Personal Information

Name Zeyu Xiao Gender Female

E-mail acak6g@r.postjobfree.com & Fax 617-***-****

acak6g@r.postjobfree.com

Cell Phone 617-***-**** Work Phone 617-***-****

**-***, ***** *. **** Institute for Integrative Cancer Research, MIT,

Address

500 Main Street, Cambridge, MA 02139

U.S. Permanent Resident

Visa Status

Education

2008.9 present MIT-Harvard Center of Cancer Nanotechnology Excellence, MIT

David H. Koch Institute for Integrative Cancer Research in MIT; and Harvard Medical

School, Brigham and Women’s Hospital

Postdoc and Research Scientist in Pharmaceutical Sciences

Advisors: Prof. Robert Langer and Prof. Omid Farokhzad

2003.8 2008.7 Chinese Academy of Sciences, Institute of Chemistry, China

Beijing National Laboratory for Molecular Sciences,

Ph.D. in Chemical Biology,

Advisors: Prof. Chunli Bai and Prof. Xiaohong Fang

2006.5 2007.5 University of Florida, Department of Chemistry, Center for Research at the Bio/Nano

Interface

Visiting Graduate Student in Analytical Chemistry,

Advisor: Prof. Weihong Tan

1999.8 2003.7 Peking University, School of Pharmaceutical Sciences, China

B.S. with first class honors in Pharmaceutical Sciences

Industry Experience & Professional Training

2003.1 2004.12 Assistant manager, ReiDeHeTong Pharmacy Co.Ltd., Beijing, China

2002.6 2002.8 Student internship, Pharmacy Department, Beijing Hospital, Beijing, China

2012. 7 Advances in Controlled Release Technology: Polymeric Delivery Systems for

Pharmaceuticals, Proteins, and Other Agents.

Instructor: Robert Langer, MIT professional training program.

2012.6 2012.8 Scientific Writing Course

Instructor: Sonal Jhaveri, Dana Farber Cancer Institute, Harvard Medical School

Research Skills

Related to Drug Formulation

5 years of experiences in the nanoparticle-based drug delivery formulation (e.g., nano-precipitation,

single emulsion, and double emulsion) for the delivery of hydrophilic and hydrophobic

chemotherapeutic drugs, siRNAs, proteins (Postdoc Training);

5 years of experiences in the synthesis of diversified nanoparticles (biodegradable polymeric

nanoparticles, lipid-polymer hybrid nanoparticles, gold nanorods and DNA-assembled

nanoparticles) for drug delivery applications(Postdoc Training);

5 years of experiences in nanoparticle characterization (particle sizing, zeta potential, microscopy,

lyophilization etc.) (Postdoc Training);

1

Related to Chemistry

3 years of experiences in HPLC analysis (Ph.D. and Postdoc Training);

3 years of experiences in TEM, SEM, AFM analysis (Ph.D. and Postdoc Training);

3 years of experiences in UV-VIS spectrometry analysis (Ph.D. Training);

2 years of experiences in polymer synthesis and characterization using NMR (Postdoc Training);

1 year of experience in DNA synthesis (Ph.D. Training).

Related to PK,PD, and in vivo Pharmacology

4 years of knowledge (Bachelor Study) and 6 years of hands-on experiences in pharmaceutical

sciences (Bachelor and Postdoc Training)

3 years of experiences on the tissue distribution and PD study of nanotherapeutics (Postdoc

Training);

1 year of knowledge in the understanding of ADME and PK principles (Bachelor Study);

1 year of experience in the PK study of chemical drugs (Postdoc Training);

2 years of experiences in bioimaging studies in vivo, including bioluminescent imaging and

fluorescence imaging (Postdoc Training);

3 years of experiences in the in vivo therapeutic study, including single agent and

combination therapy (Postdoc Training);

4 years of experiences in the handling of mice and rats, and 1 year of experience in the handling of

rabbits and guinea pigs (Bachelor and Postdoc Training);

3 years of hands-on experiences in the development of tumor mice models (Postdoc Training);

3 years of knowledge of IACUC and writing of animal protocols (Postdoc Training);

1 year of experience in the pathology analysis of tumor tissue (Postdoc Training).

Related to Biological Sciences

10 years of experiences in cell or tissue culture (Ph.D. and Postdoc Training);

10 years of knowledge in cancer biology (Ph.D. and Postdoc Training)

10 years of experiences in confocal imaging (Ph.D. and Postdoc Training)

2 years of experiences in PCR and RT-PCR (Ph.D. and Postdoc Training);

4 years of experiences in flow cytometry analysis (Ph.D. and Postdoc Training);

1 year of experience in ELISA analysis (Ph.D. and Postdoc Training)

1 year of experience in SDS-PAGE analysis (Postdoc Training);

3 years of experience in the design of novel aptamer selection schemes, including Cell-SELEX and

cell-uptake selection (Ph.D. and Postdoc Training);

1 year of experience in the discovery of new cancer biomarkers (Postdoc Training);

4 years of experiences in the mechanism study of membrane receptors involved in endocytosis and

signal transduction using TIRFM (total internal reflection fluorescence microscopy) (Ph.D. Training)

Other Skills

Strong problem-solving skills and high level of initiative in performing experiments, multi-tasking and

troubleshooting from 10 years of research experiences

Excellent scientific writing and oral summarization, publication and presentation skills from publishing 18

high-impact journal papers, and presenting 10 conference talks and 14 invited talks

Excellent organizational, communication and interpersonal skills from 10 years of professional service

and leadership experiences

Works well independently and as a member of a team

Attention to details

Excellent skills in using Word, PowerPoint, Photoshop, Origin, W INNONLIN et al.

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Research Highlight

Research Field: Translational Medicine, Nanomedicine, Nanoparticle-based Drug Delivery, Drug

Formulation, Cancer Diagnostics and Therapy, Cell-Based Aptamer Selection, Targeted Nanoparticles for

Medical Applications, Biomarker Discovery,

Massachusetts Institute of Technology and Harvard Medical School (2008- present)

Postdoctoral Associate

Advisors: Prof. Robert Langer and Prof. Omid Farokhzad

Collaborators: Prof. Jack W. Szostak and Dr. Andrej Luptá Massachusetts General Hospital

ke,

Prof. Steven P. Gygi and Dr. Bo Zhai, Department of Cell Biology, Harvard Medical School

Prof. Bruce R. Zetter, Departments of Cell Biology and Surgery, Boston Children's Hospital

Designed a novel “cell-uptake selection” strategy to enrich cancer-cell specific internalizing RNA

aptamers, which dramatically enhanced the cellular uptake and therapeutic efficacy of the

aptamer-conjugated nanotherapeutics in targeted cancer cells.

This work was published in ACS Nano 2012, and highlighted by C&E News, ACS Nano, Science Daily,

Medical News Today, NanoWerk et al.

Discovered a new internalizing RNA aptamer XEO2mini that can target and enter androgen-independent

prostate cancer cells, conjugated this aptamer with drug-encapsulated polymeric (PLGA-PEG)

nanoparticle (NP), and evaluated pharmacokinetics, bio distribution, and therapeutic efficacy of

NP(docetaxel)-XEO2mini in PC3 prostate tumor-bearing mice models by systemic administration.

The manuscript is in preparation.

Characterized XEO2mini-targeted cancer antigen, which potentially represents a new biomarker for

prostate cancer diagnostics.

The manuscript is in preparation.

Optimized the techniques (e.g., nano-precipitation, single emulsion, and double emulsion) to formulate

PLGA-based polymeric nanoparticles for the controlled release of hydrophobic drugs (docetaxil) and

hydrophilic drugs (doxorubicin), proteins (TGF-ß) and siRNAs.

One product has been licensed to BIND Therapeutics, and currently in Clinical Phase II Trial.

Synthesized and characterized several nanoparticle platforms, including lipid-polymer hybrid

nanoparticles, lipid-polymer-lipid hybrid nanoparticles, surface charge-switching nanoparticles,

subcellular-targeting nanoparticles.

Developed a DNA self-assembled strategy to engineer targeted near infrared-responsive drug delivery

platforms, which provides combined thermo-chemo therapeutic efficacy in multiple tumor-bearing mice

models.

This work was published in Angew Chem Int Ed Engl 2012, and highlighted by Science Daily, Medical

News Today, NanoWerk et al.

University of Florida (2006-2007)

Visiting Graduate Student

Advisor: Prof. Weihong Tan

Collaborators: Prof. Chen Liu and Prof. Ying Li, Department of Pathology, University of Florida

Screened a group of aptamers that specifically bind to liver cancer cells by using Cell-Based SELEX

Discovered and characterized the internalization feature of the sgc8 aptamer, and this is the first reported

internalizing DNA aptamer.

This work was published in Chemistry 2008. This aptamer has been later used by the labmates and other

researches for targeted delivery of drugs or nanoparticles into the cancer cells.

Institute of Chemistry, Chinese Academy of Sciences (2003-2008)

3

Graduate Student

Advisor: Prof. Xiaohong Fang

Collaborators: Prof. Jiachang Yue, Institute of Biophysics, Chinese Academy of Sciences

Prof. Yeguang Chen, Tsinghua University

Set up the platform of single molecule imaging and tracking of membrane receptors in living cells using

TIRFM (total internal reflection fluorescence microscopy)

Explored the activation mechanism of Epidermal Growth Factor Receptor 2/HER2 involved in signal

transduction by using single molecule imaging in living cells

This work was published in J Phys Chem B 2008

Explored the endocytosis pathway of activated EGFR by using single-molecule tracking in living cells.

This work was published in Biochem Biophys Res Commun.2008

Honors/Awards

2013 Harvard Chinese Life Science Distinguished Research Award, Harvard Medical School

2012 Receipt of Green Card under the Category of Aliens with Extraordinary Ability (EB1A)

2011 Scholar-in-Training Awards, American Association for Cancer Research (AACR)

2011 Women in Cancer Research Scholar Awards, AACR

2008 Young Investigator Awards, Chinese Chemical Society, China

Outstanding Student in Academic, Moral and Health Top 2% students in Chinese

2008

Academy of Sciences

2004 2008 Continuous Fellowship in Institute of Chemistry, Chinese Academy of Sciences

Graduate with 1st Class Honors Top 2% students in Peking University

2003

2001 2002 “Ten outstanding student leaders” of Peking University

Continuous 1st Class Scholarship for Excellent Medical Students in Peking

1999 2002

University Top 2% students in Peking University

1999 2001 Continuous Awards of “Outstanding Students in Academic, Moral and Health” Top

2% students in Peking University

2001 2002 Scholarship for Excellent Achievements in Social Activities

Publications (18 in total with 8 as first author; total citations of + 970 times; H-index 13)

1. Xiao Z, Levy-Nissenbaum E, Alex F, Luptak A, Teply B, Chan J, Shi J, Diggar E, Cheng J, Langer R,

Farokhzad OC. Engineering of targeted nanoparticles for cancer therapy using internalizing aptamers

isolated by cell-uptake selection. ACS Nano 2012, 6 (1), 696-704 [Impact Factor: 11.42]

Highlighted in ACS Nano (in Nano 2012, volume 6, issue1) and Chemical &Engineering News

“Getting Cancer Cells to Swallow Poison” in ~30 media, such as Science Daily, Medical News

Today, NanoWerk.

2. Xiao Z, Ji C, Shi J, Pridgen E, Frieder J, Wu J, Farokhzad OC. DNA self -assembly of targeted near

infrared-responsive gold nanoparticles for cancer thermo-chemotherapy. Angew Chem Int Ed Engl

2012, 51(47):118**-***** [Impact Factor: 13.46]

“First-Of-Its-Kind Self-Assembled Nanoparticle for Targeted and Triggered

Thermo-Chemotherapy” in ~ 30 media, including Science Daily, Medical News Today, NanoWerk

3. Xiao Z, Farokhzad OC, Aptamer-functionalized nanoparticles for medical applications: challenges and

opportunities. ACS Nano 2012, 6(5), 3670-3676 [Impact Factor: 11.42]

4. Xiao Z*, Kamaly N*, Valencia PM, Radovic-Moreno AF, Farokhzad OC. Targeted polymeric therapeutic

nanoparticles: design, development and clinical translation. Chem Soc Rev 2012, 41(7), 2971-3010

(*Co-First Authors). Nanomedicine themed issue. [Impact Factor: 28.76] (Invited Review)

5. Xiao Z, Frieder J, Teply B, Langer R, Farokhzad OC Aptamer conjugates: emerging delivery platforms

for targeted cancer therapy. Drug Delivery in Oncology: From Basic Research to Cancer Therapy,

4

Wiley-VCH, 2011, 1690 p., Hardcover, ISBN-13: 978-3-527-32823-9 (Book Chapter)

6. Gao W, Xiao Z, Radovic-Moreno A, Shi J, Langer R, Farokhzad OC. Progress in siRNA Delivery: A

Multifunctional Approach by Polymeric Nanoparticles. Methods in Mol Biol 2010, 629, 53-67

7. Shi J, Xiao Z, Votruba AR, Viols C, Farokhzad OC. Differently Charged Hollow Core/shell

Lipid-polymer-lipid Hybrid Nanoparticles for Small Interfering RNA Delivery. Angew Chem Int Ed Engl

2011, 50, 7027-7031 [Impact Factor: 13.46]

8. Shi J, Xiao Z, Kamaly N, Farokhzad OC. Self-assembled Targeted Nanoparticles: Evolution of

Technologies and Bench to Bedside Translation. Acc Chem Res 2011, 44, 1123-34. Theranostic

nanomedicine special issue. [Impact Factor: 21.64] (Invited Review)

9. Liu Q, Chen B, Wang Q, Shi X, Xiao Z, Lin J, Fang X. Carbon Nanotubes as Molecular Tansporters for

Walled Plant Cells. Nano Lett 2009, 9(3), 1007-1010 [Impact Factor: 13.19]

10. Zhang W, Jiang Y, Wang Q, Ma X, Xiao Z, Zuo W, Fang X, Chen Y. Single Molecule

Imaging Reveals Transforming Growth Factor-β-induced Type II Receptor Dimerization. Proc Natl Acad

Sci U S A 2009,106(37):156**-***** [Impact Factor: 9.68]

11. Xiao Z, Shangguan D, Cao ZC, Fang X, Tan W. Cell-specific Internalization Study of an Aptamer from

Whole Cell Selection. Chemistry 2008, 14, 1769-1775 [Impact Factor: 5.92]

12. Xiao Z, Zhang W, Yang Y, Xu L, Fang X. Single-molecule diffusion study of activated EGFR implicates its

endocytic pathway. Biochem Biophys Res Commun 2008, 369, 730-734 [Impact Factor: 2.48]

13. Xiao Z, Ma X, Jiang Y, Liao J, Yue J, Fang X. Single-Molecule Study of Lateral Mobility of Epidermal

Growth Factor Receptor 2/HER2 on Activation. J Phys Chem B 2008, 112, 4140-4145 [Impact Factor:

3.69]

14. Shangguan D, Meng L, Cao Z, Xiao Z, Fang X, Li Y, Cardona D, Witek RP, Liu C, Tan W. Identification of

Liver Cancer-Specific Aptamers Using Whole Live Cells. Anal Chem 2008, 80(3), 721-728 [Impact

Factor: 5.85]

15. Yang R, Jin J, Chen Y, Shao N, Kang H, Xiao Z, Tang Z, Wu Y, Zhu Z, Tan W. Carbon

Nanotube-Quenched Fluorescent Oligonucleotides: Probes that Fluoresce upon Hybridization.

J.Am.Chem.Soc 2008, 130, 8351–8358 [Impact Factor: 9.90]

16. Shangguan D, Tang Z, Mallikaratchy P, Xiao Z, Tan W. Optimization and Modifications of Aptamers

Selected from Live Cancer Cell Lines. Chembiochem 2007,8,603-606 [Impact Factor: 3.94]

17. Ma X, Wang Q, Jiang Y, Xiao Z, Fang X, Chen Y. Lateral Diffusion of TGF-ß Type I Receptor Studied by

Single-Molecule Imaging. Biochem Biophys Res Commun 2007,356, 67–71 [Impact Factor: 2.48]

18. Xu W, Xiao Z, Zhang T. Mechanical Properties of Silicone Elastomer on Temperature in Biomedical

Application. Materials Letters 2005, 59(17), 2153-2155 [Impact Factor: 2.30]

Conference Presentations (10 in total with 6 as first author)

1. Shi J, Votruba A, Xu X, Xiao Z, Langer R, Zetter B, Farokhzad OC. Hybrid Lipid-Polymer Nanoparticles

for Safe and Effective Delivery of Small Interfering RNA. Poster Presentation, MRS Spring Meeting, April

2012, San Francisco, California, USA

2 Xiao Z, Levy-Nissenbaum E, Aizu W, Langer R, Farokhzad OC. Engineering of targeted nanoparticles for

cancer therapy using internalizing aptamers isolated by cell-uptake selection. Poster Presentation (top

2% highly-rated poster), AACR 102nd Annual Meeting, April 2011, Orlando, Florida, USA

3. Shi J, Xiao Z, Votruba A, Vilos C, Langer R, Farokhzad OC. Differentially charged hollow core/shell

lipid-polymer-lipid hybrid nanoparticle for small interfering RNA delivery. Poster Presentation, Nano in

Cancer: Linking Chemistry, Biology, and Clinical Applications in Vivo, Jan 2011, Miami, Florida, USA

4. Xiao Z, Shi J, Frieder J, Langer R, Farokhzad OC. NIR-responsive drug release system for combined

targeted cancer chemo- and thermo-therapy. Poster Presentation, Nano in Cancer: Linking Chemistry,

Biology, and Clinical Applications in Vivo, Jan 2011, Miami, Florida, USA

5 Xiao Z, Levy-Nissenbaum E, Aizu W, Shi J, Langer R, Farokhzad OC. Engineering of targeted

nanoparticles for cancer therapy using internalizing aptamers isolated by cell-uptake selection. Poster

5

Presentation, Nano in Cancer: Linking Chemistry, Biology, and Clinical Applications in Vivo, Jan 2011,

Miami, Florida, USA

6. Xiao Z, Levy-Nissenbaum E, Aizu W, Chan JM, Gao W, Shi J, Langer R, Farokhzad OC. Uptake

aptamer discovery for prostate cancer therapy. Poster Presentation, 10th US-Japan Symposium on Drug

Delivery Systems, December 2009, Maui, Hawaii, USA.

7. Zhao Z, Xu L, Xiao Z, Shangguan D, Fang X, DNA aptamers selected against Non-Small Cell Lung

cancer. Poster Presentation, 3rd International Symposium on Biomedical Engineering, Bioanalysis and

Nanotechnology, July 2008, Changsha, China (Excellent Poster Prize)

8. Xiao Z, Zhang W, Yang Y, Fang X, Single Molecular Study of Lateral Mobility of ErbB on activation.

Invited oral presentation, 26th Chinese Chemical Society Congress, July 2008, Tianjin, China (Excellent

Presentation Prize)

9. Shangguan D, Xiao Z, Meng L, Li Y, Tan W, High Affinity Aptamers Targeting Biomarkers of Liver

Cancer by Cell-Based SELEX. Poster presentation, Pittcon, April 2007, Chicago, USA

10. Xiao Z, Chen H, Shangguan D, Meng L, Tan W, Aptamers isolated from Cell-Based SELEX for siRNA

delivery. Oral presentation, Pittcon, April 2007, Chicago, USA

Patents

Shi J, Xiao Z, Vilos C, Votruba, A, Langer R, Farokhzad OC. Lipid-polymer hybrid particles.

PCT/US2011/050334.

Invited Talks

I am elected to join the “Visiting-China Delegation” organized by Harvard Medical School, and

2012.12

2013.1 will visit Si Chuan University, Huazhong University of Science and Technology, Shan Dong

University, Peking Union Medical College Hospital, State Food and Drug Administration,

Institute of Zoology, Chinese Academy of Sciences, and Tianjin Medical University

2012.10 Center of Cancer Nanotechnology Excellence (CCNE) annual meeting, MIT

2011.8 Department of Immunology, Harvard Medical School

2009.10 Center of Cancer Nanotechnology Excellence (CCNE) annual meeting, MIT

2008.8 Institute of Botany, Chinese Academy of Sciences, Beijing, China

26th Chinese Chemical Society annual conference, Tianjin, China

2008.7

2006.7 Pharmacology Department, School of Pharmacy, Peking University, China

Professional Service & Leadership

2012 Editor for Bridge Medicine, American Chinese Medical Association

2010 Guest Editor for North American Journal of Medical Sciences (NAJMS)

2010 Journal Reviewer for J. Nanosci. Nanotechnol., Int. J. Polym. Sci., Acta Biomater.,

Biomed. Microdev., and J Biomed Nanotechnol.

2008 Member for American Association for Cancer Research, American Chinese Medical

Association, American Chemistry Society, and Chinese Chemical Society

2010 2012 Vice president, Harvard Medical School Chinese Scholars and Scientists Association

(HMS-CSSA), Boston, USA

2011 2012 Organizing committee, 1st and 2nd Bridge Medical Summit, Boston, MA, USA

2011 2012 Organizing committee, Harvard Life Science Research Symposium 2011 & 2012 and 1st

Harvard America-China Health Summit, Boston, MA, USA

Organizing committee, 40th International Union of Pure and Applied Chemistry

2005

Conference (IUPAC), Beijing, China

Organizing committee, 9th Third World Academy of Sciences (TWAS) General

2003

Conference Beijing, China

6

2003 2006 Student council member, Institute of Chemistry, Chinese Academy of Sciences, China

1999 2003 Student leader, School of Pharmacy, Peking University, China

Supervision Experience (3 graduate, 7 undergraduate & 1 high school students)

Supervisor - Massachusetts Institute of Technology and Harvard Medical School

2012.9 James MacLean, undergraduate student from University of Waterloo, Canada. He designed and

optimized nanoparticle formulations for protein (TGF-ß) delivery, which has been used as a

Present

guideline in the lab and will result in 1 first-authored journal publication.

2012.5 Owen Crookston, undergraduate student from University of Waterloo, Canada. He optimized

double emulsion protocol, which enhanced 4-fold drug encapsulation efficiency. His protocol has

2012. 8

been used by other labmates. He co-authored 1 journal publication for a review paper.

2012.1 Michael Hoang, undergraduate student from University of Waterloo, Canada. He developed

subcellular and cellular-integrated nanoparticles to specifically deliver Dox to its action site

2012.8

(nucleus), which laid the foundation for my future study. He co-authored 1 journal publication for

a review paper.

2011.4 Changwei Ji, Ph.D. student from Nanjing University, China. He optimized the single emulsion

protocol for nanoparticle-based docetaxil delivery for the lab, aided me to evaluate the in vivo

2012.7

therapeutic efficacy of aptamer-targeted nanoparticles by systemic administration, and initiated

the aptamer-medicated cancer biomarker study; which resulted in 2 first-authored and 1

co-authored journal publications.

2010.7 Jillian Frieder, undergraduate student from University of Arizona, USA. She aided me to

examine the in vivo therapeutic efficacy of gold nanoparticles, which resulted in 1 co-authored

2011.4

journal publication and her successful medical school enrollment in University of Iowa Carver

College of Medicine.

2009.9 Tewodros Mamo, undergraduate student from University of Waterloo, Canada. He synthesized

folic acid targeted polymeric nanoparticles. He first-authored 1 review paper, and enrolled the

2010.2

Ph.D. program in University of Toronto.

2009.6 Elise Digga, undergraduate student from Wesley College, USA. She characterized

internalization of aptamers, which resulted in 1 co-authored publication and her successful

2009.10

fellowship application

2009.7 Aanchal Narang, high school student, USA. She learned gold nanoparticle synthesis, and

became interested in the nanomedicine field.

2009.10

Supervisor - Institute of Chemistry, Chinese Academy of Sciences

2007.9 Fang Niu, undergradate student from Lanzhou University, China. Her B.S. thesis under my

guidance was awarded “excellent B.S. thesis” in Lanzhou University.

2008.4

2007.9 Zilong Zhao, 2nd year Ph.D. student in the lab. He learned the Cell-based SELEX technology,

which resulted in 1 first-authored publication.

2008.6

2008.3 Wangxi Luo, 1st year Ph. D. student in the lab. He learned the basic techniques of using TIRFM

to track membrane receptors in living cells, which served as a foundation for his further Ph.D

2008.7

7

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