ARSEN ZAKARYAN

Telephone: (+*****) *** *** (mobile); (+37460) 501 920 (office); (+37410) 641 481 (home);

E-mails: acazyx@r.postjobfree.com or acazyx@r.postjobfree.com

SCIENCE PROFESSIONAL

Key Knowledge: Molecular Biology, Immunology, Cell Biology, Clinical Diagnostics, Lab

Manager, Project Manager, Veterinary Medicine

Strengths Include: Excellent communication skills, Analytics, Research and Planning,

International working experience, Management experience, fluency in Russian, English and

Armenian

Number of Scientific Publications – 21

EDUCATION

2001 – Doctor of Biology (Ph.D.)

1999-2001 – Ph.D. studentship

1993 – Doctor of Veterinary Medicine (DVM)

1987-1993 – Zoo-Veterinary Institute

PROFESSIONAL EXPERIENCE

CH2MHILL International Services, Inc. USA, Armenian Branch

Cooperative Biological Engagement Program (CBEP) of US Defense Threat

Reduction Agency

THREAT AGENT DETECTION AND RESPONSE (TADR) MANAGER

JAN 2013 – to date

Main Duties:

Leading a subject matter experts group.

Responsible for designing, implementation, initial running (including quality control) and

follow up endurance test of local new medical and veterinary tiered laboratory systems, related

to infectious diseases (including especially dangerous pathogens).

Implementation of quality assurance and WHO Bio-safety and Security requirements into local

medical and veterinary tiered laboratory systems.

Organization of trainings related to BS&S and laboratory diagnostics.

Ensure full and effective communication with close follow-up for project teams in Armenia and

is the US.

~~~

Armenian Branch of Black and Veatch / Southern Research Institute, USA

Cooperative Biological Engagement Program (CBEP) of US Defense Threat

Reduction Agency

HUMAN HEALTH SPECIALIST

JULY 2011 – DEC 2012

Main Duties:

Facilitate development of the CBEP Concept of Operation for biosurveillance in Armenia and

support the development of the Biosurveillance Implementation Plan (BIP). This includes

performing a Gap Analysis of current and desired CBEP capabilities in Armenia for detecting,

diagnosing and reporting of Especially Dangerous Pathogens (EDPs).

Assess current capabilities with respect to molecular diagnostics in the Armenian human health

diagnostic community. Developing plan for incorporating rapid and affordable procedures such

as PCR and ELISA.

Ensure full and effective communication with close follow-up for project teams in Armenia, and

the US.

~~~

“Diagen Plus” Private Medical Diagnostic Laboratory, Yerevan, Armenia

CEO

SEP 2005 – JULY 2011

Main Duties:

Supervision and management of over 20 laboratory employees.

Scientific and healthcare project development and implementation in collaboration with

various international scientific and medical organizations.

Preparation of business plans, organization of co-operation between hospitals and doctors.

Supervision of the internal quality control of laboratory analysis and external quality

assessment scheme (IEQAS), implementation of ISO 9001-2008 into the laboratory work

Preparing and holding seminars and presentations for medical personnel at various medical

organizations.

Selection of laboratory equipment by technical specification, according to laboratory

requirements.

Building relationships with government and commercial structures, conformation and

presentation of financial reports to municipal bodies.

Presenting quarterly financial reports to the board of directors.

~~~

Institute of Molecular Biology, Yerevan, Armenia

Laboratory of Molecular Neurochemistry

GROUP LEADER (part time)

SEP 2005 - JULY 2011

Main duties:

Planning and supervise research experiments.

Analyzing obtained data.

Scientific manuscript preparation and scientific grant applications.

Working with pre- and postdoctoral scientists.

~~~

The University Of Washington, Seattle, USA

POSTDOCTORAL SCIENTIST

JULY 2004 - JULY 2005

Main duties:

Planning and conducting of research experiments.

Analyzing obtained data.

Participation in scientific manuscript writing.

Working with students.

~~~

The Infectious Disease Research Institute, Seattle, USA

POSTDOCTORAL SCIENTIST

AUG 2002 - JUN 2004

Main duties:

Planning and conducting research experiments.

Analyzing obtained data.

Participating in scientific manuscript preparations.

Participating in scientific grant applications.

~~~

Institute of Molecular Biology Yerevan, Armenia

PH.D. STUDENT

JAN 1999 – JULY 2002

Main duties:

Planning and conducting research experiments.

Analyzing obtained data.

Participating in scientific manuscript preparations.

~~~

Scientific Industrial Center "Delta", Yerevan, Armenia

HEAD OF PRODUCTION CONTROL DEPARTMENT

MAR 1998 - JULY 2002

Main duties:

Elaboration, adoption, testing and production of diagnostic test systems.

Quality control.

Production of interim reports.

Negotiation and with various medical and scientific companies.

~~~

Veterinary Station - Murom, Russia

VETERINARIAN

OCT 1993 -NOV 1997

Main duties:

Veterinary control and preventive measures against infectious diseases.

Performing veterinary testing.

Issuing veterinary biosafety certificates for animal husbandry productions.

~~~

OTHER ACTIVITIES OUTSIDE OF ARMENIA

March 2008 – Held a seminar at the University of Konstanz - Konstanz, Germany

February/March 2008 – Guest researcher in a collaborative project at the Universite Pierre et

Marie Curie Paris, France

RESEARCH TECHNIQUES

Animals: Mice handling, BSL-2/3 (agricultural), transgenic mice generation.

DNA: PCR, cloning, transformation, minipreps, maxipreps, SNPs.

Cell Culture: Transient and permanent transfections, growth of transformed cells, T and B

cell hybridomas cell culture.

Proteins: Immunohistochemistry, ELISA, SDS PAGE, bacterial expression, ion-exchange

chromatography, antibody purification, Bradford assay.

Lipids: Isolation, purification and identification via thin-layer chromatography.

CLINICAL LABORATORY TECHNIQUES

Programming, installation and maintenance of laboratory equipment.

Technical support of laboratory equipment.

Clinical chemistry and biochemistry (quantitative determination of substrates, enzymes, and

ions).

ELISA (quantitative determination of hormones, tumor markers, etc).

Microscopic examination including fluorescent.

PCR based methods for testing various infectious diseases and SNPs;

Immunology (quantitative determination of antibody, T and B cells).

MAIN PUBLICATIONS:

1. A. V. Zakaryan, G. S.Kazaryan, G. V. Zakaryan, M. M. Melkonyan, and L. M. Hovsepyan. The Study of Cytokine

Content and Ganglioside Metabolism in Experimental Brain Edema. Biochemistry (Moscow) Supplement Series B:

Biomedical Chemistry, 2011, Vol. 5, No 1, pp. 51-54.

2. Paul C. Engel, Suren Aghajanian, Arsen Zakaryan, Ruzanna Pirumyan and Leon Karabashyan. “Neoscreenpack” – A

Single-Platform Enzyme-Based Technology For Screening Inherited Metabolic Disorders Affecting CNS

Development Of Infants: Pilot Newborn Screening For Phenylketonurea In Armenia. II International Forum Of

Neurologist. Yerevan 2008, Sept 13-14, pp. 188-193.

3. Duthie MS, Kahn M, Zakaryan A, White M, Kahn SJ. Parasite-induced chronic inflammation is not exacerbated by

immunotherapy before or during Trypanosoma cruzi infection. Clin Vaccine Immunol. 2007, 14;1005-1012.

4. Karabashyan L., Zakaryan A., Abramyan R. Phenylketonuria neonatal screening. Methodical recommendation.

Yerevan-2006 (in Russian).

5. Mkhitaryan A., Zakaryan A., Avetisyan D., Karabashyan L. A photometric method for the determination of human

sperm acrosin. The role of substrate in extraction of acrosin from intact acrosome. Medical diagnostics. 2006, 3;104-

109 (in Russian).

6. Hovsepyan L., Djanpoladyan E., Kazaryan S., Zakaryan G., Zakaryan A., Avetisyan I. The influence of cysteine

derivatives on the process of phospholipids peroxidation under condition of brain hypoxia. Biological J. of Armenia.

2006, 58;10-13. (in Russian).

7. Karageuzyan K., Zakaryan A., Hovsepyan L., Zakaryan G., Stepanyan G. Particular properties of lipids composition

in lymphocytes during development. Globe of Science. 2002, 2;44-49.

8. Zakaryan A., Karageuzyan K., Karabashyan L., Hovsepyan L., Stepanyan G. Alterations of glycosphingolipid

composition in bone marrow lymphocyte progenitors, thymocytes and mature lymphocytes in intact and animals with

sarcoma 45. Medical Science of Armenia. 2002, 3;34-38. (in Russian).

9. Mkhitaryan A.G., Zakaryan A., Karabashyan L. Colorimetric test for determination of alkaline phosphatase activity in

blood serum. Medical diagnostics. 2001, 1;134-139. (in Russian).

10. Karageuzyan K., Hovsepyan L., Zakaryan A., Gasparyan G., Sayadyan A., Sarkissyan N. Phospholipid and

ganglioside pattern in chick embryo fibroblast cultures. Proc. of Rus. Acad. Sci. 2001, 377;132-134 (in Russian).

11. Zakaryan A., Karageuzyan K., Hovsepyan L., Karabashyan L., Zakaryan G. Quantitative analysis of phospholipids

and gangliosides in bone marrow progenitors of lymphocytes, thymocytes and mature lymphocytes in tumor-bearing

animals. Int. Immunol. 2001, 13;1141-1145.

12. Karageuzyan K., Hovsepyan L., Zakaryan A., Gambaryan K. Effects of gangliosides on the lipid peroxidation under

conditions of experimental brain oedema. Neurochemistry (Moscow). 2000, 17;211-214 (in Russian).

REFERENCES:

Bill Witting

International Project Manager

Black & Veatch, Washington D.C., USA

E-mail: acazyx@r.postjobfree.com

Prof. Laura Hovsepyan

Head of Department of Molecular Neurochemistry

Institute of Molecular Biology of Armenian NAS

7, Hasratyan St., Yerevan 0014, Armenia

Tel: (37410) 637 607

E-mail: acazyx@r.postjobfree.com

Prof. Leon Karabashyan

Founder of “Diagen Plus” Diagnostic Laboratory

Yerevan, Armenia

Tel: (37491) 213 278

E-mail: acazyx@r.postjobfree.com

Stuart Kahn, MD, PhD

Medical Director, Syntrix Biosystems, Inc. 215 Clay Street NW, Suite B-5

Auburn, WA 98001

Phone: 253-***-**** Ext. 32

E-mail: acazyx@r.postjobfree.com

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