WAEL ABDRABOU

** ****** **., ***. **, Quincy, MA 02169 508-***-**** acaw69@r.postjobfree.com

Profile: http://www.linkedin.com/pub/wael-abdrabou/38/5b5/292/

OBJECTIVE:

Seeking a career in the pharmaceutical/biotechnology field within a highly esteemed institution where I can utilize

my skills and experience

EDUCATION:

Northeastern University, Boston, MA

Masters of Science in Biotechnology April 2013

GPA 3.5/4.0

University of Massachusetts, Boston, MA

Bachelor of Science in Biology July 2007

GPA 3.93/4.0 (Summa cum laude)

SKILLS:

RNA-Seq cDNA Cluster Generation Whole Genome Amplification

Next Generation Sequencing: Designing DNA Primers HPLC

• Illumina Platforms Designing TaqMan Assays Reversed-phase HPLC

DNA & RNA bioanalysis: LC-MS Based Analysis

Sanger Sequencing

• Agilent 2100 Peptide Mapping

RNA Isolation

Protein Chromatography

cDNA library construction Bioanalyzer

Protein Spectrophotometry

• NanoDrop

PCR & qPCR

Protein Staining Techniques

Real Time PCR • Qubit

SDS-PAGE

Reverse Transcription RT-PCR Gel Electrophoresis

DNA Extraction

Software and Data Analysis Tool:

• FastQC

• RNA-SeQC

• Integrative Genomics Viewer

• Vector NTi Sequence Analysis Software

• SPSS Statistical Analysis Software

• Microsoft Office (Word, Excel & PowerPoint)

WORK EXPERIENCE:

Sanofi Pasteur (Discovery Bioinformatics & Genomics) Cambridge, MA

Research Associate (Graduate Co-Op) (July 2013 – Present)

• Assist in implementing Next Generation Sequencing in support of bacterial vaccine Discovery projects

• Develop a pipeline of bacterial infected tissue cultures for differential expression analysis studies

• Develop simultaneous dual RNA-Seq protocol for host tissue cultures and bacterial pathogen.

• Perform cDNA library construction for Next Generation Sequencing (NGS)

• Operate Illumina NGS platforms

• Perform NGS data QC using FastQC, RNA-SeQC, and Integrative Genomics Viewer (IGV)

• Perform cDNA libraries validations and quantifications

• Perform RNA Extractions and RNA integrity validation

• Design species specific Primers

Massachusetts Eye and Ear Infirmary (Ophthalmology Department) Wiggs Laboratory Boston, MA

Research Technologist (July 2007 – May 2013)

• Investigated and screened genes for candidate variants associated with Glaucoma

• Assembled large research populations for Genome Wide Association Studies (GWAS)

• Coordinated participations of research institutions in GWAS Studies

• Generated genotyping data for large populations of Glaucoma patients

• Responsible for DNA concentrations and purities validation

• Performed DNA Sequencing

• Operated DNA and cellular banks

• Processed blood, epithelial and DNA samples

• Participated in diagnostic services quality control assurance processes

Participated in diagnostic services to identify Glaucoma causing mutations ael Abdrabou’s CV - Page 2

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• Trained research fellows and technicians on laboratory techniques

University of Massachusetts, Boston Boston, MA

Research Associate Shiaris Laboratory (Microbiology) (August 2006 – June

2007)

Research Assistant Research Experiences for Undergraduates REU Program (Summer 2006)

• Collected cultures of yeast strains from natural habitats

• Examined and analyzed yeast strains and species

• Performed morphological and microscopical classifications of wild yeast cells and cultures

• Authored a research thesis on yeast strains and species in the wild habitat

• Presented a research thesis at the 13th Annual Massachusetts Statewide Conference

PUBLICATIONS:

• CDKN2B-AS1 Genotype-Glaucoma Feature Correlations in Primary Open-Angle Glaucoma Patients

From the United States. Am J Ophthalmol. 2012 Oct 27.

• The NEIGHBOR Consortium Primary Open-Angle Glaucoma Genome-wide Association Study: Rationale,

Study Design, and Clinical Variables. J Glaucoma. 2012 Jul 23.

• Common Variants at 9p21 and 8q22 Are Associated with Increased Susceptibility to Optic Nerve

Degeneration in Glaucoma. PLoS Genet 8(4): e1002654. doi:10.1371/journal.pgen.1002654

• Common variants near CAV1 and CAV2 are associated with primary open-angle glaucoma in Caucasians

from the USA. Hum Mol Genet. 2011

• Endothelial nitric oxide synthase gene variants and primary open-angle glaucoma: interactions with

hypertension, alcohol intake, and cigarette smoking. Arch Ophthalmol. 2011 Jun

• Reproductive factors and NOS3 variant interactions in primary open-angle glaucoma. Mol Vis. 2011

• Distribution of COL8A2 and COL8A1 gene variants in Caucasian primary open angle glaucoma patients

with thin central corneal thickness. Mol Vis. 2010 Oct 29

• Endothelial nitric oxide synthase gene variants and primary open-angle glaucoma: interactions with sex

and postmenopausal hormone use. Invest Ophthalmol Vis Sci. 2010

PRESENTATIONS:

The Association for Research in Vision and Ophthalmology” “ARVO” Fort Lauderdale, FL

• (2011): The Interaction between Endothelial Nitric Oxide Synthase Gene Variants and Hypertension in

Relation to Primary Open-Angle Glaucoma

• (2011): LTBP2 Sequence Variants in Patients with Congenital Glaucoma

• (2010): The Interaction between Endothelial Nitric Oxide Synthase Gene Variants and Hypertension in

Relation to Primary Open-Angle Glaucoma

• (2009): The Association between Nitric Oxide Synthase 3 Gene Variants and Incident Primary Open-Angle

Glaucoma: A US Population-Based Study

• (2009): Distribution of DNA Sequence Variants in COL8A1 and COL8A2 in Glaucoma Patients with Thin

CCT

The 13th Annual Massachusetts Statewide Conference University of Massachusetts Amherst (2007)

• “Occurrence of the Saccharomyces sensu stricto Complex Members in the Wild Habitat”

UMass Boston Senior Honors Program (2007)

• “Occurrence of the Saccharomyces sensu stricto Complex Members in the Wild Habitat”

UMass Boston – Research Experiences for Undergraduates REU Program (2006)

• “The Ecology of Saccharomyces cerevisiae, “Baker’s Yeast”

AWARDS AND HONORS:

• Senior Honors in Biology

• Departmental Prize of Distinction in Biology

• National Dean’s List (2 semesters)

• Dean’s List (5 semesters)

INTERESTS:

• Soccer, Reading, Chess & Tennis

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