Vikas K Goel Ph.D.

abx5b8@r.postjobfree.com

****, **** ***. **

Apt #1

Seattle, WA, USA 98115

617-***-****

HIGHLIGHTS OF QUALIFICATIONS

I am highly motivated, innovative, independent and multi-faceted scientist with research experience

and skills that span both basic science (cell and molecular biology, signal transduction, epigenetic

regulation, and cancer immunology) and translational research (target identification and preclinical

trials). Experience in drug discovery in collaboration with biotech/ pharmaceutical companies

(Halozyme Inc., Merck and Deciphera Pharmaceuticals) and moving therapeutic opportunity

(monoclonal antibodies and small molecule inhibitors) from a cellular concept to proof of principle

confirmation in vivo.

• Managing and leading a team of scientists, proposing and running multiple projects, toward

delivering scientific goals.

• Strong publication record in major journals.

• Productive independent researcher with excellent communication & team skills.

• Adroit at handling problems and amenable to take new challenges.

• Strong organizational skills, exceptional research experience in implementing cutting edge scientific

advances, conception, execution and data analyses of complex scientific studies.

SPECIALITIES

• Comprehensive working knowledge of signal transduction and genetic/epigenetic regulation of

genes. Oncology target identification, validation and analysis of cancer biomarkers.

• Extensive isolation and culturing of islets, Acini and ductal cells from murine pancreas.

• Developed inducible lentivirus siRNA/shRNA to target oncogenes in primary tumor cells and delivery

of viral particles to tumor in murine models.

• Expert in developing and characterization of Genetically Engineered Mouse Models (GEMMs),

orthotopic, syngeneic and xenografts (NOD/SCID and nude) for cancer drug development.

• Small animal Imaging (Ultrasound Vevo 2100 and Xenogen IVIS200), collection and analysis of

tissues and CTC (circulatory tumor cells) of preclinical trials of lead small molecule inhibitors.

• Tissue organotypic, 3D and 2D culture system (carcinoma, fibroblasts and stem cells) in defined

matrices (Collagen, Matrigel) for functional analysis and validation of chemotherapeutics.

• Receptor-ligand interaction studies (CD44, ITAV and OPN) for cellular adhesion and migration.

• Cloning of mammalian, non-mammalian full-length cDNAs (>35) and truncated proteins and

promoters, sequencing, c-DNA library construction, mutagenesis and other DNA and RNA

manipulations.

• Genetic manipulation of primary murine and human cancer cells to produce stable clones.

• Extensive experience in purification and characterization of fibroblasts, immune cells and epithelial

cells from tumors.

• Highly proficient in western blot, in vitro capture assays, Immunoprecipitation, In-situ hybridization

(FISH), Chromatin immunoprecipitation (CHIP), methylation, Acetylation, promoter-reporter assays

(fluorescent and luminescent), and EMSA.

• qPCR (real time PCR), experience in analysis of genomics/transcriptomics (microarray and miRNA)

and proteomics data (SILAC and Mass Spec).

• Collect and prepare DNA, RNA and proteins from various sources such as tumors, blood and other

body fluids of preclinical (murine model) and clinical trials (human).

• Expert in Immunohistochemistry (IHC) on frozen and FFPE tissues, tissue microarrays.

• Proficient in various imaging techniques (bright field, Confocal and multiphoton microscopy) and

quantification/analysis of IHC/IF (multicolor) stains using ImageJ, Aperio Scanscope and Nuance

quantum dot microscope.

• Cellular and molecular assays such as cell proliferation (WST-1, MTT and Brdu labeling),

Apoptosis/cell death (Ki-67, Tunnel and cleaved caspase 3) and cytotoxicity assays.

• Laser Capture Microdissection (LCM) for isolation of RNA/DNA and protein from FFPE tissues.

• Developed in vitro diagnostics PCR assay (IVD) to detect drug resistant Plasmodium from blood.

• Developed ELISA for extracellular matrix components (ECM) for cancer biomarker and diagnostics.

Developed Indirect Immunofluorescence for diagnostics of Rabies virus from clinical samples.

• Expression and purification of various recombinant proteins using bacterial, yeast, viral and

mammalian expression systems (HEK 293, NIH3T3 and Insect cell lines). Protein-protein interaction

studies (antibody antigen, and recombinant protein using Biacore).

• Expert in producing, purification and functional characterization of mouse monoclonal antibodies

(hybridoma technology) and their potential use in therapeutics and diagnostics.

• Synthetic protein production using rabbit reticulocyte expression system.

LICENSING/PATENT

• Genetically engineered mouse model of melanoma (Braf V600E) is licensed to a Biopharmaceuticals

for the development of novel immunotherapy for melanoma.

• Licensing of monoclonal antibodies against Iron Regulated Outer Membrane Proteins of

Acinetobacter baumanii and antibody against nucleoprotein of Rabies virus for development of

diagnostics (by Department of Biotechnology, India).

RESEARCH EXPERIENCE

Mouse model techniques for oncology research: Developed and characterized genetically

engineered mouse models (GEMM), subcutaneous, PDX and orthotopic xenograft (NOD/SCID and

Nude), syngenic and human primary cancer models (tumor bank) for oncology drug discovery and

translational research. I am expert in animal handling techniques such as mouse tumor inoculation

and measurement, oral, sc (subcutaneous) and iv (Intravenous) dosing, blood and tissue collection.

Protein chemistry and antibody production expertise. Studied several different proteins and

peptides employing a wide variety of biophysical and biochemical methods. Expert in expression and

purification of large number of mammalian recombinant proteins from bacteria, yeast, CHO,

HEK293T cells. Studied protein-protein interaction using yeast two hybrids and BIACore resonance

technology. Highly proficient in producing and purification of mouse monoclonal antibodies using

hybridoma technology and their use in diagnostics and therapy. Strong working knowledge of

immunoblot, CO-IP and CHIP on CHIP.

Cell and molecular biology experience: Constructed C-DNA libraries (for Yeast two hybrid

system), cloned several genes in TOPO-TA vector, bacterial, mammalian, reteroviral and lentiviral

expression vectors. Strong working knowledge of FACS analysis purification of primary cells,

invasion, migration, 3D matrix invasion morphogenesis assay, cell proliferation, apoptosis, cell cycle

and luminescence reporter assays, in vitro and in vivo drug assays. Developed several primary

murine and human cancer cell lines.

Computational and Biostatistics

Developed skill sets in analyzing genomics data using Ingenuity Systems Bioinformatics Software

(IPA analysis), protein database, KEGGs pathway, GEO, UCSC Genome browser, Integrative

Genomics Viewer (IGV) and Oncomine. Expert in FlowJo, Soft-Max pro, Adobe Photoshop and

adobe Illustrator. Expert in biostatistics analysis using, GraphPad Prism, Excel and Grafit softwares.

Managerial experience and other responsibilities:

Responsible for design, implementation and management of multiple research projects. Concurrently

advise junior postdoctoral and clinical research fellows. Train and supervise and direct the research

projects of graduate students, technical and research staff. Review and prepare grant proposals,

manuscripts, and presentations at various scientific meetings. Assist in the hiring of technical staff.

EDUCATION

• Ph.D. (Microbial Protein Chemistry) All India Institute of Medical Sciences, New Delhi,

India 2000

• M.Phil. (Molecular Parasitology) University of Delhi New Delhi, India 1995

• M.Sc. (Cell and Molecular Biology) University of Delhi New Delhi, India 1994

• B.Sc. Zoology (Hons.) University of Delhi New Delhi, India 1992

RESEARCH EXPERIENCE

University of Washington Seattle, WA

Research 2013

• Using organotypic and 3D model system studying the role of Immune cells and fibroblasts in

bidirectional cross talk with carcinoma cells in pancreas cancer.

Fred Hutchinson Cancer Research Center Seattle, WA

Research Associate 2009-2013

• Understanding the role of tumor microenvironment (extracellular matrix and fibroblasts) and

TGFβ/Smad signaling in metastasis and drug resistance of pancreatic cancer.

• Preclinical trials using small molecule targeted therapy in mouse model of PDA.

• Analysis of circulating tumor cells (CTC)/cancer stem cell from murine mouse models of cancer.

• Developing a GEMM to study role of oncogenic Kras during pancreas cancer development.

Burnham Institute of Medical Research San Diego, CA

Staff Scientist 2007-2009

• Epigenetic regulation of melanocyte pigmentation and melanoma progression biology by ATF2 and

hypoxia in genetically engineered mouse model system. The work involves extensive signal

transduction, cellular and molecular biology of ATF2.

New England Medical Center Tufts University Boston,MA

Research Associate 2005-2007

• Study the role of BRAF in melanoma biology, which involves the developing and characterization of

BRAFV600E mouse model for melanoma development and progression.

• Preclinical Trials of BRAFV600E specific inhibitors using BRAFV600E mouse model.

Massachusetts General Hospital, Harvard Medical School Boston,MA

Instructor 2004-2005

PostDoctoral Fellow 2002-2004

• Study the role of BRAF in melanoma biology, which involves the developing and characterization of

BRAFV600E mouse model for melanoma development and progression.

• Preclinical Trials of BRAFV600E specific inhibitors using BRAFV600E mouse model.

• Tumor collections from patients for the tumor bank at MGH.

Dept. of Microbiology, All India Institute of Medical Sciences New Delhi, India

Senior Research Associate 1995-2000

• Identification and Characterization of Iron regulated Outer Membrane Proteins of Acinetobacter

baumannii.

Dept. of Zoology, University of Delhi New Delhi, India

Junior Research Associate 1994-1995

• Rapid diagnostics of wild type and drug resistance Plasmodium falciparum directly from patients

blood using PCR.

HONORS and PRIZES

2007 AACR Scholar in training award.

1998 American Society of Microbiology, Scholar in training award.

1998 Indian Association of Medical Microbiologist Dr. S.S. Kelkar Memorial Award.

1995-2000 Council of Scientific and Industrial Research India, Research Scholarship.

1994-1995 University Grant Commission Graduate Aptitude Test Research Scholarship.

PEER-REVIEWED PUBLICATIONS

• Goel VK, Izeradjene K, et.al. Timing of SMAD4 mutation controls a metastatic switch in

pancreas cancer. In preparation.

• Provenzano PP, Cuevas C, Chang AE, Goel VK, Von Hoff DD, etal. Enzymatic targeting of

the stroma ablates physical barriers to treatment of pancreatic ductal adenocarcinoma. Cancer

Cell. 2012 Mar 20;21(3):418-29.

• Meera Shah*, Anindita Bhoumik*, Vikas Goel, et.al. A role for ATF2 in regulating MITF and

melanoma development (PLoS Genetics, December 3;6(12):e1001258.

• Goel VK*, Ibrahim NI, Pemberton TJ, et.al. “Melanocytic nevus-like hyperplasia melanoma

in transgenic BRAFV600E mice”, Oncogene 2009, 28(23): 2289-98.

• Goel VK, Lazar AJ, et.al. Examination of mutations in BRAF, NRAS, and PTEN in primary

cutaneous melanoma. J Invest Dermatol. 2006, 126(1): 154-60.

• Haluska FG, Tsao H, Wu H, Haluska FS, Lazar A, Goel V. Genetic alterations in signaling

pathways in melanoma Clinical Caner Research; 2006,12 (7 Pt 2):2301s-2307s

• Tsao H*, Goel V*, Wu H, Yang G, Haluska FG. Genetic interaction between NRAS and

BRAF mutations and PTEN/MMAC1 inactivation in melanoma. J Invest Dermatol. 2004,

122(2):337-41 *Equal first author Contribution.

• Wu H, Goel VK, Haluska FG. PTEN in melanoma cancer. Oncogene.2003, 22(20): 3113-

22.

• Goel VK, Li Xuerong, Liu S C, Chisthi AH and Oh SS. Sialic Acid independent invasion of

Plasmodium falciparum in red blood cell. Proc. Natl Acad Sci (USA). 2003, 100(9): 5164-9.

• Hanspal M, Goel VK, Raphel P, Oh SS.Chisthi A. Erythrocyte Calpain is dispensable for

malaria parasite invasion and growth. Molecular and Biochemical Parasitology. 2002,

122(2):227-229.

• Goel VK, Arti Kapil. Monoclonal antibodies to immunogenic Iron Regulated Outer

Membrane Proteins of Acinetobacter baumannii are bactericidal. BMC Microbiology, 2001; 1: 16.

• Goel VK, Arti Kapil, et.al. Effect of iron on growth and extra cellular products of

Acinetobacter baumannii. Japanese J. of Medical Sciences and Biol. 1998, 51(1): 25-33.

• Anita Pandey, Arti Kapil, Seema Sood, Goel VK, et. al. In vitro Susceptibility of

Ampicillin/Sulbactum and Amoxicillin/Clavulanic acid against Acinetobacter baumannii. J. Clinical

Microbiology.1998, 36(11): 3415-3416.

• Arti Kapil, Sumeeta Gulati, Goel VK, Lalit Kumar, et. al. Investigation into an outbreak due

to Acinetobacter baumannii in a high risk ward. Medical Oncology 1998, 15(4): 270-274.

• Seema Sood, Goel VK et.al. Paratyphoid fever in India: an emerging problem!

Emerging and Infectious Diseases, 1999, 5(3): 483-484.

ABSTRACTS (Selected)

• Goel VK et.al. Modulation of pancreas cancer invasion and metastasis by SMAD4/DPC4

signaling. Presented at 102nd Annual meeting of American Association of Cancer Research, 2011.

• Goel VK, Helen H Wu, et.al. Mice transgenic for BRAF V600E demonstrate phenotype

affecting melanocyte and neural lineages. Presented at 97th Annual meeting of American

Association of Cancer Research, 2007.

• Goel VK, Li Xuerong,Liu et.al. Band 3 dependent invasion of red blood cells in Plasmodium

Falciparum. Presented in Annual meeting of American Society of Tropical medicine and Hygiene at Atlanta,

November 2001.

• Goel VK and SS Oh, Liu D, Chisthi AH. Band 3 knock out mice are completely resistant to

Plasmodium infection. Presented at Molecular Parisitology Meeting (2001) at Woodshole, Boston, USA.

• Goel VK and Arti Kapil. Iron Regulated Outer Membrane Proteins of Acinetobacter baumannii are

virulence determinants. Presented in 38th International congress of antimicrobial agents and chemotherapy

Held at San Diego USA in September 1998.

• Goel VK and Arti Kapil. Opsonophagocytic activity of monoclonal antibodies against IROMPs of

Acinetobacter baumannii. Presented in 10th International Congress of Immunology held at New Delhi in

November 1998.

• Goel VK, Arti Kapil, et.al. Monoclonal Antibodies to the Iron Regulated Outer membrane Proteins of

Acinetobacter baumannii are bactericidal. Presented in the 8 th International Congress of Infectious Diseases

held at Boston USA in May 1998.

MEMBER- PROFESSIONAL SOCIETIES

American Association of Cancer Research

REFRENCES

Dr. Phil Hinds Ph.D. Dr. Ze’ev Ronai

Professor of Radiation Oncology, Professor and Associate Director

Tufts University School of Medicine SanFord Burnham Medical Research Inst.

Phone: 617-***-**** 858-***-****

email: abx5b8@r.postjobfree.com abx5b8@r.postjobfree.com

Dr. Venu G. Pillarisetty, M.D. Dr. Paul Lampe

Assistant Professor Professor

Department of Surgery Public Health Sciences

University of Washington Fred Hutchinson Cancer Research Center

Office: 206- 6164924 Office: 206- 667-4123

email: abx5b8@r.postjobfree.com email: abx5b8@r.postjobfree.com

Dr. Frank G. Haluska, M.D., Ph.D

Vice President, Clinical Affairs, ARIAD Pharmaceuticals

26 Landsdowne Street, Cambridge, MA 02139

Phone:617- 494 0400 ext 243, email: abx5b8@r.postjobfree.com

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