. Ph.D in Biochemistry, Sri Krishnadevaraya.University, India

2010

. M.Phil in Biochemistry, Sri Krishnadevaraya.University, India

2006

. MS in Biochemistry, Madras University, India

2002

. BS in Biological Sciences, Sri Venkateshwara University, India

1998

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Research techniques:

> Basic Microbiology and cell culture techniques (BSL-2 environment)

> Molecular Biology

. Isolation of DNA, RNA and genomic DNA isolation

. Electrophoresis techniques: SDS-PAGE, Agarose Gel Electrophoresis

. Southern Blotting, Western Blotting.

. PCR, RT-PCR

. Cloning and gene expression

. shRNA construction and gene regulation

.

> Immunological and Biochemical

. Flowcytometry (Cell cycle analysis; apoptosis), Enzyme linked

immunosorbent assay (ELISA), Immunoprecipitation,

immunofluorescence.

> Cell and tissue culture

Primary cell culture (Keratinocytes isolation and culture),

secondary mammalian cell cultures, stable cell clines

preparation.

> Transfection and Transduction

> Analytical techniques

. HPLC

. SELDI-TOF-MS

> Animal handling (Rats)

Dissections, blood collection, and IP injections.

Publications:

* Ghata Singhal, Elisabetta Leo, Saayi Krushna Gadham Setty, Yves

Pommier, Bayar Thimmapaya. Adenovirus E1A oncogene induces

rereplication of cellular DNA and alters DNA replication dynamics. J.

Virology, August 2013; 87(15): 8767-8778.

* Abdul Kareem M, Saayi Krushna G, Shaik AH, Maruthi Prasad E and

Lakshmi Devi K. Protective effect of Nutmeg aqueous extract against

experimentally induced hepatotoxicity and oxidative stress in rats.

J. Ayurveda & Integrative Medicine, 2013; 4(4): 216-223.

* G. Saayi Krushna, M. Abdul Kareem, V. Damodara Reddy, P. Padmavathi,

S. Altaf hussain and Lakshmi Devi K. Aegle marmelos fruit extract

attenuates isoproterenol-induced oxidative stress in rats. J Clin

Biochem Nut, May 2012; 50(3): 199-204.

* Nasir S, Zhu H, Saayi Krushna G, Josephine C, Hoppensteadt D and

Fareed J. " Cross-reactivity of varius thrombin products with anti-

rabbit antibodies to bovine, human and recombinant thrombin. Clinical

and Applied Thrombosis/Hemostasis, 2012, 18(3): 243-248.

* Hussain Shaik A, Rasool SN, Abdul Kareem M, Krushna GS, Akhtar PM,

Devi KL. Maslinic acid protects against Isoproterenol-induced

cardiotoxicity in albino Wistar rats. J Med Food, 2012, 15(8): 741-

746.

* Shaik AH, Rasool SN, Vikram Kumar Reddy A, Abdul Kareem M, Saayi

Krushna G, Lakshmi Devi K. Cardioprotective effect of HPLC

standardized ethanolic extract of Terminalia pallida fruits against

Isoproterenol-induced myocardial infarction in albino rats. J

Ethnopharmacol, 2012, May7; 141(1): 33-40.

* G. Saayi Krushna, M. Abdul Kareem, K. Nagamani and K. Lakshmi Devi.

Preventive effect of Aegle marmelos fruit extract on Isoproterenol

induced atherogenesis in rats. Int. J. Pharm. and Technol., 2011, 3

(1): 1934-1945.

* G. Saayi Krushna, M Abdul Kareem and K Lakshmi Devi.

Antidyslipidaemic effect of Aegle marmelos Linn. fruit on

Isoproterenol induced myocardial injury in rats. The Internet Journal

of Pharmacology, 2009, 6(2): (Online).

* M. Abdul Kareem, G. Saayi Krushna, S. Altaf hussain and K.

Lakshmidevi. "Effect of Nutmeg aqueous extract on hyperglycaemia,

hyperlipidaemia and cardiac histology associated with isoproterenol-

induced myocardial infarction in rats". Tropical Journal of

Pharmaceutical Research, 2009, 8(4): 337-344.

* G. Saayi krushna, M. Abdul Kareem and K. Lakshmi Devi.

"Cardioprotective and antilipid peroxidative effect of Aegle marmelos

against isoproterenol induced cardiac stress in rats". International

journal of green pharmacy, 2007, 1(3-4): 13-15, Oct.-Dec.

* Damodara Reddy.V, Saayi Krushna G, Padmavathi. P and Varadacharyulu.

N.Ch. "Effect of Emblica officinalis against alcohol-induced

biochemical changes in plasma and red blood cells of rats". African

Journal of Biochemistry Research, 2007, 1(6): 101-105.

Phone: 1-847-***-****

E-mail: acc44x@r.postjobfree.com

1569 S WOLF ROAD APT#103

PROSPECT HEIGHTS

IL-60070

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