EVA R. CHIN

Assistant Professor

School of Public Health

University of Maryland

College Park, MD 20740

Ph 301-***-****

Fax 301-***-****

Cell 860-***-****

e-mail: abqcp6@r.postjobfree.com

EDUCATION

1989-1993 University of Waterloo Ph.D.

Faculty of Applied Health Kinesiology

Sciences

1987-88 Queens University B.Ed.

Faculty of Education

1985-87 McMaster University M.Sc.

Adapted Human Biodynamics Exercise Physiology

Program

1981-85 University of Windsor B.H.K.

Faculty of Human Kinetics

SOCIETY MEMBERSHIPS

American Physiological Society

American College of Sports Medicine

OTHER PROFESSIONAL ACTIVITIES

Journal Review Panels

1999-2001 Editorial Review Board, Journal of Applied Physiology

1996-present Reviewer, Journal of Applied Physiology

1996-present Reviewer, Canadian Journal of Physiology and Pharmacology Reviewer, Plugers Archiv

1998-present Reviewer, Journal of Physiology

2001-present Reviewer, American Journal of Physiology

2005-present Reviewer, Molecular Biology of the Cell

2009-present Reviewer, PLoS One

Grant Review Committees Reviewer, Australian Research Council Grant Review Committee member (Integrated Animal Biology) for Natural Science Engineering & Research Council of Canada (NSERC)

2009-2011 Member, Vanier Canada Graduate Scholarship Selection Committee (NSERC)

2009-2011 Member, Movement and Exercise Committee, Canadian Institutes of Health Research (CIHR)

Thesis/Dissertation Examination Committees Thomas J. Hawke, University of Guelph

Ph.D. Dissertation: Intracellular calcium’s role in methylxanthine-induced changes in

potassium uptake in mammalian skeletal muscle

Oral and written thesis examination

Francois M. Viau, Laurentian University

M.Sc. Thesis: The role of Ca2+ and calcineurin in regulating the myofibrillar and

metabolic properties of individual skeletal muscle fibres

Written thesis examination

2005 Nicole Stupka, University of Melbourne

Ph.D. Dissertation: The calcineurin signal transduction pathway in muscular

dystrophy and skeletal muscle regeneration.

Written thesis examination

2007 Joseph Eibl, Laurentian University

M.Sc. Thesis: Deciphering the calcineurin/NFAT pathway in the hypertrophy and fiber type conversions of skeletal muscle

Oral and written thesis examination

2009 Ryan Sheppard, University of Maryland

Ph.D. Dissertation: Effects of androgen receptor polyglutamine repeat on C2C12 cell cycle and gene expression

Oral and written thesis examination

2010 Mallory Marshall, University of Maryland

M.A. Thesis: The effects of diet and physical activity on telomere length and telomere-related genes in mice bred for high voluntary wheel running

Oral and written thesis examination

RESEARCH EXPERIENCE

September 2008 – current University of Maryland, College Park: Assistant ProfessorEstablished new research lab focused on Molecular Basis of Muscle Function. Research lab currently focusing on single muscle fibre contractility and Ca2+ imaging. Model system being used to investigate mechanisms underlying muscle dysfunction with aging, muscular dystrophy and neurodegenerative diseases (i.e. ALS, SMA). Based on mechanisms of the disease we are evaluating treatments (exercise, diet and pharmacological strategies) that will attenuate these impairments.Research also focusing on basic cellular and molecular mechanisms regulating fast and slow muscle fibre-type specific gene expression using cell culture models. Tranlsational research wth Dr. Andrew Goldberg, MD, VA Medical Center Baltimore, investigating changes in human muscle glycoproteins in diabetic subjects and reversal of glycoprotein accumulation with exercise intervention.Evaluating muscle regenerative capacity using muscle-derived satellite cells and its application to: i) muscle tissue bioengineering for development of a small molecule screening platform and ii) muscle repair under disease or injury conditions.

April 2006 – August 2008 Pfizer Global Research & Development (Groton labs): Associate Director

Global Project Management: Cardiovascular & Metabolic Disease support Research Candidate Management Teams in planning, conducting and reviewing data from clinical trails for novel proprietary compounds in obesity, osteoporosis and frailty

April 2004 – April 2006 Pfizer Global Research & Development (La Jolla labs): Principal Scientist

Research Pharmacology (Diabetes & Obesity)Lead a small group of scientists conducting in vitro and in vivo pharmacological studies aimed at identifying small molecule and antibody-based therapies that are efficacious at lowering blood glucoseFocus on mechanisms for overcoming fatty acid-induced insulin resistance in muscle, adipose and liver through targeting MAPK signalling pathways and for increasing muscle mass by targeting myostatin signallingIn vitro assay development includes Cellomics nuclear translocation assays, Meso Scale Discovery phosphoprotein assays (include multi-spot arrays) and 3H 2-deoxyglucose uptake assays in myocytes and 3T3-L1 adipocytes In vivo studies include use of ob/ob, db/db and KKAy/a models of diabetes for efficacy and biomarker outcomes

December 1999 – April 2004 Pfizer Global Research & Development (Groton labs): Senior Research Scientist (Frailty Biology)Focused on identifying novel targets for drug discovery in new area of age-related muscle wasting (sarcopenia) Utilized micro-array and proteomics technologies, adenovirus and siRNA approaches as well as transgenic and knockout mouse models for target identification and validationDeveloped cell-based assays for moderate throughput screens of proprietary compounds including Cellomics-based nuclear translocation assaysDeveloped in vivo models for assessing motor performance to establish pre-clinical efficacy of clinical candidates; included use of genetically modified mice, ob/ob, and mdx miceEstablished flourescence microscopy technique for assessing myocyte intracellular Ca2+ levels in aged and atrophied muscleFocused on myostatin and IGF-1 signaling pathways Managed external grants to academics including Dr. Karyn Esser (University of Illinois at Chicago; now at University of Kentucky), Dr. George Ordway (University of Texas Southwestern Medical Center at Dallas), Dr. Richard Harvey (Victor Chang Cardiac Research Institute, Sydney Australia) and Professor David Allen (University of Sydney)

July-December 1999 University of Queensland: LecturerSet up research lab to investigate the molecular basis for skeletal muscle adaptation to exercise training.Established cell culture model for investigating Ca2+ -dependent pathways of gene expression using quantitative rt-pcrHeld three internal grants funded through the Australian Research Council (~ $60,000 per yr)

October 1996-June 1999 University of Texas Southwestern Medical Center: Research FellowInvestigated the molecular basis for fibre-type specificity of gene expression in skeletal muscleDevloped expertisein techniques of molecular biology which can be applied to understanding the molecular basis of muscle plasticity, including: DNA cloning, tissue culture work, gene transfection, generation of transgenic mice, genotyping using southern blot and PCR screening and gene expression analysis using RT-PCR, northern blot and western blot techniquesEstablished expertise in Ca2+ -dependent pathways of regulating muscle gene expression including use of cell-based and transgenic mouse models

August 1993-October 1996 University of Sydney, NH&MRC Research OfficerInvestigated the regulation of intracellular Ca2+ in isolated single fibres during muscle fatigueTrained in techniques of micro-dissection, micro-injection and the use of fluorescent dyes and caged compounds for single cell analysisDeveloped expertise in the role of intracellular Ca2+ in both acute and prolonged muscle fatigue

Sept. 1989-July 1993 University of Waterloo, Ph.D. CandidateModified and implemented several techniques for assessing skeletal muscle membrane function Co-ordinated research into sarcoplasmic reticulum Ca2+ regulation during muscle fatigue

September 1985- McMaster University, M.Sc. Candidate

September 1987 Supervisors: Dr. J.D. MacDougall and Dr. G.J.F. HeigenhauserDesign and completion of thesis research investigating ionic and metabolic regulation in inactive skeletal muscle

May 1983- University of Windsor, Summer Research Assistant

September 1983 Assistance with experiments investigating myosin ATPase plasticity during compensatory hypertrophy in rat skeletal muscle

TEACHING & SUPERVISORY EXPERIENCE

January 2009 – current University of Maryland, College ParkTeaching courses in Exercise Physiology cluster Undergraduate courses: KNES497 Senior Thesis Research, KNES497-0104 Adaptations of Skeletal Muscle with Disease and Exercise Training

and KNES498 Advanced Applied Physiology. Graduate course: KNES691 Muscular Aspects of Exercise Physiology

January - May 2007

& January-May 2008 Connecticut CollegeAdjunct Professor for Human Physiology undergraduate course.Course designed as a studio class with mini-lectures, lab activities, group discussion and presentation. Focus is problem-based learning.

December 1999 - present Pfizer Global Research & DevelopmentSupervise research associates involved in developing high throughput screening assays, cell-based signalling assays, analysis of tissues ex vivo for signalling proteins and gene expression and in vivo drug screeningTrained and supervised undergraduate students during summer research internships

July - December 1999 University of QueenslandLectured undergraduate and graduate students in exercise physiology courseCo-ordinated undergraduate and graduate level exercise physiology laboratory course

November 1997- UT Southwestern Medical Center

June 1999 Lead team project investigating the role of calcineurin in the regulation of gene expression and hypertrophic growth in skeletal muscleSupervised 2 full-time and 1 part-time technical staff and 1 graduate student

June-July 1998 UT Southwestern Medical CenterTraining and supervision of rotating medical student during summer research experience

August 1994 - University of Sydney

October 1995 Casual lecturer in graduate course in Applied Physiology - Exercise Sports Science/Sports Physiologylecturer for topics of metabolic regulation and muscle fatigue

December 1992- University of Waterloo

July 1993Lectured in undergraduate and graduate courses in exercise and muscle physiologyRepresentative of Graduate Student Association on committee set up by the University President to evaluate the use and needs of the students physical activity complex

PUBLICATIONS

Refereed Publications

Chin, E.R., M.I. Lindinger and G.J.F. Heigenhauser. (1991). Lactate metabolism in inactive skeletal muscle during lactacidosis. American Journal of Physiology 261 (Regulatory Integrative Comp. Physiol. 30): R98-R105.

Green, H.J., M. Ball-Burnett, E.R. Chin and D. Pette. (1993). Time dependent increases in Na+/K+ ATPase content of chronically stimulated rabbit muscle. FEBS, 310(2): 129-131).

Green, H.J., E.R. Chin, M. Ball-Burnett and D. Ranney. (1993). Increases in human skeletal muscle Na+-K+ ATPase pump concentration with short term training. American Journal of Physiology, 264 (Cell Physiol. 33): C1538-C1541.

Chin, E.R. and H.J. Green. (1993). Fibre type differences in Na+ -K+ ATPase concentration of rat skeletal muscle are related to oxidative potential. Canadian Journal of Physiology and Pharmacology, 71(8): 615-618.

Dossett-Mercer, J., H.J. Green, E. Chin and F. Grange. (1994). Preservation of sarcoplasmic reticulum Ca2+ sequestering function in homogenates of different fibre type composition following sprint activity. Canadian Journal of Physiology and Pharmacology, 72: 1231-1237.

Chin, E.R., H.J. Green, F. Grange, J.D. Mercer and P.J. O'Brien. (1994). Technical considerations for assessing alterations in sarcoplasmic reticulum Ca2+ sequestration function in vitro. Molecular and Cellular Biochemistry, 139: 41-52.

Dossett-Mercer, J., H.J. Green, E.R. Chin and F. Grange. (1995). Failure of short term stimulation to reduce sarcoplasmic reticulum Ca2+ ATPase function in homogenates of rat gastrocnemius. Molecular and Cellular Biochemistry, 146(1): 23-33.

Chin, E.R., H.J. Green, F. Grange, J. Dossett-Mercer and P.J. O'Brien. (1995). Effects of prolonged low frequency stimulation on skeletal muscle sarcoplasmic reticulum. Canadian Journal of Physiology and Pharmacology, 73: 1154-1164.

Chin, E.R. and H.J. Green. (1996). Effects of tissue fractionation on exercise-induced alterations in sarcoplasmic reticulum function in rat gastrocnemius muscle. Journal of Applied Physiology, 80(3): 940-948.

Chin, E.R. and D.G. Allen. (1996). The role of elevations in intracellular [Ca2+] in the development of low frequency fatigue in mouse single muscle fibres. Journal of Physiology, 491.3: 813-824.

Allen, D.G., C.D. Balnave and E.R. Chin (1996). Intracellular calcium release and muscle fatigue. [Review]. Today’s Life Sciences. Vol. 8 (9): 12-17.

Chin, E.R., M.I. Lindinger, and G.J.F. Heigenhauser. (1997). Distribution of lactate and other ions in inactive skeletal muscle: Influence of hyperkalemic lactacidosis. Canadian Journal of Physiology and Pharmacology, 75: 1375-1386.

Chin, E.R., C.D. Balnave and D.G. Allen. (1997). The role of intracellular calcium and metabolites in low frequency fatigue of mouse skeletal muscle. American Journal of Physiology. 272 (Cell Physiology 41): C550-C559.

Chin, E.R. and D.G. Allen. (1997). The effects of reduced muscle glycogen concentration on force, Ca2+ release and contractile protein function in intact mouse skeletal muscle. Journal of Physiology. 498: 587-600.

Chin, E.R. and D.G. Allen. (1998). The contributions of pH-dependent mechanisms to fatigue at different workloads in mammalian single muscle fibres. Journal of Physiology. 512: 831-840.

Chin, E.R., E.N. Olson, J.A. Richardson, Q. Yang, C. Humphries. J.M Shelton, H. Wu, W. Zhu, R. Bassel-Duby and R.S. Williams. (1998). A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type. Genes & Development 12: 2499-2509.

Garry, D.J., G.A. Ordway, J.N. Lorenz, N.B. Radford, E.R. Chin, R.W. Grange, R. Bassel-Duby and R.S. Williams. (1998). Mice without myoglobin. Nature. 395: 905-908.

Ordway, G.A., P. D. Neufer, E. R. Chin, and G. N. DeMartino. (2000). Chronic contractile activity upregulates the proteasome system in rabbit skeletal muscle. J. Appl. Physiol. 88: 1134-1141.

Wu, H., F.J. Naya, T.A. McKinsey, B. Mercer, J.M. Shelton, E.R. Chin, A.R. Simard, R.N. Michel, R. Bassel-Duby, E.N. Olson and R.S. Williams. (2000). MEF2 responds to multiple calcium-regulated signals in the control of skeletal muscle fiber type. Embo J. 19(9): 1-11.

Dunn, S.E., E.R. Chin & R.N. Michel. (2000). Matching of calcineurin activity to upstream effectors is critical for skeletal muscle fiber growth. J. Cell. Biol. 151(3): 1-10.

Grange, R.W., A. Meeson, E.R. Chin, K.S. Lau, J.T. Stull, J.M. Shelton, R.S. Williams & D.J. Garry. (2001). Functional and molecular adaptations in skeletal muscle of myoglobin-mutant mice. Am. J. Physiol. 281(5): C1487-C1494.

Chin, E.R., R.W. Grange, R.F. Viau, R.S. Bassel-Duby, R.N. Michel and R.S. Williams. (2003a). Alterations in slow-twitch muscle phenotype in transgenic mice overexpressing the Ca2+ buffering protein parvalbumin. J. Physiol. 547: 649-663.

Chakkalakal, J.V., M.A. Stocksley, M.A. Harrison, L.M. Angus, J. Deschenes-Furry, S. St.Pierre, L.A. Megheny, E.R. Chin, R.N. Michel and B.J. Jasmin. (2003). Expression of utrophin A mRNA correlates with the oxidative capacity of skeletal muscle fibre types and is regulated by calcineurin. PNAS 100: 7791-7796.

Chakkalakal, J.V., M.A. Harrison, E.R. Chin, R.N. Michel and B.J. Jasmin. (2004). Stimulation of calcineurin signalling attenuates the dystrophic pathology in mdx mice. Human Molecular Genetics 13(4): 379-388.

Chin, E.R. (2004). The role of Ca2+ and the Ca2+/calmodulin-dependent kinases in skeletal muscle plasticity and mitochondrial biogenesis. Proceedings of the Nutrition Society. 63: 279-286.

Michel, R.N., S.E. Dunn and E.R. Chin. (2004). Calcineurin and muscle growth. Proceedings of the Nutrition Society. 63: 341-349.

Hornberger, T.A., R. Stuppard, K.E. Conley, M. Fedele, M.L. Fiorotto, E.R. Chin and K.A. Esser. (2004). Mechanical stimuli regulate rapamycin-sensitive signalling by a phosphoinositide-3-kinase, protein kinase B and growth factor independent mechanism. Biochemical Journal. 380: 795-804.

Hornberger, T.A., R.D. Mateja, E.R. Chin, J.L. Andrews and K.A. Esser (2005). Aging does not alter the mechanosensitivity of the p38, p70S6k and JNK2 signaling pathways in skeletal muscle. J. Appl. Physiol. 98: 1562-1566.

Chin, E.R. (2005). The role of Ca2+/calmodulin-dependent kinases in skeletal muscle plasticity. J. Appl. Physiol. 99(2): 414-23.

Angus, L.M., J.V. Chakkalakal, A. Mejat, J.K. Eibl, G. Belanger, L.A. Megheny, E.R. Chin, L. Schaeffer, R.N. Michel and B.J. Jasmin. (2005). Calcineurin/NFAT signaling, together with GABP and PGC-1α, drives utrophin gene expression at the neuromuscular junction. Am. J. Physiol. Cell Physiol. 289: C908-C917.

Chakkalakal, J.V., S.A. Michel, E.R. Chin, R.N. Michel and B.J. Jasmin. (2006). Targeted inhibition of Ca+2/calmodulin signaling exacerbates the dystrophic phenotype in mdx mouse muscle. Hum. Mol. Gen. 15: 1423-1435.

Michel, R.N., E.R. Chin, J.V. Chakkalakal, J.K. Eibl and B.J. Jasmin. (2007). Ca2+/Calmodulin-based signalling in the regulation of the muscle fibre phenotype and its therapeutic potential via modulation of utrophin A and myostatin expression. Appl. Physiol. Nutr. Metab. 32: 921-929.

Cairns, S., E.R. Chin and J.M. Renaud. (2007). Stimulation pulse characteristics and electrode configuration determine site of excitation in isolated mammalian skeletal muscle: Implications for fatigue. J. Appl. Physiol. 103: 359-368.

Chin, E.R. (2010). Intracellular Ca2+ signaling in skeletal muscle: Decoding a complex message. Exerc. Sport Sci. Rev. 38(2): 76-85.

Sheppard, R.L., E.E. Spangenburg, E.R. Chin, S.M. Roth. (2011). Androgen receptor polyglutamine repeat length affects receptor activity and C2C12 cell development. Physiol. Gen. Published online before print August 2011, doi: 10. 1152.

Published Abstracts/Conference presentationGalea, V., E.R. Freisinger, M.I. Lindinger and G.F.J. Heigenhauser. (1987). Effects of lactacidosis on skeletal muscle ion regulation. Medicine and Science in Sports and Exercise. Suppl. 19(2): S35. Freisinger, E.R., M.I. Lindinger, V. Galea and G.J.F. Heigenhauser. (1987). Effects of lactacidosis on resting skeletal muscle metabolism in isolated perfused rat hindlimb. Medicine and Science in Sports and Exercise. Suppl. 19(2): S54.

Freisinger, E.R., M.I. Lindinger, V. Galea, J.D. MacDougall and G.J.F. Heigenhauser. (1987). Effects of arterial lactacidosis on fat and carbohydrate metabolism in inactive skeletal muscle. Canadian Journal of Sport Sciences. 12(3): 7P.

Chin, E.R., M.I. Lindinger and G.J.F. Heigenhauser. (1990). Effects of lactacidosis on ionic flux across inactive skeletal muscle. Canadian Journal of Sport Sciences. 15(4): 7S.

Chin, E.R. and H.J. Green. (1991). Fibre type differences in Na+ -K+ ATPase concentration of rat skeletal muscle are related to oxidative potential. Medicine and Science in Sports and Exercise. Suppl. 23(4): S23.

Chin, E.R., H.J. Green, M. Ball-Burnett and D. Ranney. (1992). Training induced changes in sarcolemmal Na+ -K+ pump concentration in human skeletal muscle. Medicine and Science in Sports and Exercise. Suppl. 24(5): S106.

Green, H.J., F. Grange, E.R. Chin, C. Goreham, D. Ranney and R.J. Xiu. (1992). Alterations in sarcoplasmic reticulum Ca2+ ATPase activity in human skeletal muscle with prolonged exercise. The Physiologist. 35(4): 215.

Chin, E.R., F. Grange, J.D. Mercer and P.J. O'Brien. (1992). Technical considerations for assessing skeletal muscle sarcoplasmic reticulum function following exercise. The Physiologist. 35(4): 215.

Chin, E.R., H.J. Green, F. Grange, J.D. Mercer, P.J. O'Brien. (1993). Functional impairment of sarcoplasmic reticulum Ca2+ -Mg2+ ATPase in vitro is related to altered membrane yield following prolonged running. The FASEB Journal. 7(3): A226.

Dossett-Mercer, H.J. Green, E.R. Chin, F. Grange and P.J. O'Brien. (1993). Lack of an effect of short term stimulation on sarcoplasmic reticulum Ca2+ uptake and Ca2+ ATPase activity in homogenates of rat red and white gastrocnemius. Medicine and Science in Sports and Exercise. Suppl. 25(5): S177.

Chin, E.R., H.J. Green, F. Grange, J.D. Mercer, P.J. O'Brien. (1993). Muscle fatigue in situ is independent of altered sarcoplasmic reticulum function in vitro following prolonged electrical stimulation. Medicine and Science in Sports and Exercise. Suppl. 25(5): S19

Chin, E.R. and H.J. Green. (1994). Skeletal muscle Ca2+ ATPase activity in heavy and light sarcoplasmic reticulum fractions following treadmill running. Proceedings of the Australian Physiological and Pharmacological Society. 25(1): 25P.

Chin, E.R., F. Grange and H.J. Green. (1994). Calcium uptake in heavy and light sarcoplasmic reticulum of skeletal muscle following treadmill running. Proceedings of the Australian Physiological and Pharmacological Society. 25(2): 114P.

Chin, E.R. and D.G. Allen. (1995). Effects of caffeine and tetanic stimulation on the force-frequency relationship in isolated single muscle fibres. Proceedings of the Australian Physiological and Pharmacological Society. 26(1): 2

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