RESUME November, ****

YUVAL SHOHAM

I.D. ********

Born: 8/16/1953, Kibbutz Einat, Israel

Marital Status: Married + 2

Private Address: 34 Vitkin St., Haifa 34755, Israel

Phone: 972-4- 8244706, 052-*-******

Business Address: Dept. of Biotechnology & Food Engineering, Technion,

Technion City, Haifa 32000 Israel

Phones: 972-*-*******, (office), 972-*-***-****, -2928/7 (lab)

Fax: 972-*-*******

E-mail: abql7k@r.postjobfree.com

ACADEMIC DEGREES

1977 - 1980 B.Sc (with honors), Tel Aviv University, Israel, Biology.

1981 - 1982 M.Sc (with honors), Tel Aviv University, Israel, Microbiology.

1982 - 1987 Ph.D., M.I.T, Cambridge, MA, USA, Biochemical Engineering.

ACADEMIC APPOINTMENTS

1983 - 1987 Research Assistant, Massachusetts Institute of Technology, Cambridge,

1988 - 1991 Lecturer, Dept. of Biotechnology and Food Engineering, Technion

1992 - 1996 Senior Lecturer

1997 - 2001 Associate Professor

2001 - 2008 Head, The Interdepartmental Program in Biotechnology, Technion

2002 - Professor

2002 - The Erwin and Rosl Pollak Chair in Biotechnology

2002 - 2003 Deputy Dean, Faculty of Food Engineering & Biotechnology

2004 - 2007 Dean, Faculty of Biotechnology and Food Engineering

2004 - 2007 Director, The Otto Meyerhoff Minerva Center for Biotechnology

2008 - 2009 Deputy Dean, Faculty of Biotechnology and Food Engineering

2009 - Deputy Executive Vice President for Research

2010 - Director, The Lorry I. Lokey Interdisciplinary Center for Life Sciences and

Engineering

RESEARCH INTERESTS

*Structure-function and protein-engineering of glycoside hydrolases,

aminopeptidases and lipases

*Molecular characterization of the cellulosome complex from

Clostridium thermocellum

*Gene regulation of hemicellulolytic and cellulolytic genes in Geobacillus

stearothermophilus and Clostridium thermocellum

*Biotechnology of thermophiles and thermostable enzymes

*Biodegradation of lignocellulose and hemicellulose material for

applications in the paper industry

*Biotransformations for the production of natural flavors and fragrances

TEACHING EXPERIENCE

1981 - 1982: Teaching Assistant, Tel Aviv University, Advanced Microbiology.

1986 - 1987: Teaching Assistant, MIT, Laboratory in Applied Biology.

1988 - Dept. of Food Engineering and Biotechnology, Technion.

Undergraduate (U) and Graduate (G) courses:

1) Molecular Biotechnology I (U)

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2) Process Biotechnology II (G/U)

3) Biotechnology Laboratory,(U)

4) Advanced Laboratory in Biochemical Engineering

5) Downstream Processes in Biotechnology (U,G)

TECHNION ACTIVITIES

1994 - 2001 INNmember

1996- Member of the Technion Biotechnology Committee

1996-2001 Member of the Technion Computer Committee

1997- Scientific Director, Minerva Center for Biotechnology

2001-2008 Head, The Interdepartmental Program in Biotechnology

2002-2004 Technion s Patent Committee

2003-2004 Technion s Committee for Promotion and Tenure

2008 - Technion s Professional Committee for Promotion and Tenure

MEMBERSHIP IN PROFESSIONAL SOCIETIES

The American Society for Microbiology (Fellow of the Academy, 2001 -)

The American Chemical Society

Israel Society for Microbiology Board member 2001-2005, President 2009-

Israel Institute of Chemical Engineering

The Israel Chemical Society

American Association for the Advancement of Science

The Protein Society

Society for General Microbiology

HONORS

1980 B.Sc (with honors), Tel Aviv University

1982 M.Sc (with honors), Tel Aviv University

1990 The Muriel and David Jacknow Award for excellence in teaching.

1995 The Henry Taub Prize for Research

1996 The Herschel Rich Technion Innovation Award

1999 The Moshe Shilo Award from the Israel Society for Microbiology

2001 Fellow-American Academy of Microbiology

2002 The Eduard Rherin Science Prize of the German Technion Society

2002 The Erwin and Rosl Pollak Chair in Biotechnology

2007 The Technion s Excellence in Teaching Award (top 5%).

2009 The Herschel and Hilda Rich Technion Innovation Award

PARTICIPATION IN ORGANIZING CONFERENCES

1) Bat-Seva de Rothschild Foundation symposium on "Use of Natural and Genetically

Microorganisms to Combat Pollution Kiryat Anavim, Israel, May 1992. Member of

the Scientific Committee.

2) Environmental Biotechnology Workshop. Haifa, November 1995. Organizer and

Chair.

3) Israel Institute of Chemical Engineering Annual Meeting. Tel Aviv, 1996. Session

Chair: Biotechnology

4) Advances in Biotechnology Workshop, Haifa, February, 1997. Session Chair.

5) 14th Umbrella-Symposium on Biotechnology. Juilich, Germany November 1997.

Head of Technion's Delegation and Session Chair.

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6) 8th international Symposium on the Genetics of Industrial Microorganisms

(GIM), Jerusalem, Israel, June 1998. Local Organizing Committee.

7) 15th Umbrella-Symposium on Biotechnology. Haifa, November 1998, Scientific and

Organizing Committee.

8) International Symposium on Polysaccharide Biotechnology: An Interdisciplinary

Approach. Ber Sheva, May 1998. Scientific Program Committee.

9) Israel Society for Microbiology, Annual Meeting. Rehovot February, 1998. Session

Chair: Applied Microbiology.

10) The Tri-National American-Turkish-Israeli Conference in Chemical Engineering.

Haifa, March 1999. Session Chair: Biotechnology.

11) Gordon Research Conference on Cellulases and Cellulosomes. Andover, New

Hampshire, July 1999. Discussion leader.

12) Israel Society for Microbiology, Annual Meeting. Haifa 2000. Head, Organizing

Committee.

13) Enzymillennium: from Enzyme Evolution to Industrial Biocatalysis-A Binational UK-

Israeli Symposium on Biotransformation and Biosynthesis. Tel Aviv, February 2000.

Organizing Committee.

14) Israel Society for Microbiology, Annual Meeting. Jerusalem February, 2002. Session

Chair: Food Microbiology and Molecular Biotechnology.

The 68th Meeting of the Israel Chemical Society, Tel Aviv, January, 2003. Session

15)

Chair: Structure and Function of Biomacromolecules

16) Israel Society for Microbiology, Annual Meeting. Tel Aviv, February, 2003 Session

Chair: Applied and Environmental Microbiology

17) Israel Society for Microbiology, Annual Meeting. Haifa, February 2004. Head,

Organizing Committee

18) Israel Society for Microbiology, Annual Meeting. Tel Aviv, February, 2005 Session

Chair: Applied and Environmental Microbiology

19) Half a Cemtury of the Carbohydrate-Protein interface: An International Symposium to

Celberate the 80th Birthday of Prof. Natan Sharon. Rehovot, Nov 23-24, 2005.

Session Chair: Glycotechnology

20) From biomass to biofuels, a roadmap to the energy future, Workshop, Rockville, MD

December 7-9, 2005.

21) Gordon Research Conference on Cellulases & Cellulosomes, Discussion Leader,

Prokaryote Genomics, Procter Academy, Andover NH, July 29 August 3, 2007.

22) Nano Engineering Meets Life Sciences Symposium, Session Chair, Hannover

Convention Center, Hannover, Germany, October 8-10, 2007.

23) The International Conference of the Israeli Food Industires, Session Chair, Lod

Avenue Center, June 23-24, 2008.

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GRADUATE STUDENTS

Ph.D

1. Orit Gat, D.Sc. 1996 Cloning, DNA sequence, and characterization of the

xylanase gene from Bacillus stearothermophilus T-6.

2. Ofer Shenker, D.Sc. 1997. Regulation of xylanase production by Bacillus

stearothermophilus T-6.

3. Eyal Shimoni, D.Sc. 1998. Bioconversion of essential oils to natural flavors and

fragrances.

4. Sarah Gilead-Gropper, Ph.D. 1998. Cloning sequencing and regulation studies of L-

arabinose utilizing genes from Bacillus stearothermophilus T-6.

5. Adva Mechaly (Zmora), Ph.D. 1998. Structure function studies on xylanase T-6.

6. Sima Yaron, Ph.D. 1999. Structure and interactions of functional domains from the

cellulosome of Clostridium thermocellum.

7. Smadar Shulami, D.Sc. 2000. Molecular characterization of the glucuronic acid

utilization operon from Bacillus stearothermophilus T-6.

8. Galit Meshulam, Ph.D. 2001. Isolation and characterization of lipases from

thermophilic bacteria for the preparation of optically active compounds.

9. Tal Dror, D.Sc. 2003. Regulation of the cellulsome complex of Clostridium

thermocellum.

10. Tsafrir Bravman, Ph.D. 2003. Mechanistic studies on Family 39 & 52 xylosidases.

11. Galia Zaide, Ph.D. 2004. The regulation of the xylanolytic system from Geobacillus

stearothermophilus T-6.

12. Gennady Zolotnitsky. Ph.D. 2004. Characterization of enzyme substrate interactions

in xylanase T-6.

13. Dalia Shallom, Ph.D. 2005. Structure-function studies on -glucuronidase and -

arabinfuranosidase from Geobacillus stearothermophilus.

14. Tal Hendelsman. Ph.D. 2005. Protein-protein interactions in the cellulosome

complex of Clostridium thermocellum.

15. Ifat Fondiano, Ph.D. 2007. Biochemical characterization of bacterial aminopeptidases.

16. Einat Naveh Abramovich. Ph.D. 2008. Structure-function studies on arabinanases

from Geobacillus stearothermophilus.

17. Alon Ben-David, Ph.D. 2009. Engineering glycoside hydrolases for novel applications

and properties.

18. Yakir Nataf, Ph.D. 2010. Genome-wide gene expression analysis of the cellulose

degrading systenm in Clostridium thermocellum.

19. Dan Goldman, Ph.D. 2011. Biochemical characterization of Zymomonas mobilis

levansucrase.

20. Orly Tabachnikov, Ph.D. 2012. Biochemical characterization of the arabinan

degrading enzymes in Geobacillus stearothermophilus.

21. Onit Sikari, Ph.D., 2013. The catalytic mechanism of xylan-acetyl esterases from

Geobacillus stearothermophilus T-6

M.Sc.

1. Anat Carmi, M.Sc.1990. Isolation of lignin decomposing bacteria.

2. Alex Khasin, M.Sc. 1991. Purification and characterization of a xylan-degrading

enzyme from Bacillus stearothermophilus T-6.

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3. Ronen Neutra, M.Sc. 1992. Optimization of the production of recombinant

protein using baculovirus expression system in insect cell culture. (Additional

supervisor: Dr. Ben Zion Levi).

4. Zeev Schwartz, M.Sc. 1992. Production of xylanases from thermophiles.

5. Cecilia Regueros, M.Sc.1992. Cloning and expression of the Bacillus

stearothermophilus T-6 xylosidase gene.

6. Tal Hendelsman, M.Sc. 1992. The isolation and characterization of lipases from

thermophiles.

7. Ayelet Melnik, M.Sc. 1993. Continuous production and purification of xylanase T-6.

(Additional supervisor: Prof. Z. Berk).

8. Tsvia Erlich, M.Sc. 1993. The thermoinactivation mechanisms of xylanase T-6.

9. Eilat Ezra, M.Sc. 1993. Extraction and purification of lycopene from Phycomyces

blakesleeanus. (Additional supervisor: Prof. Z. Berk).

10. Sarah Gilead, M.Sc. 1993. Purification and characterization of an -L-

arabinofuranosidase from Bacillus stearothermophilus T-6.

11. Adva Zmora, M.Sc. 1995. Overexpression of the Bacillus stearothermophilus T-6

xylanase.

12. Smadar Herzog, M.Sc. 1995. DNA sequencing and characterization of the -

xylosidase operon from Bacillus stearothermophilus T-6.

13. Galia Yarma, M.Sc. 1997. Protein production in a hollow fiber reactor (Primary

surervisor: Prof. M. Sheintuch).

14. Iris Raz, M.Sc. 1999. Methyltransferases involved in the formation of methyl-chavicol

and methyl eugenol in sweet basil (Additional supervisor: Dr. E. Lewinsohn).

15. Galia Zaide, M.Sc. 1999. Biochemical characterization of -glucuronidase from

Bacillus stearothermophilus T-6.

16. Gennady Zolotnitsky, M.Sc. 1999. Cloning and characterization of the xylan-

utilization genes from Bacillus stearothermophilus T-6.

17. Gilad Rachel, M.Sc. 2000. Characterization of the non cellulosomal cellulase CelI

from Clostridium thermocellum.

18. Mirit Kolog, M.Sc. 2000. Overexpression and characterization of the intracellular

xylanase from Bacillus strearothermophilus T-6.

19. Yael Chertkow, M.Sc. 2000. Heterologous expression of cellulases from Clostridium

thermocellum.

20. Ayelet Baumzweig, M.Sc. 2001. Probing surface residues for enhanced

thermostability in xylanase T-6.

21. Adi Rolider, M.Sc. 2002. Fermentation studies on the production of the cellulosome by

Clostridium thermocellum.

22. Larisa Rabinovitch, M.Sc. 2002. Cloning, overproduction and biochemical

characterization of SGAP, an amino peptidases from and Streptomyces griseus.

23. Orit Gershon, M.Sc. 2002, Cloning, overexpression, and biochemical characterization

of KDO8PS from Aquifex pyrophilus. (Primary supervisor Timor Baasov).

24. Ifat Fondiano, M.Sc. 2003. Overproduction and biochemical characterization of the

ywaD gene product, an aminopeptidases from Bacillus subtilis.

25. Shira Raikin, M.Sc. 2005. Isolation and charcterization of lipases with unique

specificities.

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26. Elinor Malul, M.Sc. 2005. Site-specific labeling of proteins via an in-vitro translation

system.

27. Yulia Lipman, M.Sc. 2006. Structural elements in lipase T-6 that affect hydrolysis of

triglycerides at the sn-2 position.

28. Yael Langut, M.Sc. 2006. Characterization of the repressor XylR from Geobacillus

stearotermophilus.

29. Yael Grimpel, M.Sc. 2006. Cloning, expression and biochemical characterization of

acetyl esterases from Geobacillus stearothermophilus T-6.

30. Ram Nechooshtan, M.Sc. 2006. Characterization of regulatory elements of the

cellulase system in Clostridium thermocellum.

31. Maya Leon, M.Sc., 2006. Biochemical characterization of a XynB3, a beta- xylosidase

from Gebacillus stearthermophilus.

32. Margarita Volkinshtein, M.Sc. 2008. Biochemical characterization of acetyl esterases

from Geobacillus stearothermophilus T-6.

34. Eran Ivanir, M.Sc., 2010. Site-specific labeling of proteins.

35. Itzhak Shner, M.Sc. 2010. Identifying regulatory elements in Geobacillus

stearothermophilus.

36. Arik Zehavi, M.Sc. 2011. Developing genetic tools for G. stearothermophilus.

POST DOCTORATE FELLOWS

1. Aviva Lapidot, Ph.D (Levi Eshkol Post Doc. Scholarship) 1992-1994. Heterologous

Expression of the xylanase gene in B. subtilis and E. coli.

2. Orit Gat, Ph.D 1996-1997. Heterologous expression of the cellulosome complex from

Clostridium thermocellum.

3. Sarah Gilead-Gropper, Ph.D. 1999-2000. Structure-function relationship of KDO8P.

4. Smadar Shulami, Ph.D. 2000-2003. Structure-function relationship of KDO8P:

Rational design of novel antibacterial drugs.

5. Noa Lavid, Ph.D. 2001-2004. Isolation of novel lipases for organic synthesis.

6. Tali Dror. Ph.D. 2002-2003. The regulation of the cellulosome complex in

Clostridium thermocellum

7. Tsafrir Bravman, Ph.D., 2004. Organic synthesis with glycosynthases

8. Gennady Zolotnitsky, Ph.D., 2004-2005. The binding thermodynamic of CBD to

cellulose

RESEARCH ASSISTANT FELLOW

Iris Alchanati, M.Sc. 1989-1995.

Smadar Shulami, Ph.D, 2004-

Noa Lavid, Ph.D., 2005 -

RESEARCH GRANTS

1. BIOVICK AB & CO KB, and KORSNAS, Sweden. "Biobleaching of paper pulp"

1988 - 1993: $ 1,100,000. Y. Shoham and E. Rosenberg

2. G.B.F "Baculovirus expression system" 1990 - 1992: DM 100,000. Y. Shoham and

B.Z. Levi.

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3. UNITED STATES-ISRAEL BINATIONAL SCIENCE FOUNDATION (BSF).

"Regulation of hemicellulytic operons in Bacillus stearothermophilus"

1994-1996: $ 110,000. Y. Shoham and A. L. Sonenshein.

4. ISRAEL MINISTRY OF SCIENCE. "Enhancment of thermal and pH stability of

hemicellulases via functional studies structural analysis and rational protein

engineering" 1994-1996: NS 110,000. Y. Shoham and Gil Shoham.

5. MINERVA FOUNDATION. "The regulation of the xylanase and -L-

arabinofuranosidase genes in Bacillus stearothermophilus" 1995: DM 25,000. Y.

Shoham

6. THE BASIC RESEARCH FOUNDATION, ISRAEL NATIONAL ACADEMY OF

SCIENCE. "Molecular characterization and heterologous expression of the

cellulosome complex from Clostridium thermocellum. 1995-1997: $130,000. Y.

Shoham, E. Bayer and E. Morag.

7. ISRAEL MINSTRY OF AGRICULTURE. "Bioconversion of essential oils

components to natural flavors and fragrances" 1996-1999: NS 210,000. Y. Shoham and

U. Ravid.

8. ISRAEL MINISTRY OF SCIENCE. "Rational design of thermostable hemicellulases

for enhanced stability and novel applications" 1996-1998: $210,000. Y. Shoham, T.

Baasov and G. Shoham.

9. UNITED STATES-ISRAEL BINATIONAL SCIENCE FOUNDATION (BSF). Cell

density-dependent (quorum-sensing) activation of extracellular enzymes in Bacillus

stearothermophilus 1997-1999: $ 110,000. Y. Shoham and A. L. Sonenshein.

10. THE BASIC RESEARCH FOUNDATION, ISRAEL NATIONAL ACADEMY OF

SCIENCE. "Functional components of the cellulosome complex from Clostridium

thermocellum: molecular characterization and heterologous expression. 1998-

2000: $ 200,000. Y. Shoham and E. Bayer.

11. NIEDERSACHSEN - ISRAEL RESEARCH COOPERATION PROGRAM.

"Structure and function of specific cellulose- and chitin binding proteins" 1999 - 2000:

DM 180,000. Y. Shoham and H. Schrempf.

12. THE ISRAEL SCIENCE FOUNDATION Protein engineering of hemicellulases for

novel properties and applications 2000-2004: $ 240,000. G. Shoham and Y. Shoham.

13. ISRAEL MINESTRY OF INDUSTRY AND TRADE-MAGNETON PROGRAM

"Generation of a novel lipase" 2000-2002: $ 350,000. Y. Shoham and Enzymotech

Ltd.

14. THE ISRAEL SCIENCE FOUNDATION "The regulation of the cellulosomal complex

in Clostridium thermocellum" 2001-2004: $ 291,000.

15. ALIS SCHUSTER FUND "Recombinant enzyme complexes" 2001: $11,000

16. AFIRST (France Israel research association) "Engineering glycoside hydrolases for

oligosaccharide synthesis" 2002-2003: $110,000, Y. Shoham and B. Henrissat.

17. RUBIN SCIENTIFIC AND MEDICAL RESEARCH FUND "Structure function

studies on KDO8P synthase using solid phase NMR and X-ray crystalography" 2002:

$15,000, T. Baasov and Y. Shoham

18. GERMAN ISRAEL FOUNDATION FOR SCIENTIFIC RESEARCH &

DEVELOPMENT (GIF) Structure-function of carbohydrate active enzymes: the

design of novel functionalities 2004-2007 EURO 475,000, Y. Shoham (coordinator),

G. Shoham, D. Schomburg, T. Baasov

19. THE ISRAEL SCIENCE FOUNDATION "Regulation and functional analysis of the

xylan utilization elements in Geobacillus stearothermophilus" 2005-2009: $260,000.

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20. NIEDERSACHSEN - ISRAEL RESEARCH COOPERATION PROGRAM.

"Genome-wide gene expression analysis of the cellulose degrading systenm in

Clostridium thermocellum". 2006-2008. EURO 250,000, Y. Shoham, S. Yaron, F.

Stahl, T.H. Scheper.

21. UNITED STATES-ISRAEL BINATIONAL SCIENCE FOUNDATION (BSF).

Regulation and functional analysis of cellulose utilization elements in Clostridium

thermocellum. 2006 2010. $200,000. Y. Shoham and A. L. Sonenshein.

22. GAN SHMUEL INDUSTRIES. Enzymatic treatments of juice. 2006-2008: $200,000.

23. THE ISRAEL SCIENCE FOUNDATION "Competing for carbon in the soil: quorum

sensing regulation of the extracellular xylanase in Geobacillus stearothermophilus"

2010-2013: $300,000.

24. MAADANEY YEHIAM LTD. "Developing powder mold starter-cultures" 2010:

$100,000

EQUIPMENT GRANTS

1. THE BASIC RESEARCH FOUNDATION, ISRAEL NATIONAL ACADEMY OF

SCIENCE. FPLC system. 1995, $ 30,000.

2. THE BASIC RESEARCH FOUNDATION, ISRAEL NATIONAL ACADEMY OF

SCIENCE. Microcalorimter VP-DSC and VP-ITC systems. 1999, $ 80,000.

3. THE ISRAEL SCIENCE FOUNDATION, Continuous fermentor, 2001, $ 15,000.

4. THE ISRAEL SCIENCE FOUNDATION, Stopped-flow absorbance, fluorescence and

circular dichroism spectrometer, 2004, $70,000.

5. THE ISRAEL SCIENCE FOUNDATION, Real Time PCR, 2005, $ 30,000.

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PUBLICATIONS

Theses

M.Sc. Emulsan Depolymerase, 1982. Tel Aviv University

Ph.D. Studies on the Stability of Plasmids in Bacillus subtilis, 1988. M.I.T.

Refereed papers in professional journals

A. Published papers

1. Shoham, Y., M. Rosenberg, and E. Rosenberg. Bacterial degradation of emulsan.

Appl. Environ. Microbiol. 46:573-579, 1983.

2. Shoham, Y., and E. Rosenberg. Enzymatic depolymerization of emulsan. J.

Bacteriol. 156:161-167, 1983.

3. Pinas, O., Y. Shoham, E. Rosenberg, and D. Gutnick. Unmasking of surface

components by removal of cell-associated emulsan from Acinetobacter calcoaceticus

RAG-1. Appl. Microbiol. Biotechnol. 28:93-99, 1988.

4. Shoham, Y., and A.L. Demain. Effect of medium composition on the maintenance of

a recombinant plasmid in Bacillus subtilis. Enzyme Microb. Technol. 12:330-336,

1990.

5. Shoham, Y., and A.L. Demain. Stabilization of a plasmid-encoded LacZ phenotype in

Bacillus subtilis. Curr. Microbiol. 20:373-379, 1990.

6. Shoham, Y., and A.L. Demain. Kinetics of loss of a recombinant plasmid in

Bacillus subtilis. Biotechnol. Bioeng. 37:927-935, 1991.

7. Shoham, Y., E. Israeli, A.L. Sonenshein, and A.L. Demain. Inhibition of growth of

Bacillus subtilis by recombinant plasmid pCED3. Arch. Microbiol. 156:204-212, 1991.

8. Shoham, Y., G. Stephanopoulos, and A.L. Demain. Effects of the -lactamase gene

orientation of the kanamycin-resistance gene in plasmid pCED3 on the growth of

Bacillus subtilis. J. Ferment. Bioeng. 72:244-248, 1991.

9. Neutra R., B-Z. Levi and Y. Shoham. Optimization of protein-production by the

baculovirus expression vector system in shake flasks. Appl. Microbiol. Biotechnol.

37:74-78, 1992.

10. Shoham, Y., Z. Schwartz, A. Khasin, O. Gat, Z. Zosim and E. Rosenberg.

Delignification of wood pulp by a thermostable xylanase from Bacillus

stearothermophilus strain T-6. Biodegradation. 3:207-218, 1992.

11. Khasin, A., I. Alchanati and Y. Shoham. Purification and characterization of

xylanase from Bacillus stearothermophilus T-6. Appl. Enviorn. Microbiol

59:1725-1730, 1993.

12. Lundgren, K. R., L. Bergkvist, S. Hogman, H. Joves, G. Eriksson, T. Bartfai, J. van der

Laan, E. Rosenberg and Y. Shoham. Bleaching softwood pulp with Korsnas

thermostable and alkaline stable xylanase T6 and lignox. Svensk

Papperstidning/nordisk cellulosa 7:40-42, 1993.

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13. Shoham, Y., Z. Zosim and E. Rosenberg. Partial decolorization of Kraft pulp at high

temperature and at high pH values with an extracellular xylanase from Bacillus

stearothermophilus. J. Biotechnol. 30:123-131, 1993.

14. Bezalel, L., Y. Shoham and E. Rosenberg. Characterization and delignification

activity of a thermostable -L-arabinofuranosidase from Bacillus stearothermophilus.

Appl. Microbiol. Biotechnol. 40:57-62, 1993.

15. Lundgren, K. R., L. Bergkvist, S. Hogman, H. Joves, G. Eriksson, T. Bartfai, J. van der

Laan, E. Rosenberg and Y. Shoham. TCF mill trial on softwood pulp with Korsnas

thermostable and alkaline stable xylanase T-6. FEMS Microbiol. Rev. 13:365-368,

1994.

16. Gat O., A. Lapidot, I. Alchanati, C. Regueros and Y. Shoham. Cloning and DNA

sequence of the gene coding for Bacillus stearothermophilus T-6 xylanase. Appl.

Enviorn. Microbiol. 60:1889-1896, 1994.

17. Handelsman, T., and Y. Shoham. Production and characterization of an extracellular

thermostable lipase from a Bacillus sp. J. Gen. Appl. Microbiol. 40:435-443, 1994.

18. Gilead S. and Y. Shoham. Purification and characterization of a -L

arabinofuranosidase from Bacillus stearothermophilus T-6. Appl. Enviorn.

Microbiol. 61:170-174, 1995.

19. Yaron, S., E. Morag, E.A. Bayer, R. Lamed and Y. Shoham. Expression, purification

and subunit-binding properties of cohesins 2 and 3 of the Clostridium thermocellum

cellulosome. FEBS 360:121-124, 1995.

20. Morag E., A. Lapidot, D. Govorko, R. Lamed, M. Wilchek, E. A. Bayer and Y.

Shoham. Expression, purification and characterization of the cellulose-binding

domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum.

Appl. Enviorn. Microbiol. 61:1980-1986, 1995

21. Fishman A., Z. Berk, and Y. Shoham. Large scale purification of xylanase T-6.

Appl. Microbiol. Biotechnol. 44: 88-93, 1995.

22. Tormo, J., R. Lamed, A. J. Chirino, E. Morag, E. A. Bayer, Y. Shoham, and T. A.

Steitz. Crystal structure of a bacterial family-III cellulose-binding domain: a general

mechanism for attachment to cellulose. EMBO J. 15: 5739-5751, 1996.

23. Lapidot, A., A. Mechaly, and Y. Shoham. Overexpression and single step

purification of a thermostable xylanase from Bacillus stearothermophilus T-6. J.

Biotechnol. 51:259-264, 1996.

24. Morag, E., S. Yaron, R. Lamed, Y. Shoham, and E. A. Bayer. Dissociation of the

cellulosome of Clostridium thermocellum under nondenaturing conditions. J.

Biotechnol. 51:235-242, 1996.

25. Yaron, S., L. J. W. Shimon, F. Frolow, R. Lamed, E. Morag, Y. Shoham, and E. A.

Bayer. Expression, purification, and crystallization of a cohesin domain from the

cellulosome of Clostridium thermocellum. J. Biotechnol. 51:243-249, 1996.

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Shimon, L. J.W., F. Frolow, S. Yaron, R. Lamed, E. Morag, E. A. Bayer, and Y.

26.

Shoham. Crystallization and preliminary X-ray analysis of a cohesin domain of the

cellulosome from Clostridium thermocellum. Acta Crystallogr. D. 53:114-115, 1997.

27. Shimon, L. J.W., E. A. Bayer, E. Morag, R. Lamed, S. Yaron, Y. Shoham, and F.

Frolow. Three-dimensional crystal structure of a cohesin domain of the cellulosome

from Clostridium thermocellum. Structure 5:381-390, 1997.

28. Teplitsky, A., H. Feinberg, R. Gilboa, A. Lapidot, A. Mechaly, V. Stojanoff, M. Capel,

Y. Shoham, and G. Shoham. Crystallization and preliminary crystallographic

analysis of the thermostable, alkaline tolerant xylanase from Bacillus

stearothermophilus T-6. Acta Crystallogr. D. 53:608-611, 1997.

29. Pages, S., A. Belaich, J.P. Beleich, E. Morag, R. Lamed, Y. Shoham, and E. A. Bayer.

Species-specificity of the cohesin-dockerin interaction between Clostridium

thermocellum and Clostridium cellulolyticum: predication of specificity determinants

of the dockerin domain. Proteins 29:517-527, 1997.

30. Mechaly, A., V. Belakhov, Y. Shoham, and T. Baasov. An efficient chemical-

enzymatic synthesis of 4-nitrophenyl -xylobioside: a chromogenic substrate for

xylanases. Carbohydr. Res. 304:111-115, 1997.

31. Bayer, E. A., H. Chanzy, R. Lamed, and Y. Shoham. Cellulose, cellulases and

cellulosomes. Curr. Opin. Struct. Biol. 8:548-557, 1998.

32. Bayer, E. A., L.J.W. Shimon, Y. Shoham, and R. Lamed. Cellulosomes-structure and

ultrastructure. J. Structural Biology 124:221-234, 1998.

33. Teplitsky, A., S. Shulami, S. Moryles, G. Zaide, Y. Shoham, and G. Shoham.

Crystalization and preliminary X-ray analysis of -D-glucuronidase from Bacillus

stearothemophilus T-6. Acta Crystallogr. D55:869-872, 1999.

34. Boisset, C., Chanzy, H., Henrissat, B., Lamed, R., Shoham, Y. and Bayer, E. A.

Digestion of crystalline cellulose substrates by the Clostridium thermocellum

cellulosome: Structural and morphological aspects. Biochem. J. 340:829-835, 1999.

35. Shoham, Y., R. Lamed, and E.A. Bayer. The cellulosome concept as an efficient

microbial strategy for the degradation of insoluble polysaccharides. Trends in

Microbiol. 7:275-281, 1999.

36. Ding, S-Y., E.A. Bayer, D. Steiner, Y. Shoham, and R. Lamed. A novel cellulosomal

scaffoldin from Acetivibrio cellulolyticus which contain a Family 9 glycosyl hydrolase.

J. Bacteriol. 181:6720-6729, 1999.

37. Berdichevsky, Y., R. Lamed, D. Frenkel, U. Gophna, E. A. Bayer, S. Yaron, Y.

Shoham, and I. Benhar. Matrix-assisted refolding of single-chain Fv -cellulose

binding domain fusion proteins. Protein Express. Purif. 17:249-259, 1999.

38. Shulami, S., O. Gat, A.L. Sonenshein, and Y. Shoham. The glucuronic acid utilization

gene cluster from Bacillus stearothermophilus T-6. J. Bacteriol. 181:3695-3704, 1999

39. Teplitsky, A., S. Shulami, S. Moryles, Y. Shoham, and G. Shoham.

Crystallization and preliminary X-ray analysis of an intracellular xylanase from

Bacillus stearothermophilus T-6. Acta Crystallogr. D56, 181-184, 2000.

11

40. Kaustov, L., S. Kababya, D. Schoucheng, T. Baasov, S. Gropper, Y. Shoham, and

A. Schmidt. Direct identification of enzyme active site residues by solid phase

REDOR NMR: Application to KDO8P syntase. J. Am. Chem. Soc 122:2649-2650,

2000.

41. Mechaly, A., S. Yaron, H-P. Fierobe, A. Belaich, J-P. Belaich, R. Lamed, Y. Shoham,

and E. A. Bayer. Cohesin-dockerin recognition in cellulosome assembly: experiment

versus hypothesis. Proteins 39:170-177, 2000.

42. Kauffmann, C., O. Shoseyov, E. A. Bayer, R. Lamed, Y. Shoham, and R. T.

Mandelbaum. A novel methodology for enzymatic removal of atrazine from water by

CBD-fusion protein immobilized on cellulose. Environ. Sci. Technol. 34:1292-1296,

2000

43. Ding, S-Y., E.A. Bayer, D. Steiner, Y. Shoham, and R. Lamed. A scaffoldin of the

Bacteroides cellulosolvens cellulosome that contains eleven type-II cohesins. J.

Bacteriol. 182:4915-4925, 2000.

44. Shimoni, E., U. Ravid, and Y. Shoham. Isolation of a Bacillus sp. capable of

transforming isoeugenol to vanillin. J. Biotechnol 78:1-9, 2000.

45. Mechaly, A., A. Teplitsky, V. Belakhov, T. Baasov, G. Shoham and Y. Shoham.

Overproduction and characterization of seleno-methionine xylanase T-6. J.

Biotechnol. 78:83-86, 2000

46. Linda J.W. Shimon, Sandrine Pag s, Anne Belaich, Jean-Pierre Belaich, Edward A.

Bayer, Raphael Lamed, Yuval Shoham, and Felix Frolow. Crystal structure of a

Family-IIIa scaffoldin CBD from the cellulosome of Clostridium cellulolyticum at 2.2

resolution. Acta Cryst D56:1560-1568, 2000.

47. Kaustov, L., Kababya, S., Du, S., Baasov, T., Gropper, S., Shoham, Y., and Schmidt,

A. Structural and mechanistic investigation of 3-deoxy-D-manno-octulosonate-8-

phosphate syntahase by solid-state REDOR NMR. Biochemistry 39:148**-*****,

2000.

48. Lewinson, E., I. Ziv-Raz, N. Dudai, Y. Tadmor, E. Lastochkin, O. Larkov, D.

Chaimovitsh, U. Ravid, E. Putievsky, E. Pichersky, and Y. Shoham. Biosysnthesis of

estragole and methyl-eugenol in sweet basil (Ocimum basilicum L): Developmental

and cghemotypic association of allylphenol O-methyltransferase activities. Plant

Science 160:27-35, 2000

49. Lamed, R., Kenig, R., Morag, E., Yaron, S., Shoham, Y. and Bayer, E. A.

Nonproteolytic cleavage of aspartyl proline bonds in the cellulosomal scaffoldin

subunit from Clostridium thermocellum. Appl. Biochem. Biotechnol. 90:67-74, 2001.

Shi-You Ding, Marco T. Rincon, Raphael Lamed, Jennifer C. Martin, Sheila I.

50.

McCrae, Vincenzo Aurilia, Yuval Shoham, Edward A. Bayer, and Harry J. Flint.

Cellulosomal scaffoldin-like proteins from Ruminococcus flavefaciens. J. Bacteriol.

183:1945-1953, 2001

51. Mechali, Adva, Henri-Pierre Fierobe, Anne Belaich, Jean-Pierre Belaich, Raphael

Lamed, Yuval Shoham, and Edward, A. Bayer. Coheisn-dockerin interaction in

cellulosome assembly: a single hydroxyl group of a dockerin domain distinguish

12

between non-recognition and high -affinity recognition. J. Biol. Chem. 276: 9883-

9888, 2001.

52. Asojo Oluwatoyn, Jonathan Friedman, Noam Adir, Valery Belakhov, Yuval

Shoham, and Timor Baasov. Crystal structure of KDO8P in its binary complexes

with the substrate PEP and with a mechanism-based inhibitor. Biochemistry

40:6326-6334, 2001.

53. Belakov Valery, Mark Botoshansky, Evgenia Dor, Joseph Hershenhorn, Tsfrir

Bravman, Mirit Kolog, Gil Shoham, Yuval Shoham, and Timor Baasov. A family

of thiomercuric derivatives of sugars: synthesis, fungicidal/herbicidal activity, and

application to the X-ray structure determination of the corresponding enzymes. Isr.

J. Chem. 40:177-188, 2001

54. Bravman, Tsfrir, Gennady Zolotnitsky, Smadar Shulami, Valery Belakhov, Dmitry

Solomon, Timor Baasov, Gil Shoham, and Yuval Shoham. Stereochemistry of family

52 glycosyl hydrolases: a -xylosidase from Bacillus stearothermophilus T-6 is a

retaining enzyme. FEBS Lett. 495:39-43, 2001

55. Henri-Pierre Fierobe, Adva Mechaly, Chantal Tardif, Anne Belaich, Raphael Lamed,

Yuval Shoham, Jean-Pierre Belaich, and Edward A. Bayer. Design and production

of active cellulosome chimeras: selective incorporation of dockerin-containing

enzymes into defined functional complexes. J. Biol. Chem. 276:212**-*****, 2001.

56. Zaide Galia, Dalia Shallom, Smadar Shulami, Gennady Zolotnitsky, Gali Golan, Anna

Teplitsky, Timor Baasov, Gil Shoham, and Yuval Shoham. Biochemical

characterization and identification of catalytic residues in -glucuronidase from

Bacillus stearothermophilus T-6. Eur. J. Biochem. 268:3006-3016, 2001.

57. Bravman, Tsfrir, Adva Mechaly, Smadar Shulami, Valery Belakhov, Timor Baasov,

Gil Shoham, and Yuval Shoham. Glutamic acid 160 is the acid-base catalyst of -

xylosidase from Bacillus stearothermophilus T-6: a family 39 glycoside hydrolase.

FEBS Lett. 495:115-119, 2001.

58. Shallom Dalia, Valery Belakhov, Dmitry Solomon, Sara Gilead-Gropper, Timor

Baasov, Gil Shoham, and Yuval Shoham The identification of the acid-base catalyst

of alpha-arabinfuranosidase from Bacillus stearothermophilus T-6, a family 51

glycoside hydrolase. FEBS Lett. 514:163-167, 2002.

59. Shimoni Eyal, Timor Baasov, Uzi Ravid, and Yuval Shoham. The trans-anethole

degradation pathway in an Arthrobacter sp. J. Biol. Chem. 277:118**-*****, 2002.

60. Dalia Shallom, Valery Belakhov, Dmitry Solomon, Gil Shoham, Timor Baasov, and

Yuval Shoham. Detailed kinetic Analysis and identification of the nucleophile in -

L-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51

glycoside hydrolase, J. Biol. Chem 277:436**-*****, 2002.

61. Henri-Pierre Fierobe, Edward A. Bayer, Chantal Tardif, Mirjam Czjzek, Adva

Mechaly, Anne Belaich, Raphael Lamed, Yuval Shoham, and Jean-Pierre Belaich.

Degradation of cellulose substrates by cellulosome chimeras: substrate targeting versus

proximity of enzyme components. J. Biol. Chem. 277:496**-*****, 2002.

62. Rachel Gilad, Larisa Rabinovich, Sima Yaron, Edward A. Bayer, Raphael Lamed,

Harry J. Gilbert and Yuval Shoham. CelI, a non-cellulosomal family-9 enzyme from

13

Clostridium thermocellum, is a processive endoglucanase that degrades crystalline

cellulose. J. Bacteriol. 185:391-398, 2003.

63. Marco T. Rincon, Shi You Ding, Martin, Aurilia, Raphael Lamed, Yuval Shoham,

Edward A. Bayer, and Harry J. Flint. Novel organization and divergent dockerin

specificities in the cellulosome system of Ruminococcus flavefaciens. J. Bacteriol.

185:703-713, 2003.

64. Kaustov Lilia, Shifi Kababya, Valery Belakhov, Timor Baasov, Yuval Shoham, and

Asher Schmidt. Inhibition mode of a bi-substrate inhibitor of KDO8P synthase: A

frequency-selective REDOR solid state and solution NMR characterization. J. Am.

Chem. Soc. 125:4662-4669, 2003.

65. Klaus H vel, Dalia Shallom, Karsten Niefind, Timor Baasov, Gil Shoham, Yuval

Shoham and Dietmar Schomburg. Crystallization and preliminary X-ray analysis of a

family 51 glycoside hydrolase, the -L-arabinofuranosidase from Geobacillus

stearothermophilus T-6. Acta Crystallogr. D59:913-915, 2003.

66. Tali W. Dror, Ely Morag, Adi Rolider, Edward A. Bayer, Raphael Lamed and Yuval

Shoham. Regulation of the cellulosomal celS (cel48A) gene of Clostridium

thermocellum is growth-rate dependent. J. Bacteriol. 185:2042-3048, 2003.

67. Dallia Shallom and Yuval Shoham. Microbial Hemicellulases. Curr. Opin.

Microbiol.6:219-228, 2003.

68. Tsafrir Bravman, Valery Belakhov, Dmitry Solomon, Gil Shoham, Bernard Henrissat,

Timor Baasov and Yuval Shoham. Identification of the catalytic residues in family 52

glycoside hydrolase, a -xylosidase from Geobacillus stearothermophilus T-6. J. Biol.

Chem. 278:267**-*****, 2003.

69. Qi Xu, Shi-You Ding, Wenchen Gao, Rina Kenig, Yuval Shoham, Edward A. Bayer,

and Raphael Lamed. The cellulosome system of Acetivibrio cellulolyticus includes a

novel type of adaptor protein and a cell-surface anchoring protein. J. Bacteriol.

185:4548-4557, 2003.

70. Eyal Shimoni, Timor Baasov, Uzi Ravid, and Yuval Shoham. Biotransformation of

propenylbenzenes by an Arthrobacter sp. and its t-anetole blocked mutants. J.

Biotechnol. 105:61-70, 2003.

71. Tali W. Dror, Adi Rolider, Edward A. Bayer, Raphael Lamed and Yuval Shoham.

Regulation of expression of scaffoldin-related genes in Clostridium thermocellum. J.

Bacteriol. 185:5109-5116, 2003.

72. Tsafrir Bravman, Gennady Zolotnitsky, Valery Belakhov, Timor Baasov, Gil Shoham,

Bernard Henrissat, and Yuval Shoham. Detailed kinetic analysis of a family 52

glycoside hydrolase: a -xylosidase from Geobacillus stearothermophilus.

Biochemistry 42:105**-*****, 2003.

73. Klaus H vel, Dalia Shallom, Karsten Niefind, Valery Belakhov, Gil Shoham, Timor

Baasov, Yuval Shoham, and Dietmar Schomburg. Crystal structure and snapshots

along the reaction pathway of -L-arabinofuranosidase, a family 51 glycosidase.

EMBO J. 22:4922-4932, 2003.

74. Shulami Smadaer, Orit Yaniv, Yuval Shoham, and Timor Baasov. Cloning,

expression and biochemical characterization of 3-deoxy-D-manno-2-octulosonate-8-

14

phosphate (KDO8P) synthase from the hyperthermophilic bacterium Aquifex

pyrophilus. Extremophiles 7:471-481, 2003.

75. Valery Belakhov, Ekaterina Dovgolevsky, Emilia Rabkin, Smadar Shulami, Yuval

Shoham, and Timor Baasov. Synthesis and biological activity of a mechanism-based

inhibitor of KDO8P synthase. Carbohydr. Res 339:385-392. 2004.

76. Gali Golan, Dalia Shallom, Anna Teplitsky, Galia Zaide, Smadar Shulami, Timor

Baasov, Vivian Stojanoff, Andy Thompson, Yuval Shoham, and Gil Shoham. Crystal

structures of Geobacillus stearothermophilus a-glucuronidase complexed with its

substrate and products: mechanistic implications. J. Biol.Chem. 279:3014-3024. 2004.

77, Qi Xu, Edward A. Bayer, Milana Goldman, Rina Kenig, Yuval Shoham and

Raphael Lamed. Architecture of the Bacteroides cellulosolvens cellulosome:

description of a cell-surface anchoring scaffoldin and a Family 48 cellulase. J.

Bacteriol. 186:968-977. 2004.

78. Maya Bar, Gali Golan, Magda Nechama, Gennady Zolotnitsky, Yuval Shoham, and

Gil Shoham. A new crystal form of XT6, enables a significant improvement of its

diffraction quality and resolution. Acta Crystallogr. D60:545-549. 2004.

79. Mirjam Czjzek, Tsafrir Bravman, Bernard Henrissat, Yuval Shoham. Crystallization

and preliminary X-ray analysis of family 39 -D-xylosidase from Geobacillus

stearothermophilus T-6. Acta Crystallogr. D60:583-585. 2004.

80. A. Teplitsky, A. Mechaly, V. Stojanoff, G. Sainz, G. Golan, H. Feinberg, R. Gilboa1,

V. Reiland, G. Zolotnitsky, D. Shallom, A. Thompson, Y. Shoham & G. Shoham.

Structure determination of the extracellular xylanase from Geobacillus

stearothermophilus by selenomethionyl MAD phasing. Acta Crystallogr. D60:836-848.

2004.

81. Edward A. Bayer, Jean-Pierre Belaich, Yuval Shoham, and Raphael Lamed. The

cellulosomes: multi-enzyme machines for degradation of plant cell wall

polysaccharides. Ann. Rev. Microbiol. 58:521-554. 2004

82. Yifat Fundoiano-Hershcovitz, Larisa Rabinovitch, Yael Langut, Vera Reiland Gil

Shoham, and Yuval Shoham. Identification of the catalytic residues in the double

zinc aminopeptidase from Streptomyces griseus. FEBS Lett. 571:192-196, 2004

83. Mirjam Czjzek, Tsafrir Bravman, Bernard Henrissat, and Yuval Shoham.

Crystallization and preliminary crystallographic analysis of a thermostable family 52

-D-xylosidase from Geobacilluss stearothermophilus T-6. Acta Crystallogr.

D60:1461-1463, 2004.

84. Gennady Zolotnitsky, Uri Cogan, Noam Adir, Vered Solomon, Gil Shoham, and Yuval

Shoham. Mapping glycoside hydrolase substrate subsite by isothermal titration

calorimetry. Proc. Natl. Acad. Sci. USA 101:112**-*****, 2004.

85. Qi Xu, Yoav Barak, Rina Kenig, Yuval Shoham, Edward A. Bayer, and Raphael

Lamed. A novel Acetivibrio cellulolyticus anchoring scaffoldin that bears divergent

cohesins. J. Bacteriol. 186:5782-5789, 2004.

86. Tal Handelsman, Yoav Barak, David Nakar, Adva Mechaly, Raphael Lamed, Yuval

Shoham, and Edward A. Bayer. Cohesin-dockerin interaction in cellulosome

15

assembly: A single Asp-to-Asn mutation disrupts high-affinity cohesin-dockerin

binding. FEBS Lett. 572:195-200, 2004.

87. Reiland, V., R. Gilboa, A. Spungin-Bialik, D. Schomburg, Y. Shoham, S. Blumberg,

and G. Shoham. Binding of inhibitory aromatic amino acids to Streptomyces griseus

aminopeptidase. Acta Crystallogr. D60:1738-1746, 2004.

88. Dalia Shallom, Gali Golan, Gil Shoham and Yuval Shoham. Effect of dimer

dissociation on activity and thermostability of the -glucuronidase from Geobacillus

stearothermophilus: dissecting the different oligomeric forms of family 67 glycoside

hydrolases. J. Bacteriol 186:6928-6937, 2004.

89. David Nakar, Tal Handelsman, Yuval Shoham, Henri-Pierre Fierobe, Jean-Pierre

Belaich, Ely Morag Raphael Lamed and Edward A. Bayer. Pinpoint mapping of

recognition residues on the cohesin surface by progressive homologue swapping. J.

Biol. Chem. 279:428**-*****, 2004.

90. Smadar Shulami, Cristina Furdui, Noam Adir, Yuval Shoham, Karen S. Anderson,

and Timor Baasov. A reciprocal single mutation affects the metal requirement of 3-

deoxy-D-manno-2-octulosonate-8-phosphate (KDO8P) synthases from Aquifex

pyrophilus and Escherichia coli. J. Biol. Chem. 279:451**-*****, 2004.

91. Or Dgany, Ana Gonzalez, Oshrat Sofer, Wangxia Wang, Gennady Zolotnitsky, Amnon

Wolf, Yuval Shoham, Arie Altman, Sharon G. Wolf, Oded Shoseyov, and Orna

Almog. The structural basis of the thermostability of SP1, a novel plant (Populus

tremula) boiling stable protein. J. Biol. Chem. 279:51516 - 51523, 2004

92. Reiland, V., Fundoiano-Hershcovitz, Y., Golan, G., Gilboa, R., Shoham, Y. and

Shoham, G. Preliminary crystallographic characterization of SGAP, an extracellular

aminopeptidase from Bacillus subtilis, Acta Cryst. D60:2371-2376, 2004.

93. Dalia Shallom, Maya Leon, Tsafrir Bravman, Alon Ben-David, Galia Zaide, Valery

Belakhov, Gil Shoham, Dietmar Schomburg, Timor Baasov, and Yuval Shoham.

Biochemical characterization and identification of the catalytic residues of a family 43

-D-xylosidase from Geobacillus stearothermophilus T-6. Biochemistry 44:387-397,

2005.

94. Yifat Fundoiano-Hershcovitz, Larisa Rabinovitch, Smadar Shulami, Vera Reiland, Gil

Shoham, and Yuval Shoham. The ywad gene from Bacillus subtilis encodes for a

double-zinc aminopeptidase. FEMS Lett. 243:157-163, 2005.

95. Tali W. Dror, Adi Rolider, Edward A. Bayer, Raphael Lamed and Yuval Shoham.

Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs

from that of a prominent cellulosomal xylanase. J. Bacteriol. 187:2261-2266, 2005.

96. Michal Soreni, Sivan Yogev, Elizaveta Kossoy, Yuval Shoham, and Ehud Keinan.

Parallel biomolecular computation with advanced finite automata. J. Am. Chem. Soc.

127:3935-3943, 2005.

97. Yoav Barak, Tal Handelsman, David Nakar, Adva Mechaly, Raphael Lamed, Yuval

Shoham, and Edward A. Bayer. Matching fusion-protein systems for affinity analysis

of two interacting families of proteins: The cohesin-dockerin interaction. J. Mol.

Recognit. 18:491-501, 2005.

16

98. Mirjam Czjzek, Alon Ben David, Tsafrir Bravman, Gil Shoham, Bernard Henrissat,

and Yuval Shoham. Enzyme-substrate complex structures of a GH39 -xylosidase

from Geobacillus stearothermophilus. J. Mol. Biol. 353:838-846, 2005.

99. Christian Brux, Karsten Niefind, Alon Ben-David, Maya Leon, Gil Shoham, Yuval

Shoham, and Dietmar Schomburg. Crystallization and preliminary crystallographic

analysis of a family 43 -D-xylosidase from Geobacillus stearothermophilus T-6.

Acta Cryst F61:1054-1057, 2005

100. Sadanari Jindou, Qi Xu, Rina Kenig, Michal Shulman, Yuval Shoham, Edward Bayer,

and Raphael Lamed. Novel architecture of family-9 glycoside hydrolases identified in

cellulosomal enzymes of Acetivibrio cellulolyticus and Clostridium thermocellum.

FEMS Lett. 254:308-316, 2006.

101. Christian Br x, Alon Ben-David, Dalia Shallom-Shezifi, Maya Leon, Karsten Niefind,

Gil Shoham, Yuval Shoham, Dietmar Schomburg. The structure of an inverting GH43

-xylosidase from Geobacillus stearothermophilus with its substrate reveals the role of

the three catalytic residues. J. Mol. Biol. 359:97-109, 2006.

102. Jonathan Caspi, Diana Irwin, Raphael Lamed, Yuval Shoham, Henri-Pierre Fierobe,

David B. Wilson, and Edward A. Bayer. Thermobifida fusca family-6 cellulases as

potential designer cellulosome components. Biocatalysis and Biotransformation 24:3-

12, 2006.

103. Smadar Shulami, Galia Zaide, Gennady Zolotnitsky, Yael Langut, Geoff Feld,

Abraham L. Sonenshein and Yuval Shoham. A two-component system regulates the

expression of an ABC transporter for xylo-oligosaccharides in Geobacillus

stearothermophilus. Appl. Enviorn. Microbiol. 73:874-884, 2007.

104. Elizaveta Kossoy, Noa Lavid, Michal Soreni, Yuval Shoham and Ehud Keinan. A

programmable bio-molecular computing machine with bacterial phenotype output.

ChemBioChem. 8:1255-1260, 2007.

105. Solomon, V., A. Teplitsky, S. Shulami, G. Zolotnitsky, Y. Shoham, and G. Shoham.

Structure-specificity relationships of an intracellular xylanase from Geobacillus

stearothermophilus. Acta Cryst. D63:845-859, 2007.

106. Yifat Fundoiano-Hershcovitz, Rotem Gilboa, Vera Reiland, Gil Shoham and Yuval

Shoham. Catalytic mechanism of SGAP, a double-zinc aminopeptidase from

Streptomyces griseus. FEBS J. 274:3864 3876, 2007.

107. Alon Ben-David, Tsafrir Bravman, Yael S. Balazs, Mirjam Czjzek, Dietmar

Schomburg, Gil Shoham, and Yuval Shoham. Glycosynthase activity of Geobacillus

stearothermophilus GH52 -xylosidase: efficient synthesis of xylooligosaccharides

from -D-xylopyranosyl fluoride via a conjugated reaction. ChemBioChem 8:2145-

2151, 2007.

108. Alon Karpol, Yoav Barak, Raphael Lamed, Yuval Shoham and Edward A. Bayer.

Functional asymmetry in cohesin binding belies inherent symmetry of the dockerin

module: Insight into cellulosome assembly revealed by systematic mutagenesis.

Biochem. J. 410:331-338, 2008

109. Haimovitz, Rachel, Yoav Barak, Ely Morag, Yuval Shoham, Milana Voronov-

Goldman, Raphael Lamed, and Edward A. Bayer. Cohesin-dockerin microarray:

17

diverse specificities between two complementary families of interacting protein

modules. Proteomics. 8:968-979, 2008

110. Alon Ben-David, Gil Shoham, and Yuval Shoham. A universal screening assay for

glycosynthases: Directed evolution of glycosynthase XynB2(E335G) suggests a

general path to enhance activity. Chemistry & Biology 15:546-551, 2008.

111. Dan Goldman, Noa Lavid, Alon Schwartz, Gil Shoham, Dganit Danino, and Yuval

Shoham. Two active forms of Zymomonas mobilis levansucrase: an ordered

microfbril strcture of the enzyme promotes levan polymerization. J. Biol. Chem.

283:322**-*****, 2008.

112. Yakir Nataf, Sima Yaron, Frank Stahl, Raphael Lamed, Edward A. Bayer, Thomas-

Helmut Scheper, Abraham L. Sonenshein, and Yuval Shoham. Cellodextrin and

laminaribiose ABC transporters in Clostridium thermocellum. J. Bacteriol. 191:203-

209, 2009.

113. Tal Burstein, Michal Shulman, Sadanari Jindou, Svetlana Petkun, Felix Frolow, Yuval

Shoham, Edward A. Bayer, and Raphael Lamed. Physical association of the catalytic

and helper modules of a family-9 glycoside hydrolase is essential for activity. FEBS

Lett. 583:879-884, 2009.

114. Anat Alhassid, Alon Ben-David, Orly Tabachnikov, Dima Libster, Einat Naveh,

Gennady Zolotnitsky, Yuval Shoham, and Gil Shoham. Crystal structure of an

inverting GH43 1,5- -L-arabinanase from Geobacillus stearothermophilus complexed

with its substrate. Biochem. J. 422:73-82, 2009.

115. Johanna-Gabriela Walter, Frank Stahl, Michael Reck, Inka Praulich, Yakir Nataf,

Markus Hollas, Karl Pflanz, Dieter Melzner, Yuval Shoham, Thomas Scheper. Protein

microarrays: Reduced autofluorescence and improved LOD. Eng. Life Sci. 10:103-

108, 2010.

116. Sarah Mora s, Yoav Barak, Jonathan Caspi, Yitzhak Hadar, Raphael Lamed, Yuval

Shoham, David B. Wilson, and Edward A. Bayer. Degradation of a complex

cellulosic substrate by designer cellulosomes: Contribution of a xylan-binding module.

Appl. Enviorn. Microbiol. 76:3787-3796, 2010.

117. Borovok, Ilya, Hamutal Kahel-Raifer, Sadanari Jindou Liat Bahari, Yakir Nataf,

Yuval Shoham, Edward Bayer, Raphael Lamed. Unique set of putative membrane-

associated anti-sigma factors in Clostridium thermocellum suggests a novel

extracellular carbohydrate-sensing mechanism involved in gene regulation. FEMS

Microbiol. Lett. 308:84 93, 2010.

118. Yakir Nataf, Liat Bahari, Hamutal Kahel-Raifer, Ilya Borovok, Raphael Lamed,

Edward A. Bayer, Abraham L. Sonenshein and Yuval Shoham. Clostridium

thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides

via alternative sigma factors. Proc. Natl. Acad. Sci. USA 107:186**-*****, 2010.

119. Liat Bahari, Yuval GIlad, Ilya Borovok, Hamutal Kahel-Raifer, Bareket Dassa, Yakir

Nataf, Yuval Shoham, Raphael Lamed, Edward A. Bayer. Glycoside hydrolases as

components of putative carbohydrate biosensor proteins in Clostridium thermocellum

J. Ind. Microbiol. Biotechnol. (in press).

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120. Sarah Mora sa, Yoav Barak, Jonathan Caspia, Yitzhak Hadar, Raphael Lamed, Yuval

Shoham, David B. Wilson, and Edward A. Bayer. Cellulase-xylanase synergy in

designer cellulosome for enhanced degradation of a complex cellulosic substrate. mBio

(accepted).

121. Svetlana Petkun, Raphael Lamed, Sadanari Jindou, Tal Burstein, Oren Yaniv, Yuval

Shoham, Linda J.W. Shimon, Edward A. Bayer, and Felix Frolow. Reassembly and

co-crystallization of a family 9 processive endoglucanase from its component parts:

Structural and functional significance of the intermodular linker (submitted).

122. znur K kpinar, Johanna-Gabriela Walter, Yuval Shoham, Frank Stahl, and Thomas

Scheper. Aptamer-based downstream processing of His-tagged proteins utilizing

magnetic beads (submitted).

C. Chapters in Books and Other Publictions

1. Rosenberg, E., M. Rosenberg, Y. Shoham, N. Kaplan, and N. Sar. Adhesion and

desorption during growth of Acinetobacter calcoaceticus on hydrocarbons. In: Cohen,

Y., and E. Rosenberg (Eds.). Microbial Mats: Ecological Physiology of Benthic

Microbial Communities. 1988, pp. 219-227. ASM Publication, Washington, D.C.

2. Hogman, S., H. Joves, E. Rosenberg and Y. Shoham. Bleachability improvment of

softwood kraft pulp through treatment with an alkali- and thermostable xylanase. In:

Kuwahara, M., and M. Shimada (Eds.), Biotechnology in Pulp and Paper Industry.

1992, pp. 107-113. Uni Publishers, Tokyo, Japan.

3. Bayer, E.A., E. Morag, M. Wilchek, R. Lamed, S. Yaron, and Y. Shoham.

Cellulosome domains for novel biotechnological application. In: Petersen, S.B., B.

Svensson, and S. Pedersen (eds.), Carbohydrate Bioengineering. 1995, pp. 251-259,

Elsevier Science, Amsterdam.

4. Shimoni, E., U. Ravid, and Y. Shoham. Biotransformation of essential oil

components to natural flavors and fragrances. In: 1996 Proceedings of the

International Conference of Aroma and Essentail Oils. pp. 62-69, Tel Aviv.

5. Bayer, E., E. Morag, Y. Shoham, J. Tormo, and R. Lamed. The cellulosome: A cell-

surface for the adhesion to and degradation of cellulose. In: Fletcher, M., and D.

Savage (Eds.), Molecular and Ecological Diversity of Bacterial Adhesion. 1996. pp.

155-182 Wiley-Liss, Inc, New York.

6. Bayer, E. A., E. Morag, R. Lamed, S. Yaron, and Y. Shoham. Cellulosome structure:

four-pronged attack using biochemistry, molecular biology, crystallography and

bioinformatics, In: M. Claeyssens, K. Piens and W. Nerinckx (Eds.), Carbohydrases

from Trichoderma reesei and other microorganisms. 1998, pp39-67. The Royal

Society of Chemistry, London.

7. Shimoni, E., U. Ravid, and Y. Shoham. Bioconversion of alkene phenols from

aromatic plants to natural aroma compounds. In: K. H. C. Baser and N. Kirimer (eds),

Progress in Essential Oil Research, 1998, pp. 280-294. Anadolu University Press,

Elkisehir.

8. Ziv-Raz, I., E. Lewinson, N. Dudai, U. Ravid, I. Katzir, E. Putievsky, and Y. Shoham.

Methyltransferases involved in the biosythesis of methyl eugenol and methyl chavicol

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in sweet basil. In: K. H. C. Baser and N. Kirimer (eds), Progress in Essential Oil

Research, 1998, pp. 295-302. Anadolu University Press, Elkisehir.

9. Bayer, E. A., Ding, S. Y., Shoham, Y. and Lamed, R. (1999). New perspectives in the

structure of cellulosome-related domains from different species. In (K. Ohmiya, K.

Hayashi, K. Sakka, Y. Kobayashi, S. Karita and T. Kimura, ed.) Genetics,

Biochemistry and Ecology of Cellulose Degradation, pp. 428-436. Uni Publishers Co.,

Ltd., Tokyo, Japan.

10. Bayer, E. A., Ding, S.-Y., Mechaly, A., Shoham, Y. and Lamed, R. (1999). Emerging

phylogenetics of cellulosome structure. In: Recent Advances in Carbohydrate

Bioengineering (H. J. Gilbert, G. J. Davies, B. Henrissat and B. Svensson, eds.), pp.

189-201. The Royal Society of Chemistry, Cambridge.

11. Bayer, E. A., Y. Shoham, and R. Lamed. (2000). The cellulosome an exocellular

organelle for degrading plant cell wall polysaccharides. In: (R. J. Doyle, ed)

Glycomicrobiology. P.387-439 Kluwer Academic/Plenum Publishers, New York.

12. Benhar, I., Tamarkin, A., Marash, L., Berdichevsky, Y., Yaron, S., Shoham,Y.,

Lamed, R. and Bayer, E. A. (2001). Phage display of cellulose binding domains for

biotechnological application. In Glycosyl Hydrolases for Biomass Conversion. ACS

Symposium Series 769 (M. E. Himmel, J. O. Baker and J. N. Saddler, ed.), pp. 168-

189. American Chemical Society, Washington, DC.

13. Bayer, E.A., Shoham, Y., and Lamed, R., Cellulose-decomposing bacteria and their

enzyme systems, M. Dworkin et al., eds., The Prokaryotes: An evolving electronic

resource of the microbiological community 3rd edition (latest update release 3.7,

September 2001), New York, Springer-Verlag, 2000.

14. Shimoni, E., Ravid, U. and Shoham, Y. (2001). Flavor production by

biotransformation. Chimia 54: 28-30 (in Hebrew)

15. Fierobe, H.-P., Mechaly, A., Tardif, C., Belaich, A., Lamed, R., Shoham, Y., Belaich,

J.-P., and Bayer, E. A. (2002) Designer nanosomes: Selective engineering of dockerin-

containing enzymes into chimeric scaffoldins to form defined nanoreactors in

Carbohydrate bioengineering: Interdisciplinary approaches (Teeri, T. T., Svensson, B.,

Gilbert, H. J., and Feizi, T., Eds.), pp. 113-123, The Royal Society of Chemistry,

Cambridge.

16. Kossoy E, Soreni M, Shoham Y, and E. Kinan. (2002) Toward computing machines

made of biomolecules. 6TH WORLD MULTICONFERENCE ON SYSTEMICS,

CYBERNETICS AND INFORMATICS, VOL XVII, PROCEEDINGS -

INDUSTRIAL SYSTEMS AND ENGINEERING III Pages: 340-342 Conference

Information: 6th World Multi-Conference on Systemics, Cybernetics and Informatics

(SCI 2002)/8th International Conference on Information Systems Analysis and

Synthesis (ISAS 2002), JUL 14-18, 2002 ORLANDO, FL

17. Handelsman, T., A. Mechaly, Y. Barak, D. Nakar, R. Lamed, Y. Shoham, and E. A.

Bayer. (2004). Effect of rational mutagenesis of selected cohesin residues on the high-

affinity cohesin-dockerin interaction. In: B. Sahah and K. Hayashi (eds.),

Lignocellulose Biodegradation. ACS Symposium Series 889:194-206. American

Chemical Society, Washington, DC.

18. Bayer, E.A., Nakar, D., Handelsman, T., Mechaly, A., Fierobe, H.-P., Belaich, J. P.,

Barak, Y., Xu, Q., Shoham, Y., & Lamed, R. (2004). Probing the cohesin-dockerin

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interaction by rational mutagenesis. In: K. Ohmiya, K. Sakka, S. Karita, T. Kimura,

& M. Sakka (Eds) In: Genetics, Biotechnology of Lignocellulose Deg

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