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Quality Control Engineer

Location:
Mattapoisett, MA
Posted:
November 19, 2013

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Resume:

David W Baker

** **** ****** **, ************, MA. 02739

aca3fr@r.postjobfree.com; 508-***-****

Skills Summary:

* ***** ** ******** ********** in areas of Biomaterials, Foreign Body Response, and Tissue Regeneration

3 years of product/ process development experience in medical device formulation development R&D

Laboratory: Proficient in bench-top chemistry, experience with analytical and quality control procedures (SOP),

good laboratory practice (GLP) trained, experience in clean room/ manufacturing/ quality control setting (cGMP

knowledge), proficient with aseptic technique, mammalian cell culture, laboratory management

Instruments: HPLC, FTIR, UV-Vis spectrometer, SEM,TEM, Instron, Rheometer, plate reader, FACS

Cell culture: Tissue digestion, primary cell harvesting, maintenance, passaging, counting, seeding,

cryopreservation & reconstitution, colony isolation, and bioreactor processing of mammalian and tumor cells

Scaffolds: Design of 3D polymer scaffolds and microparticles for cell-incorporation and controlled drug release

Staining: Immunofluorescence, H&E, Masson Trichrome, Picrosirius Red, Oil-Red O, Alcian Blue, Von Kossa

Imaging: Design, testing, and monitoring of in vitro & in vivo imaging probes for cellular interactions

Assays: ELISA, BCA Protein, MTS/MTT, Alamar Blue, Annexin V, Live/Dead

Animal: Murine: care & handling, injections, anesthetization, euthanization, implant /organ excision, surgery.

Porcine: angiogram monitoring of angioplasty, coronary artery extraction, clinical study management

Computer: MS Office, Image J, Photoshop, EndNote, GraphPad, SAS

Excellent organization & interpersonal skills; meticulous, innovative & accurate in research environment;

demonstrated leadership, teaching, and mentoring in personal, group, and team environments

Education:

Ph.D., Biomedical Engineering, University of Texas at Arlington (UTA) Aug, 2013 GPA: 4.0/4.0

Dissertation: The Pivotal Role of Fibrocytes on Foreign Body Reactions

B.S., Chemical Engineering, Mat.Sci. Conc., Clarkson University, Potsdam, NY May, 2005 GPA: 3.2/4.0

Professional Experience:

Graduate Teaching Assistant, Tissue Engineering Laboratory, BE-5365 & BIOL-4365, UTA, (Jun.2010 to present)

Design & develop course syllabus, labs, lectures, and exams (cell culture processes, analysis, 3D scaffold design)

Supervise and teach graduate & undergraduate students, as many as 30 in a class, for a total of 7 semesters

Research Assistant, Regenerative Medicine & Bio-Imaging Lab, UTA, (Jan.2009 to present)

Manage lab and safety regulations, maintain lab inventory, report invoice purchasing transactions

Mentor and supervise other research assistants including; 7 masters students, 5 undergraduates, and 4 interns

Plan, implement, and multitask team/ research projects, in collaboration with multiple departments

Associate Product Development Engineer, Atrium Medical Corp. Hudson NH. (Jan.2007 - Sept.2008)

Development engineer for Drug Coated Stent product and drug formulation research

Process development, troubleshooting, and design: drug/protein formulation & device deployment/ application

Product stability study manager: accelerated aging, material selection, extractables & leachables, sterilization,

packaging, storage, and shipping - leading to selection of product shelf life

Gained experience with porcine clinical trials: setup, operation, instruction, results, and reports

Engineering Technician, Cardiology R&D, Atrium Medical Corp. Hudson NH. (Nov.2005 - Jan.2007)

Gained experience in Design of Experiments with statistical relevance and quality control procedures

Gained experience in data collection, analysis, representation, result summaries, and project reports

Achieved successful outcomes through efficient project management and strategic planning

Worked efficiently in a dynamic team environment in close collaboration with quality control & analytical teams

Research Projects:

Directing fibrocyte adipogenic-differentiation to reduce the fibrotic tissue response

Pioneering investigation of localized differentiation of fibrocytes in vivo at biomaterial interface

Uncovered localized adipogenic differentiation of fibrocytes can reduce collagen production

Baker, 2

Fibroblast/Fibrocyte: surface interaction dictates tissue reaction to micropillar implants

Determined a differential interaction of cell types with micropillar surface cues based on spatial geometry

Revealed an integral role of recruited fibrocytes in response to biomaterial implant topography

Pivotal role of fibrocytes and mast cells in mediating fibrotic responses to biomaterials

Investigated the mast cell relationship to fibrotic responses with stabilization/destabilization compound release

Definitively demonstrated the mast cell /fibrocyte link through c-kit deficient and reconstituted mice

Development of imaging probes for the detection of polarized macrophages

Developed PEG based probes for simultaneous targeting of macrophage subsets during inflammation/ infection

Assessed feasibility of imaging probes in mouse models using in vivo fluorescence imaging system and histology

Nanomaterial cytotoxicity is composition, size, and cell type dependent

Systematically investigated the influence of nanomaterial properties on the degree of cytotoxicity

Established that cell type, nanomaterial size & composition are critical determinants of intracellular responses

Publications (*Co-First Authors)

1. Zhou J, Hao G, Weng H, Tsai YT, Baker D, Sun X, Tang L. In vivo evaluation of medical device-associated inflammation using a

macrophage-specific PET imaging probe. Bioorganic & Medicinal Chemistry Letters (2013) 23:7,2044-2047

2. Baker D, Tang L. Effect of micro-topography on fibrocyte responses and fibrotic tissue reactions at the interface. ACS

Symposium Series (Vol. 1120), Proteins at Interfaces III State of the Art 2012. (2012) 15, 339-353.

3. Cai T, Hu P, Sun M, Zhou J, Tsai YT, Baker D, Tang L. Novel thermogelling dispersions of polymer nanoparticles for controlled

protein release. Nanomedicine: Nanotechnology, Biology, and Medicine (2012) 8:8, 1301-1308

4. Thevenot P*, Baker D*, Weng H, Sun M, Tang L. The pivotal role of fibrocytes and mast cells in mediating fibrotic reactions

to biomaterials. Biomaterials (2011) 32:33, 8394-8403

5. Baker D, Liu X, Weng H, Luo C, Tang L. Fibroblast/Fibrocyte: surface interaction dictates tissue reactions to micropillar

implants. Biomacromolecules (2011) 12, 997-1005

6. Zhou J, Tsai YT, Weng H, Baker D, Tang L. Real time monitoring of biomaterial -mediated inflammatory responses via

macrophage-targeting NIR nanoprobes. Biomaterials (2011) doi:10.1016/j.biomaterials. 2011.08.064

7. Sohaebuddin S, Thevenot P, Baker D, Eaton J, Tang L. Nanomaterial cytotoxicity is composition, size, and cell type

dependent. Particle and Fibre Toxicology (2010) 7:22

8. Nair A*, Baker D*, Tang L. Biological and Chemical Influence on Immune and Regenerative Responses to Biomaterial

nd

Implants. Joint Replacement Technology-2 Edition, Woodhead Publishing (Accepted, Feb. 2013)

9. Tsai YT, Zhou J, Weng H, Tang E, Baker D, Tang L. Optical imaging of fibrin deposition to elucidate participation of mast cells

in foreign body responses. Biomaterials (Accepted, Nov. 2013)

Pending Manuscripts:

10. Baker D, Tsai YT, Weng H, Tang L. Directing Fibrocyte Adipogenic-Differentiation to Reduce the Fibrotic Tissue Response to

Biomaterial Implants. (under review – Biomaterials)

11. Baker D*, Zhou J*, Tsai YT, Patty K, Weng H, Tang E, Nair A, Hu WJ, Tang L. Development of optical probes for in vivo

imaging of polarized macrophages during foreign body reactions. (submitted – Acta Biomaterialia)

Selected Conference Presentations (from a total of 8, including both oral and poster presentations)

Society for Biomaterials 2013 Annual Meeting & Exposition, Boston, MA. Development of imaging probes for the detection

of polarized macrophage subsets during foreign body reactions. (oral presentation: April, 2013)

Society for Biomaterials 2012 Fall Symposium, New Orleans, LA. Macrophage polarization alters fibrocyte interaction at

biomaterial interface. (oral presentation: October, 2012)

27th Annual Southern Biomedical Engineer ing Conference, Arlington, TX. Fibroblast/Fibrocyte: surface interaction dictates

tissue reactions to micropillar implants. (oral presentation: April, 2011)

Awards & Memberships

Graduate Dean’s Doctoral Dissertation Fellowship, UTA, (May 2013 – August 2013)

Student Travel Achievement Recognition (STAR Award). Society for Biomaterials, (April 2013)

Alfred R. and Janet H. Potvin Outstanding Bioengineering Student Award, UTA, (April 2013)

Graduate Assistance in Areas of National Need (GAANN) Fellowship, UTA, (June 2012 – May 2013)

I-Engage Mentoring Program Fellowship, UTA, (May – August 2012)

Science Technology Engineering & Mathematics (STEM) Tuition Fellowship. UTA, (Jun.2010 to present)

Memberships; Tau Beta Pi (President), Phi Kappa Phi (Vice President), Society for Biomaterials, Biomedical Eng Student Soc.



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