Padmaja Gade, Ph.D

acba7c@r.postjobfree.com

****, ******* ***** ***** • Ellicott city, MD 21043 • 443-***-****

Education

Hyderabad Central University, Department of Biochemistry, India

• Ph.D. in Biochemistry, 2006

• Council of Scientific and Industrial Research Fellowship

Hyderabad Central University, Biotechnology, India

• Masters in Biotechnology, 2000

Nagarjuna University, India

• Bachelors in Microbiology and Biochemistry, 1998

Visa Status: Permanent resident (Green card holder)

Research Experience

University of Maryland, Baltimore, MD

Research Associate, School of Medicine, Oncology 2011-present

• A connection between ER stress and autophagy of an IFN-induced regulation of anti-metastatic gene.

University of Maryland, Baltimore, MD

Post-doctoral fellow, School of Medicine, Oncology 2006-2011

• Regulation of novel IFN-induced cell death activating genes.

Hyderabad Central University, India

Ph.D Candidate 2000-2006

• Identification and Characterization of Topoisomerase II alpha and beta kinase activity in HIV-1 infected cells and virus particles.

Hyderabad Central University, India

Summer trainee, 1999

• Established method of PCR screening for Chlamydial infection in patients.

Skills and Techniques

Employed all standard molecular biological techniques including cloning, mammalian cell culture, drug assays, transfection, mutational analysis, gene expression analysis, promoter assays, expression of recombinant proteins, protein:protein interactions, autophagy and apoptosis assays, DNA binding assays such as ChIP assays, Q-PCR analysis, RT-PCR, RNAi, reconstitution of gene expression in mammalian cells following RNAI, Lentiviral gene expression systems, knock out cell lines and knockout mice, induction of tumor carcinogenesis in mice, infection models, kinase assays, Immuno assays, protein purification.

Positions and Employment

2011 – Till Date Research Associate, Cancer Center, School of Medicine, University of Maryland, MD

2006-2011 Post-Doctoral fellow, Cancer Center, School of Medicine, University of Maryland, MD

Other Experience and Professional Memberships

2010 – 2012 Member of International Society for Interferon and Cytokine Research

2010 Member of American Society of Biochemistry and Molecular biology

2007 Member of American Society of Biochemistry and Molecular biology

Honors

2011 Reviewer of Cell Biology International.

2010 Recipient of Outstanding Postdoctoral Award for oral presentation,

Annual Cancer Biology Research Retreat Univ of Maryland, Biopark.

2010 Recipient of ASBMB Postdoctoral travel award

2010 Recipient of ISICR Postdoctoral travel award

2007 Recipient of ASBMB Postdoctoral travel award

2003-2005 Senior research fellowship grant by Council of Scientific and Industrial Research, New Delhi, India.

2001-2003 Junior research fellowship grant by Council of Scientific and Industrial Research,New Delhi, India.

2000 All India Biochemistry entrance examination rank 1 for Ph.D.

1998 All India Biotech entrance exam rank 9th for Masters.

Peer-reviewed Publications

1. Gade, P., Ramachandran, G., Maachani, U.B., Rizzo, M.A., Okada, T., Prywes, R., Cross, A.S.,

Mori, K. and Kalvakolanu, D.V. (2012). An IFN-gamma-stimulated ATF6-C/EBP-beta-signaling

pathway critical for the expression of Death Associated Protein Kinase 1 and induction of

autophagy. Proc Natl Acad Sci U S A 109: 103**-*****.

2. Gade, P., Roy, S.K., Li, H., Nallar, S.C., and Kalvakolanu, D.V. (2008). Critical role for

transcription factor C/EBP-beta in regulating the expression of death-associated protein kinase 1.

Mol Cell Biol 28:2528-48.

3. Gade, P., Singh, A. K., Roy, S. K., Reddy, S. P., and Kalvakolanu. D.V. (2009). Down-regulation

of the transcriptional mediator subunit Med1 contributes to the loss of expression of metastasisassociated dapk1 in human cancers and cancer cells. Int J Cancer 125:1566-74.

4. Kalvakolanu D.V., Gade P. (2012). IFNG and autophagy: A critical role for the ER-stress mediator

ATF6 in controlling bacterial infections. Autophagy 8.

5. Li, H., Gade, P., Nallar, S. C., Raha, A., Roy, S. K., Karra, S., Reddy, J. K., Reddy, S. P., and

Kalvakolanu, D. V. (2008). The Med1 subunit of transcriptional mediator plays a central role in

regulating CCAAT/enhancer-binding protein-beta-driven transcription in response to interferon gamma.

J Biol Chem 283, 130**-*****.

6. Shen, F., Li, N., Gade, P., Kalvakolanu, D. V., Weibley, T., Doble, B., Woodgett, J. R., Wood, T.

D., and Gaffen, S. L. (2009). IL-17 receptor signaling inhibits C/EBPbeta by sequential

phosphorylation of the regulatory 2 domain. Sci Signal 2, ra8.

7. Sikder, H., Zhao, Y., Balato, A., Chapoval, A., Fishelevich, R., Gade, P., Singh, I. S., Kalvakolanu,

D. V., Johnson, P. F., and Gaspari, A. A. (2009). A central role for transcription factor C/EBP-beta

in regulating CD1d gene expression in human keratinocytes. J Immunol 183, 1657-1666.

8. Kondapi, A. K., Padmaja, G., Satyanarayana, N., Mukhopadyaya, R., and Reitz, M. S. (2005). A

biochemical analysis of topoisomerase II alpha and beta kinase activity found in HIV-1 infected

cells and virus. Arch Biochem Biophys 441, 41-55.

9. Nallar, S. C., Lin, L., Srivastava, V., Gade, P., Hofmann, E.R., Reddy, S.P., Kalvakolanu, D.V.

(2011). GRIM-1, a Novel Growth Suppressor, Inhibits rRNA Maturation by Suppressing Small

Nucleolar RNAs. PLoS One 6: e24082.

10. Shanmugam, R., Gade, P., Weekes, A.W., Sayar, H., Suvannasankha, A., Goswami, C., Li, L.,

Gupta, S., Cardoso, A.A., Baghdadi, T, Al., Sargent, K.J., Cripe, L.D., Kalvakolanu, D.V., Boswell,

H.S. (2011) A non-canonical Flt3ITD/NF-κB signaling pathway represses DAPK1 in acute myeloid

leukemia (AML) Clin Cancer Res 18: 360-369.

Review Article

Li, H., Gade, P., Xiao, W., and Kalvakolanu, D.V. 2007. The interferon signaling network and

transcription factor C/EBP-beta. Cell Mol Immunol 4:407-18.

Book Chapters

1. Gade, P., and Kalvakolanu, D.V. (2010). Chromatin immuno-precipitation (ChIP) Assay as tool for

analyzing transcription factor activity. Methods in Molecular Biology 809: DOI 10.1007/978-1-

61779-376-9_6.

2. Gade, P., and Kalvakolanu, D.V. (2014). Death associated protein kinase 1 suppresses tumor

growth and metastasis via autophagy and apoptosis. Autophagy. Chapter 19. Pages 277-292.

Abstracts and Oral presentations at professional meetings

1. Gade, P., and Kalvakolanu, D.V. (2010). Transcriptional mediator subunit Med1 is required for the

expression of metastasis-associated gene, The death associated protein kinase-1 (DAPK1)

FASEB J 24:676.1. (Poster)

2. Gade, P., and Kalvakolanu, D.V. (2010). Antimetastatic effects of Interferons: Role of DAPK1 and

its regulators. ISICR meeting at Chicago (Oral)

3. Gade, P., Li, H., Roy S. K., and Kalvakolanu, D. V. (2007). Transcription factor C/EBP-beta is

required for the regulation of apoptotic gene, the death-associated protein kinase-1 (DAPK1)

FASEB J 21:A251. (Poster)

4. Li, H., Gade, P., and Kalvakolanu, D. V. (2007). TRAP220 interacts with CCAAT/enhancerbinding

protein-beta and regulates IFN-induced gene transcription FASEB J 21 :A251 (Poster)

5. Gade, P., Roy S. K., and Kalvakolanu, D. V. (2007). A Critical Role for Transcription Factor

C/EBP-beta In Regulating the Interferon Induced Expression of the Metastasis Regulatory Gene,

Death associated Protein Kinase-1. ISICR meeting at Oxford, United Kingdom. (Oral)

6. Nallar, S. C., Lin. L Gade, P., Hofmann, E. R., and Kalvakolanu, D. V. (2009). A novel small RNA

regulatory mechanism employed by Interferons for regulating growth. ISICR meeting at Lisbon.

(Poster)

7. Gade, P., and Kalvakolanu, D. V. 2010. Transcriptional mediator subunit Med1 is required for the

expression of metastasis-associated gene, the death associated protein kinase-1 (DAPK1)

Annual Page4 of 4 Cancer Biology Retreat, Univ of Maryland (Oral).

References upon request.

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