Ankita Raikar

*** ********** ****, *** *, Boston, MA 02135 acae5h@r.postjobfree.com 857-***-****

CAREER OBJECTIVE:

To pursue a career in a pharmaceutical/biotechnology industry that will give me an opportunity to grow and

demonstrate my scientific skillset

EDUCATION:

Northeastern University, Boston, MA

Masters in Pharmaceutical Sciences (GPA: 3.73) Aug 2013

Related Coursework: Molecular Cell Biology, Anatomy Physiology and Pathophysiology, Concepts in Pharmaceutical

Sciences, Advanced Physical Pharmacy, Experimental Design and Biometrics, Advanced Drug Delivery Systems,

Pharmaceutical Sciences lab course

Mumbai University, Mumbai, India

Bachelor of Pharmacy Jun 2011

Related Coursework: Biochemistry, Microbiology, Biopharmaceutics and Pharmacokinetics, Pharmaceutical Analysis,

Pharmaceutics lab course, Medicinal Chemistry lab course, Pharmacology lab course

EXPERIENCE:

Cancer Biology Intern Jun 2013-Present

Infinity Pharmaceuticals Inc. Cambridge, MA, USA

Isolate and purify Peripheral Blood Mononuclear Cells (PBMCs) from human whole blood

Develop and optimize B-lymphocyte, T-lymphocyte and basophil activation tests on human whole blood for use as clinical

PD assays using multicolor flow cytometry assays (BD LSRFortessa cell analyzer)

Immunophenotyping various surface immunoglobulin (sIg) present on human B-lymphocytes

Perform functional assays on PBMCs to determine IC50s and isotype specificity of several PI3Kinase inhibitors

Develop and optimize ex vivo functional immunoassays on mouse peripheral blood and splenocytes using FACS

Develop surface and intracellular staining panels on mouse peripheral blood, splenocyte and thymocyte samples to detect

effects of PI3Kinase inhibitors on various B- and T-lymphocyte populations using multicolor flow cytometry assays

Perform ELISAs to estimate protein levels in human and mouse plasma samples

Culture and maintain human monocyte U937 cell line and human T-lymphocyte cell lines like MOLT4 and JURKAT

Develop and optimize cell based assays involving protein extraction, quantification and evaluation of Phospho Akt protein

levels using western blots and MSD® MULTI-SPOT Assay System

Analyze data, interpret and document results

Participate in group discussions and present data in small and large company meetings

Pursued Master’s Thesis project “HIF-1α Activation in 3D Tumor Spheroids and Evaluation of 2-Methoxyestradiol

Therapy Using Targeted Polymeric Nanoparticle Formulations.” under the guidance of Dr. Mansoor Amiji

Jan 2012-Aug 2013

Cultured and maintained primary human cell lines like PANC-1 and SKOV3

Developed and optimized in vitro multicellular 3Dimensional tumor spheroids by Hanging Drop Method

Isolated and quantified RNA to compare HIF-1α mRNA levels in spheroids and 2D cell culture using Reverse

Transcriptase-PCR (RT-PCR)

Performed nuclear protein extraction and protein quantification using Micro BCA Protein Assay and Bradford Assay

Used molecular biology techniques like SDS-PAGE, western blots and ELISAs to compare HIF-1α protein levels in

spheroids and monolayer cell culture

Carried out flow cytometry based assays to evaluate surface markers on cells and also to determine HIF-1α inhibition

Performed cell based cytotoxicity assays

Synthesized and purified PLGA-PEG block copolymer and characterized it using 1H-NMR and FTIR analysis

Formulated and characterized non-targeted, Lyp-1 peptide targeted and EGFR peptide targeted PLGA-PEG/PCL

nanoparticles encapsulating 2-Methoxyestradiol

Used analytical techniques like HPLC and UV/Vis Spectroscopy to determine drug encapsulation efficiency of

nanoparticles

Carried out cell permeability studies using confocal microscopy

Interpreted data, analyzed results and presented in lab group meetings

Intern May 2010-Jun 2010

Lupin Ltd, Goa, India

Assisted Quality Control personnel in testing of solid dosage forms for hardness, disintegration, visual properties, friability,

dissolution studies, analyzing drug content in tablets and capsules using HPLC

Submitted detailed report to the training head at end of the training

CONFERENCES ATTENDED:

AAPS Northeast Regional Discussion Group (NERDG) 2013, Connecticut

The 12th Annual Koch Institute Summer Symposium, 2013: Cancer Immunology and Immunotherapy

COMPUTER SKILLS:

MS Word, MS Excel, MS PowerPoint, ImageJ, SPSS, GraphPad Prism, BD FACSDiva Software

EXTRACURRICULAR ACTIVITIES:

Presented poster on ‘Activation of HIF-1α in 3D Ovarian Tumor Spheroids and Evaluation of 2-Methoxyestradiol Therapy

using Polymeric Nanoparticles’ at AAPS NERDG 2013

Presented poster on ‘Nanotechnology in Prevention and Treatment of Cancer’ at Exergy-2009 organized by Institute of

Chemical Technology, Mumbai

Presented poster on ‘Extended Cycle Oral Contraceptives’ at Maharashtra State level Rx festival 2009-2010 organized by

Indian Pharmaceutical Association

Volunteered for ‘No Tobacco Awareness Campaign’ and ‘Safe Use of Medicines Campaign’ organized by Indian

Pharmaceutical Association-Students Forum

REFERENCES:

Available upon request

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