Boris Sabirzhanov, Ph.D.

Phone: 605-***-****; email: acb5yq@r.postjobfree.com

D.O.B: 12/26/1976; VISA Status: Permanent resident (green card) ; Availability: 02/25/2014

Objective

I am looking for scientist position in R&D environment that requires a strong background in

molecular biology, cell biology and neuroscience

Summary

Ph.D. in Molecular biology with experience in molecular and cell biology, neuroscience,

neurodegenerative diseases and epigenetics

Accomplishments

Successfully completed ten research projects

Proposed and managed four projects

Conducted research in three collaborative projects

Proposed and developed method for screening dsRNAs for potential off-target effects

Author of more than twenty original research manuscripts

Awarded five grants and awards

Experience

2010-present Postdoctoral fellow

School of Medicine, University of Maryland (P.I.: Dr. Alan Faden)

Project: Molecular mechanisms of cell death and inflammation after traumatic brain injury

Results:

Demonstrated neuroprotective effect of HSP70 and its pharmacological inducers

Characterized microRNAs involved in neuronal cell death and neuroinflammation after

traumatic brain injury

Demonstrated neuroprotective effect of microRNA-23a and -27a

Identified potential microRNA markers of microglia activation and neurodegeneration

Detected genes involved in neuroinflammation after traumatic brain injury

Characterized age-related changes in microglia activation and inflammation after brain injury

2004-2010 Postdoctoral fellow

School of Medicine, University of South Dakota (P.I.: Dr. Timothy Clark - deceased)

Project: Molecular mechanisms of learning and memory formation

Results:

Proposed and developed method for screening target specificity of siRNAs for potential off-

target effects.

Identified turtle glutamate receptor subunit 4 (GluA4), and its transcript variants. An alyzed

role of GluA4 splicing variants in AMPA receptor trafficking.

Described turtle BDNF. Characterized changes in DNA methylation profile of BDNF gene

during learning process

Characterized turtle Tolloid-like gene. Demonstrated that tTLL regulates memory formation

and neurodegeneration by cleavage of BDNF precursor and oligomeric Amyloid-β.

Obtained three Pilot Research Project Awards from Center of Biomedical Research

Excellence (COBRE) on Neural Mechanisms of Adaptive Behavior (Grant P20 RR-015567).

Demonstrated activation of the Tolloid-like 1 gene expression under hypoxic conditions.

Trained the members of group in molecular biology methods

BORIS SABIRZHANOV, PhD page 1 of 4

1999 Intern

R&D Department, Immunopreparat, Ufa, Russian Federation

Project: Developing of DNA vaccine for Puumala virus

Assisted in RNA isolation, cDNA syntheses and cloning of Puumala hantavirus nucleocapsid

protein gene

Areas of experience

Molecular biology, cell biology, biotechnology and biochemistry methods

Technical problem solving

in vitro screening of genes and drugs

Upstream process development

Development of molecular and cell-based bioassays

Design, optimization and troubleshooting of protocols

Assays and methods development

Planning and performing of experiments

Data collection, analysis and interpretation

Project design and management

Working on collaborative projects

Preparation of grant proposals, manuscripts, reports, and presentation

Supervising junior members of laboratory

Training colleagues in molecular biology methods

Professional skill

DNA: DNA purification, DNA gel electrophoresis, restriction analysis of DNA, Southern

blotting, DNA sequencing, preparation of radiolabeled DNA probes, single-strand conformation

polymorphism analysis; genome walking

RNA: RNA isolation, quantification and quality control of RNA, cDNA synthesis, Northern blot

RNAi: Design and validation of siRNA; construction of siRNA rescue plasmid

microRNA: isolation, profiling of miRs expression, functional analysis, mRNA targets

prediction

PCR: PCR, RT-PCR, rapid amplification of cDNA ends, real-time PCR; design and validation

of primers and real-time PCR assays; PCR diagnostic of infections

Constructing of recombinant DNA molecules: Cloning of PCR products and DNA fragments

into plasmid vectors; Construction and evaluation of vectors for gene expression into E. coli,

yeast and eukaryotic cell lines and primary cell cultures; Site directed mutagenesis

Proteins: BCA, SDS PAGE, Western blotting; ELISA; Production of recombinant proteins in E.

coli, yeast and eukaryotic cell lines, cell free in vitro protein expression; Protein purification,

affinity chromatography, tandem affinity purification, immunoprecipitation; Enzyme activity

analysis

Cell culture: cell culture media preparation and optimization, preparation of primary neuronal

and microglia cultures, manipulation with variety of cell lines, including neuronal cell lines;

transfection of cell cultures with plasmids, stable cell line generation, siRNA and microRNA

mimics; in vitro cell-based bioassays: Lactate Dehydrogenase, calcein, MTT, caspase-3 activity

Propidium Iodide uptake and Nitric Oxide assays; immunostaining; microscopy

Upstream process development: Cloning of target gene; construction of vectors for gene

expression into E. coli, yeast and eukaryotic cell lines; Site directed mutagenesis and

construction of fused genes; Clone and strain selection; Expression optimization; Development

of strains with stable expression levels

BORIS SABIRZHANOV, PhD page 2 of 4

Production of viral particles: Adeno-associated virus (AAV); lentiviruses; bacteriophage

lambda and bacteriophage M13; Design and construction of AAV and lentiviral vectors for gene

and miR delivery.

Epigenetics: Chromatin immunoprecipitation (ChIP); DNA methylation analysis by bisulfite

genomic sequencing and qPCR

In vivo: Mice handling; genotyping; transcardial perfusion; brain dissection

Bioinformatics: Analysis of nucleotide and amino acid sequences, sequence alignment and

assembly; Design PCR primers and probes for sequenced and non-sequenced species; Analysis

of microarray data; Function, pathway and network analysis of complex data by Ingenuity

Systems (IPA) application

Computer skills: Microsoft Office; Photoshop; sequence analysis: Lasergene (DNASTAR) and

online tools such as NCBI, EMBL; pathway analysis: Ingenuity Pathway Analysis (IPA); gene

expression analysis: GeneSpring GX; statistical analysis: GraphPad Prism, SigmaStat.

Education

1999-2003 Ph.D. in Molecular Biology, Institute of Biochemistry and Genetics, Ufa

Research Center, Russian Academy of Sciences, Ufa, Russia

1994-1999 Master’s degree in Biochemistry, Bashkir State University, Ufa, Russia

Grants and Awards

2009 Pilot Research Project Award from Center of Biomedical Research Excellence (COBRE)

on Neural Mechanisms of Adaptive Behavior. “Analysis the DNA methylation status of

turtle BDNF gene during in vitro classical conditioning”

2007 Pilot Research Project Award from COBRE on Neural Mechanisms of Adaptive

Behavior. “Identification of protein interactions with turtle Tolloid -like protein”

2006 Pilot Research Project Award from COBRE on Neural Mechanisms of Adaptive

Behavior. “Characterization of Tolloid-like protein in reptile and its role in synaptic

plasticity”

2003 Grant of Russian Foundation for basic research (Grant # 03-04-06733МАС)

2002 Grant of Russian Foundation for basic research (Grant # 02-04-07578МАС)

Patents

Use of miR-23a-3p and miR-27a-3p Mimics as Therapeutic Agents for Inhibition of Neuronal

Apoptosis Following Brain Injury (co-inventor) - US Patent Pending UMB Docket: AF-2014-

011

Professional and Editorial Activities

2012 Journal Reviewer, Cellular and Molecular Neurobiology

2012 Journal Reviewer, Pharmacological Research

2010 Journal Reviewer, Annals of Applied Biology

2009 Journal Reviewer, Genetika Russian

2008 Journal Reviewer, Mol Biol (Mosk) Russian

2007 Journal Reviewer, Prikl. Biokhim. Mikrobiol. Russian

BORIS SABIRZHANOV, PhD page 3 of 4

References:

Alan I Faden, M.D.

Professor, Departments of Anesthesiology, Anatomy & Neurobiology, Neurosurgery, and

Neurology

Director, Center for Shock, Trauma & Anesthesiology Research (STAR)

University of Maryland School of Medicine

20 Penn Street (HSFII), #S247

Baltimore, MD 21201

Phone: 410-***-****

E-mail: acb5yq@r.postjobfree.com

Junfang Wu, B.M., Ph.D.

Assistant Professor, Shock, Trauma and Anesthesiology Research (STAR) Center, Department

of Anesthesiology, School of Medicine, University of Maryland, Baltimore

655 West Baltimore Street

Baltimore, Maryland 21201

Phone: 410-***-****

E-mail: acb5yq@r.postjobfree.com

Bogdan Stoica, PhD.

Assistant Professor, Shock, Trauma and Anesthesiology Research (STAR) Center, Department

of Anesthesiology, School of Medicine, University of Maryland, Baltimore

655 West Baltimore Street

Baltimore, Maryland 21201

Phone: 410-***-****

E-mail: acb5yq@r.postjobfree.com

David Swanson, PhD.

Professor, Department of Biology, University of South Dakota

414 East Clark Street

Vermillion, SD 57069

Phone: 605-***-****

E-mail: acb5yq@r.postjobfree.com

Cliff H. Summers, PhD.

Professor, Department of Biology, University of South Dakota

414 East Clark Street

Vermillion, SD 57069

Phone: 605-***-****

E-mail: acb5yq@r.postjobfree.com

I will be happy to show Dr. Timothy G. Clark as my reference, but he died as a result of a car

accident.

BORIS SABIRZHANOV, PhD page 4 of 4

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