Kimberly Ann Hoyt, M.S.

619-***-****

absybu@r.postjobfree.com

San Diego, Ca 92109

Profile

Biologist with experience in the practical application of biochemistry and molecular biology techniques is seeking Research Associate position.

• Extensive experience with MS office, spreadsheets, data entry, data analysis, computation, analytical processes, statistical analysis, data graphics program, and problem solving.

• Background in biochemistry, molecular biology, and protein laboratory techniques.

• Ability to work interactively with multi functional disciplinary teams as well as independently.

• Detailed oriented and good record keeping skills.

• Adherence to SOPs/universal precaution guidelines and GMP practices.

• Working knowledge of laboratory methods, clean laboratory skills, preparations of reagents, buffer solutions, laboratory safety, hazardous material procedure, and regulatory practices.

• Experience in flow cytometry, organizing, assembling, and implementing comprehensive laboratory equipment.

• Successful training of undergraduate science majors in biochemistry laboratory techniques.

Methodology and Skill set

Molecular – FacScan Flow Cytometry, Apoptotic DNA analysis techniques, Bacteria plasmid vector, PCR, agarose electrophoresis, bacteria identification.

Biochemistry – Bioassays, cell culture, growth media preparation, protein purification, spectrometer, SDS-Page, autoclaving.

Other – Trace metal analysis, protocols and method development for trace metals analysis in various samples (ICP-OES, ICP-MS), mercury/monomethylmercury analysis following US-EPA method (CV-AFS).

Experience

Environmental Consultant Environmental Inspector / Biological monitor March 2011 – Present

Chambers Group Inc. 9909 Huennekens St., San Diego

• Conducting biological surveys, habitat assessments, and focused surveys for federal- and state-listed species and California species of special concern.

• Working knowledge of southern California biota, and have conducted focused surveys for sensitive plant species in San Diego County.

• Have performed environmental reviews, monitoring for biological resources, biological and executive summary reports

Biotechnology Firm Lab Support – On Assignment January 2011 – March 2011

Sayrna Technologies 6990 Carroll Rd., Ste A

• Operation of Flow Cytometer measuring enzyme activity and inhibition with various chemical assays.

• GMP Practices.

• Tecan FreedomEvo robotic platform workstation.

Biotechnology Firm Laboratory Technician February 2010 – November 2010

Phoenix Flow Systems 6790 Top Gun St #1, San Diego

• Development of bioassays protocol used for testing of enzymatic activity.

• Assembly of TUNEL assays kits and reagents kits.

• Implemented bioassays for measuring the stability of control cells.

• Operation of Flow Cytometer FacScan, in the measurement of cells in a flow system.

• Adhere to SOPs/universal precaution guidelines.

• Quality control by in process and final product testing.

• Created reports/summaries/protocols. Generate appropriate audit trail for all activities.

• Microbiology lab assistant/technician. Maintain laboratory equipment/inventory levels.

• Prepare or preserve buffers/reagents.

• Assembly and implementation of comprehensive manufacturing and production equipment.

• Ability to perform cleaning and sterilization of manufacturing/production equipment.

Graduate Student (laboratory of Michael Latz) 2007- 2009

University of California, San Diego

• Discovered that particle size interactions with substances is one of the main factors influencing bioavailability to plant tissue.

• Investigated particle characteristics to size and organic content, dissolved ion substances forms, kinetic rate of tissue uptake, and affect on various plant tissues in bioavailability of substances.

• Established two fold experimental design to determine environmental factors affecting contaminant accumulation and kinetic rate of uptake in plant tissues.

• Implemented induced coupled plasma mass spectrometer and cold vapor fluorescence mass spectrometer for measuring substance content levels in tissue and sediment samples.

• Developed protocol for nitric acid digestion of plant tissues and sediment for the analysis of substances.

• Obtained funding through Grant writing for research

• Demonstrated organizational, communication, data analysis, presentation, and report writing.

Teaching Assistant

University of California, San Diego $1,800.00 month- part time work (20 hr wk)

Biochemistry Lab Techniques March-June 2009

Cell Biology March-June 2008

Structural Biochemistry September-December 2007

Undergraduate (laboratory of Doug Bartlett) January 2006 - February 2007

University of California, San Diego

• Implemented molecular biology techniques.

• Determined bacterial content of Venice Lagoon samples.

REU Research Experience for Undergraduate (Roland Lamberson’s Lab) June - August 2005

Humboldt State University

Education

Quality Assurance and Control Certificate Program January 2010 – Current (online courses)

UCSD Extensions Life Sciences

M.S. Biology Graduated September 2009

University of California, San Diego GPA – 3.91

Advisor: Deheyn D. Dimitri

Thesis title: Levels of Metals from Salt Marsh Plants from Southern California, USA

B.S. Biochemistry and Cell Biology Graduated June 2007

University of California, San Diego GPA – 3.65

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