Hongjie Pan, PhD

*** ***** ***** **

Chapel Hill, NC27514

Email: acb577@r.postjobfree.com

Tel: 919-***-****

QUALIFICTION

Experience in innate and acquired immunity by using in vivo and in

vitro mouse model.

Expertise in multiple-color Flow Cytometry, cell-based immune

assays, ELISA, Elispot, qRT-PCR, western blotting, cloning and vector

design, and so son

Experience in utilization of the small molecules to block the certain

signal pathways in vivo and in vitro studies.

Expertise in histopathololgy

Total of 18 the first author (9) or co-coauthors (9) peer-reviewed papers

published in immunology or cell biology Journals

EDUCATION

The University of Georgia 1998---2002

• PhD, Department of Pathology, College of veterinary medicine,

Athens, GA 30602, USA.

Nanjing Agricultural University 1987---1990

Master of Science (MS), Veterinary Parasitology,

•

College of veterinary medicine, Nanjing, China.

AnHui Agricultural University 1983---1987

• Bachelor of Science (DVM), Veterinary Medicine,

AnHui Agricultural University, Hefei, China.

PROFESSINAL SKILLS

Multiple-color Flow Cytometry analysis (FACS), PBMC tetramer

•

staining

cDNA microarray and data analysis

•

Immunoprecipitation(IP) or Chromatin Immunoprecipitation (ChIP)

•

Immunocytochemistry, Fluorescent and Confocal Microscopy

•

Histopathological analyses

•

shRNA/RNAi manipulation

•

Retroviral & lentiviral mediated mammalian cell transfection system

•

Vector design & gene-cloning and gene-mutagenesis

•

Prokaryotic or eukaryotic recombinant protein expression and

•

purification

Western Blotting/ Southern or Northern Blotting

•

qRT-PCR (Realtime PCR)

•

ELISA/ELISPOT

•

Methylation specific PCR(Ms-PCR), LOH

•

•Animal Models: rats and mice models: e.g. rat surgery; intraperitoneal

injection (IP) or IV injection; In vivo blood collection.

Computer: Microsoft Word, Excel, PowerPoint and Photoshop,

•

GraphPad, FlowJo

RESEARCH EXPERIENCES

Senior Research Associate 2011 --- Present

Duke Human Vaccine Institute, Duke University of School Medicine,

Durham, NC27720

Project1: Immunogenicity of BCG-gp-140( HIV envelope protein)

We generate BCG or BCG mutants carrying HIV gp120 protein.

Mice are immunized with BCG or BCG mutants and boosted with HIV

protein. Splenocytes are isolated, re-stimulated with peptides and analyzed

by FACS to investigate various cytokines secretion by CD4 or CD8 T cells

such as TNFα, IFNγ and IL2; Sera are prepared from those mice and

analyzed by ELISA for antibodies against HIV protein; PBMC are isolated

and analyzed by tetramer staining for specific CD8 or CD4 T cells. Elispot

is performed to detect IFNγ secretion by T cells.

Project 2: Autophagy mediated killing of Mycobacterium tuberculosis by

using LC3 knockout mice model

Senior Research Associate 2006 --- 2011

Department of Pediatrics, Division of Pediatric Allergy and Immunology,

Duke University of School Medicine

Project: Innate and adaptive immunity

• SMAD4 is required for development of maximal endotoxin tolerance in

vitro. We demonstrated that knockdown of SMAD4 by shRNA increased

cytokines secretion and compromised endotoxin tolerance in human THP1

cells by modulation of SHIP1 and IRAK-M.

• Critical role of TSC1 for endotoxin tolerance by inhibiting TLR-induced

innate responses in vivo and in vitro. We showed in vivo and vitro that

knockout of TSC1 in mice increased TNFα, IL6, IL12p40 and iNOS

secretion upon LPS stimulation. TSC1 knockout mice couldn’t develop

endotoxin tolerance.

TSC1 in dendritic cells controls both innate and adaptive immunity. TSC1

•

KO BMDC with the reduced MHCII couldn’t activate T cells compared with

TSC1 wild type BMDC. Overexpressed CIITA rescued MHCII expression.

On the other hand, BMDC with absence of TSC1 increased TNFα and IL-6

secretion after LPS stimulation.

Postdoctoral fellow 2002 --- 2006

Department of Genomics & Genetics, Boston University School of Medicine

Boston, MA02118, USA

Project: Cancer cell genetic and epigenetic changes

• Defining genetic mechanisms that account for individual susceptibility to the

DNA damaging effects of tobacco smoke in lung cancer

• Smad8 role in breast cancer and prostate cancer progression and metastasis

• γ -catenin’s role in cancer progression

Graduate student 1998---2002

Department of Pathology, College of veterinary medicine,

The University of Georgia, GA 30605, USA.

Identification of major factors that regulate Hsp47 and pro-collagen in avian

•

tendon cells.

Generation of the 5’ flank region of chicken TGF-β 4 gene.

•

Expression and characterization of chicken TGF-β 4 proteins in Chinese hamster

•

ovary (CHO) cells and bacteria.

Associate Professor 1997---1998

College of veterinary medicine, Nanjing Agricultural University,

Nanjing, Jiangsu, P R. of China.

• Teaching in veterinary parasitology and pathology.

• Involved in the clinic research projects.

Specialist 1996---1997

International Atomic Energy Agency (IAEA), Vienna, Austria

• Worked on Eradication of tsetse fly by the Sterile Insect Technique (SIT) in

Zanzibar, Tanzanian, sponsored by IAEA/FAO

• Assessment on trypanosomosis incidence and provided advice for tsetse fly

release

• Supervised the technical team and monitored the project progression

Assistant Professor 1990---1994

College of veterinary medicine, Nanjing Agricultural University,

Nanjing, Jiangsu, P R. of China.

Teaching in Veterinary Parasitology.

•

ACADEMIC PUBLICATIONS

Pan Hongjie and Sunhee, Lee. Autophagy Protein LC3 promotes adaptive

1.

immune response against active tuberculosis by inhibiting bacterial burden

and inflammation. In preparation

Pan H, O'Brien TF, Wright G, Yang J, Shin J, Wright KL, Zhong XP.

2.

Critical Role of the Tumor Suppressor Tuberous Sclerosis Complex 1 in

Dendritic Cell Activation of CD4 T Cells by Promoting MHC Class II

Expression via IRF4 and CIITA. Journal of Immunology. 2013 Jul 15;

191(2):699-707.

Pan H, O'Brien TF, Zhang P, Zhong XP. The role of tuberous sclerosis

3.

complex 1 in regulating innate immunity. Journal of Immunology. 2012 Apr

15;188(8):3658-66.

Shin J, Pan H, Zhong XP. Regulation of mast cell survival and function by

4.

tuberous sclerosis complex 1. Blood. 2012 Apr 5;119(14):3306-14.

5. Zhong XP, Shin J, Gorentla BK, O'Brien T, Srivatsan S, Xu L, Chen Y,

Xie D, Pan H. Receptor signaling in immune cell development

and function. Immunol Res. 2011.49(1-3):109-23.

Pan H, Ding E, Hu M, Lagoo AS, Datto MB, Lagoo-Deenadayalan SA.

6.

SMAD4 is required for development of maximal endotoxin tolerance.

Journal of Immunology. 2010. 5502-9.

Papageorgis P, Lambert AW, Ozturk S, Gao F, Pan H, Manne U, Alekseyev

7.

YO, Thiagalingam A, Abdolmaleky HM, Lenburg M, Thiagalingam S. Smad

signaling is required to maintain epigenetic silencing during breast cancer

progression. Cancer Res. 2010 70(3):968-78.

Pan H, Bachelder RE. Autocrine Semaphorin3A stimulates eukaryotic

8.

initiation factor 4E-dependent RhoA translation in breast tumor cells. Exp

Cell Res. 2010 316(17):2825-32.

Pan H, Wanami LS, Dissanayake TR, Bachelder RE. Autocrine

9.

semaphorin3A stimulates alpha2 beta1 integrin expression/function in breast

tumor cells. Breast Cancer Res Treat. 2009 118(1):197-205.

Li Y, Howell EA, Lagoo AS, Kuchibhatla M, Pan H, Cohen HJ, Lagoo SA.

10.

Differential Gene Expression of IRAK-1 and IRAK-M in Peripheral Blood

Mononuclear Cells of Young and Aged Rats Following Preconditioning with

Endotoxin. Shock. 2008 31(1):55-63.

Pan H, Gao F, Papageorgis P, Abdolmaleky HM, Faller DV. Thiagalingam

11.

S. Aberrant Activation of gamma-Catenin Promotes Genomic Instability and

oncogenic Effects during Tumor Progression. Cancer Biol Ther. 2007 6(10):

1638-43.

Abdolmaleky HM, Cheng KH, Faraone SV, Wilcox M, Glatt SJ, Gao F,

12.

Smith CL, Shafa R, Aeali B, Carnevale J, Pan H, Papageorgis P, Ponte JF,

Sivaraman V, Tsuang MT, Thiagalingam S. Hypomethylation of MB-COMT

promoter is a major risk factor for schizophrenia and bipolar disorder. Hum

Mol Genet. 2006 Nov 1; 15(21):3132-45.

Hongjie Pan, Joseph Califano, Jose F. Ponte, Andrea L. Russo, Kuang-hung

13.

Cheng, Arunthathi Thiagalingam, Pratima Nemani, David Sidransky, and

Sam Thiagalingam. LOH Patterns Provide Fingerprints for Genetic

Heterogeneity in Multistep Cancer Progression of Tobacco Smoke-induced

NSCLC. Cancer Res. 2005 65(5):1664-9.

Andrea L. Russo, Arunthathi Thiagalingam, Hongjie Pan, Joseph Califano,

14.

Kuang-hung Cheng, Jose F. Ponte, Dharmaraj Chinnappan, Pratima Nemani,

David Sidransky, and Sam Thiagalingam. Differential DNA

Hypermethylation of Critical Genes Mediate the Stage Specific Tobacco

Smoke Induced Neoplastic Progression of Lung Cancer. Clin Cancer Res.

2005. 11(7)2466-2470.

Halper J., Griffin A, Hu W, Jung C, Zhang J, Pan H, Kisaalita WS, Foutz TL,

15.

Frazier KS In vitro culture decreases the expression of TGF(beta), Hsp47 and

type I procollagen and increases the expression of CTGF in avian tendon

explants. J Musculoskelet Neuronal Interact. 2005 5(1):53-63.

Yoon JH, Brooks RL Jr, Khan A, Pan H, Bryan J, Zhang J, Budsberg SC,

16.

Mueller PO, Halper J. The effect of enrofloxacin on cell proliferation and

proteoglycans in horse tendon cells. Cell Biol Toxicol. 2004 20(1):41-54.

Gang Wang, Cameron Brennan, Martha Rook, Jia Liu Wolfe,

17.

Christopher Leo, Lynda Chin, Hongjie Pan, Wei-Hua Liu, Brendan Price

and G. Mike Makrigiorgos. Balanced-PCR amplification allows unbiased

identification of genomic copy changes in minute cell and tissue samples.

Nucl. Acids Res. 2004, 32-40.

Pan H. and Halper, J. Cloning, expression and characterization of chicken

18.

transforming growth factor beta4. Biochemical and Biophysical Research

Communications (BBRC), 2003, (303) 24-30.

Pan H. and Halper, J. Regulation of heat shock protein 47 and type I

19.

procollagen expression in avian tendon cells. Cell & Tissue Research,

2003,311 (3):373-82.

NATIONAL AND INTERNATIONAL CONFERENCES

Pan Hongjie and Jaroslava Halper. Regulation of heat shock protein 47

1.

expression in avian tenocytes. The Wound Healing Society 10th Annual

Educational Symposium, July-August 2000, Toronto, Canada. (speech)

Hongjie Pan, Joseph Califano, Jose F. Ponte, Andrea L. Russo, Kuang-

2.

hung Cheng, Arunthathi Thiagalingam, Pratima Nemani, David

Sidransky, and Sam Thiagalingam. Gordon Research Conference on

Cancer: Models & Mechanisms’. Salve Regina University, Newport, Rhode

Island, August 1-6, 2004 (poster)

Sam Thiagalingam, H. Pan, J.F. Ponte, K-H. Cheng. The Smad8 connection

3.

to breast cancer. Era of Hope, Department of Defense Breast Cancer

Research Program Meeting June 8-11, 2005. Pennsylvania Convention

Center, Philadelphia, Pennsylvania. (Poster).

Hongjie Pan, Luke S. Wanami, and Robin E. Bachelder,

4.

"Semaphorin3A Increases Expression in Breast Tumor Cells of the

a2b1 Integrin, a Cell Migration Inhibitor:" American Association for

Cancer Research. Advances in Breast Cancer Research: Genetics,

Biology and Clinical Applications. San Diego, CA. Oct.17-20, 2007.

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