Jeffrey M. Redwine, Ph.D.

Summary: I am a research scientist with experience in GLP toxicology preclinical study

management, experimental design, and I have worked eight years in the biotech industry. I have

solid experience with quantitative stereologic techniques, histologic techniques, and

imaging/microscopy applied to rodent models of neurologic disease. I have experience with

deconvolution, confocal, epifluorescence, and standard transmitted light microscopy. I value

building positive working relationships with others, maintaining a positive team environment,

actively contributing to the drug discovery process, and working closely with others in a multi-

discipline environment.

Contact Info: abp9lr@r.postjobfree.com; resume with access to publications: http://jeffredwine.com

EDUCATION

Ph.D. Neuroscience 1993 - 1998

Uniformed Services University of the Health Sciences (USUHS)

Bethesda, Maryland; Department of Neuroscience

Dissertation: The Potential Role of PDGF in Oligodendrocyte

Repopulation of Demyelinated Lesions in Mouse Spinal Cord

B.A. Biopsychology and Philosophy 1984 - 1990

California State University, Chico

PROFESSIONAL EXPERIENCE

Research Scientist 2008 - present

Charles River Laboratories

Serve as a research scientist and as a Study Director in the

conduct of assigned nonclinical research studies, to including study

management, interpretation and reporting of study data, and

assuring the regulatory compliance of these projects.

Senior Research Scientist 2006 - 2008

BrainCells Inc., San Diego, California

Supervised histology group that performed histology-based drug

screening by quantifying effects of candidate drug therapies for

effects on cell proliferation and neurogenesis in the rodent

hippocampus. Responsibilities included supervising team

members, data analysis and presentation, development of novel

assays, coordination of outside contract projects.

Senior Staff Scientist 2001 - 2006

Neurome Inc., La Jolla, California

Characterized rodent models of neurologic disease by employing

quantitative stereological techniques measuring cell number,

volume, or neurochemical changes; applied to Alzheimer s disease

J.M. Redwine

(Tg models), multiple sclerosis (EAE), Parkinson s Disease

(MPTP model), ALS (SOD1 Tg mice), neurogenesis.

Responsibilities included supervision of laboratory technicians,

Vivarium Manager, IACUC Chair, coordination of outside contract

projects.

Postdoctoral Fellow 1998 - 2001

Department of Neuropharmacology, The Scripps Research Institute,

La Jolla, California

Characterized CNS inflammatory response in mouse models of

virus-induced demyelination or inflammation by employing multi-

label immunofluorescence imaged with confocal microscopy to

identify cell types expressing MHC molecules within the CNS.

Collaborated with others using confocal microscopy localizing

viral proteins to organelles, and colocalizing viral proteins within

cultured cells.

Biological Laboratory Technician 1990 - 1993

National Institute of Child Health and Human Development, NIH,

Bethesda, Maryland

TECHNICAL SKILLS

Stereology Training Course: Applications of unbiased stereology to neural systems.

Stereology Resource Center (SRC), 2001, San Diego, CA

Stereology immunohistochemistry PCR

MRI volumetric analysis frozen tissue sectioning northern blot

high resolution fluorescence mammalian cell culture SDS-PAGE

imaging: deconvolution primary glial cultures DNA sequencing

microscopy, laser scanning in situ hybridization virus propagation

confocal microscopy cloning plaque assay

multi-label immunofluorescence transfection isoelectric focusing

strong

communication and presentation skills biohazard safety level-3 procedures

teachingassistant experience in neuroanatomy abilityto work independently or

computer data and image processing collaboratively

PUBLICATIONS

Peer-Reviewed Publications

1. Broide, R.S., Redwine, J.M., Aftahi, N., Young, W., Bloom, F.E., Winrow, C.J. (2007)

Distribution of histone deacetylases 1-11 in the rat brain. J. Mol. Neurosci. 31:47-58.

2. Jacobsen, J.S., Wu, C.-C., Redwine, J.M., Comery, T.A., Arias, R., Bowlby, M., Martone, R.,

Morrison, J.H., Pangalos, M., Reinhart, P., Bloom, F.E. (2006) Early-onset behavioral

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J.M. Redwine

and synaptic deficits in a mouse model of alzheimer s disease. Proc. Nat. Acad. Sci.

USA 103:5161-5166.

3. Hovatta, I., Tennant, R.S., Helton, R., Marr, R.A. Singer, O., Redwine, J.M., Schadt, E.E.,

Ellison, J.A., Schadt, E.E., Verma, I.M., Lochart, D.J., Barlow, C. (2005) Glyoxalase 1

and glutathione reductase regulate anxiety in inbred mouse strains. Nature 438:662-

666.

4. Redwine, J.M., Kosofsky, B.E., Jacobs, R.E., Games, D., Reilly, J.F., Morrison, J.H., Young,

W.G., Bloom, F.E. (2003) Dentate gyrus volume is reduced before onset of plaque

formation in PDAPP mice: a magnetic resonance microscopy and stereologic analysis.

Proc. Nat. Acad. Sci. USA 100:1381-1386.

5. Albrecht, P.J., Murtie, J.C., Ness, J.K., Redwine, J.M., Armstrong, R.A., Levison, S.W.

(2003) CNTF is upregulated in astrocytes during the remyelination phase of viral-induced

spinal cord demyelination and stimulates FGF-2 production in spinal cord astrocytes.

Neurobiol. Dis. 13:89-101.

6. Rodriguez, F., Harkins, S., Redwine, J.M., DE Pereda, J.M., Whitton, J.L. (2001) CD4+ T

cells induced by a DNA vaccine: immunological consequences of epitope-specific

lysosomal targeting. J. Virol. 75:104**-*****.

7. Patterson, J., Cornu, T., Redwine, J.M., Dales, S., Lewicki, H., Holz, A., Thomas, D.,

Billeter, M.A., Oldstone, M.B.A. (2001) Evidence that the hypermutated M protein of a

subacute sclerosing panencephalitis measles virus actively contributes to the chronic

progressive CNS disease. Virology 291:215-225.

8. Redwine, J.M., Buchmeier, M.J., Evans, C.F. (2001) In vivo expression of major

histocompatibility complex molecules on oligodendrocytes and neurons during viral

infection. Am. J. Path. 159:1219-1224.

9. Redwine, J.M., Armstong, R.C. (1998) In vivo proliferation of oligodendrocyte progenitors

expressing PDGF- receptor during early remyelination. J. Neurobiol. 37:413-428.

10. Lee, J.H., Novoradovskaya, N., Rundquist, B., Redwine, J., Saltini, C., Brantly, M. (1998)

An -1 antitrypsin nonsense mutation associated with a retained truncated protein and

reduced mRNA. Mol. Gen. Metab. 63:270-280.

11. Redwine, J.M., Blinder, K.L., Armstrong, R.C. (1997) In situ expression of fibroblast growth

factor receptors by oligodendrocyte progenitors and oligodendrocytes in adult mouse

CNS. J. Neurosci. Res. 50:229-237.

12. Krasnewich, D.M., Holt, G.D., Brantly, M.L., Skovby, F., Redwine, J., Gahl, W.A. (1995)

Evidence of abnormal synthesis of dolichol-linked oligosaccharides in carbohydrate-

deficient glycoprotein syndrome. Glycobiology 5:503-510.

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J.M. Redwine

13. Patchev, V.K., Mastorakos, G., Brady, L.S., Redwine, J., Chrousos, G.P. (1993) Increased

arginine vasopressin secretion may participate in the enhanced susceptibility of lewis rats

to inflammatory disease. Neuroendocrinology 58:106-110.

14. Karalis, K., Sano, H., Redwine, J., Wilder, R., Chrousos, G.P. (1991) Autocrine or paracrine

inflammatory actions of corticotropin releasing hormone in vivo. Science 254:421 423.

Reviews/Chapters

1. Bloom, F.E., Reilly, J.F., Redwine, J.M., Wu, C.C., Young, W.G., Morrison, J.H. (2005)

Mouse models of human neurodegenerative disorders: requirements for medication

development. Arch. Neurol. 62:185-187.

2. Bloom, F.E., Morrison, J.H., Redwine, J.M., Reilly, J.F., Young, W.G. (2004) Information

technology in drug discovery: neuroinformatics tools for visualizing gene expression in

the brain. Biosilico 2:112.

3. Armstrong, R.C., Redwine, J.M., Messersmith, D.J. (2005) Coronavirus-induced

demyelination and spontaneous remyelination: growth factor expression and function.

In: Experimental models of multiple sclerosis, Lavi, E. and Constantinescu, C.S. (Eds).

Springer, New York, 793-803.

4. Bloom, F.E., Morrison, J.H., Young, W.G., Redwine, J.M., Reilly, J.F. (2004) Mapping

pathways from genes to brain diseases with applied neuroinformatics. Human

Genomics.

5. Redwine, J.M., Evans, C.F. (2002) Markers of central nervous system glia and neurons in

vivo during normal and pathological conditions. Cur. Top. Microbiol. Immunol.

265:119-140.

6. Evans, C.F., Redwine, J.M., Patterson, C.E., Askovic, S., Rall, G.F. (2002) LCMV and the

central nervous system: uncovering basic principles of CNS physiology and virus-

induced disease. Cur. Top. Microbiol. Immunol. 263:177-196.

FELLOWSHIPS AND AWARDS

Advanced Postdoctoral Fellowship, The National Multiple Sclerosis Society (FA 1407-A-1),

2000-2003- $113,184 (returned due to job change)

NIH Training Grant, Dept. of Neuropharmacology, The Scripps Research Institute; Michael

B.A. Oldstone, T32 (A600080-19), 1998-2000

Travel Award, Society of Fellows Spring Research Symposium, The Scripps Research Institute,

1999- $800

Student Travel Award, American Society for Neurochemistry/ International Society for

Neurochemistry, Boston MA, 1997- $700

Sustained Superior Performance Award, Henry M. Jackson Foundation for the

Advancement of Military Medicine, 1997- $450

Best Oral Presentation, USUHS 16th Annual Graduate Research Colloquium, 1996

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J.M. Redwine

INVITED TALKS

Redwine, J.M., Shriver, L.P., Oldstone, M.B.A., Buchmeier, M.J., Evans, C.F. (2000) In situ

expression of MHC class I on oligodendrocytes during virus-induced inflammation and

demyelination. American Society for Neurochemistry, Chicago, IL

SELECTED PUBLISHED ABSTRACTS

Redwine, J.M., Arthurs, S., Games, D., Jacobs, R.E., Young, W.G., Morrison, J.H., Kosofsky,

B.E., Bloom, F.E. (2002) Specific volumetric reductions precede plaque formation in

PDAPP mouse model of Alzheimer s Disease by magnetic resonance microscopy. Society

for Neuroscience, Orlando, FL.

Redwine, J.M., Shriver, L.P., Oldstone, M.B.A., Evans, C.F. (1999) B7-1 expression by

oligodendrocytes enhances viral-induced CNS autoimmune disease. J. Neurochemistry

72:S70B.

Redwine, J.M., Blinder, K.L., Armstrong, R.C. (1997) Oligodendrocyte lineage cells express

PDGF R and FGFR in remyelinating mouse spinal cord. J. Neurochemistry 69:S26D.

Redwine, J,M., Armstrong, R.C. (1996) Expression of platelet-derived growth factor -receptor

and ligand during CNS remyelination. Society for Neuroscience 22(1):50, Washington,

D.C.

Redwine, J.M., Armstrong, R.C. (1996) Expression of platelet-derived growth factor receptor

and ligand in remyelinating mouse spinal cord. Gordon Conference on Myelin, Barga,

Italy.

Mahaney, K., Brantly, M., Redwine, J., Hoofnagle, J.H., Di Bisceglie, A.M. (1993) Alpha 1

antitrypsin levels and phenotypes and progression of liver disease in chronic viral hepatitis.

Hepatology 22 (Suppl).

Brantly, M., Laubach, V., Hildesheim, J., Leifer, J., Redwine, J., Mittereder, N., Trapnell, B.

(1993) Intron sequence dependent enhancement of 1-antitrypsin gene expression. Cold

Spring Harbor Laboratory, session on regulation of liver gene expression in health and

disease, p. 21.

Karalis, K., Sano, H., Redwine, J., Wilder, R., Chrousos, G.P. (1991) A novel role for

corticotropin releasing hormone: local secretion and auto/paracrine proinflammatory action

in vivo. Endocrine Society.

Kamilaris, T.C., Redwine, J., Smith, M.A., Johnson, E.O., Gold, P.W., Chrousos, G.P. (1990)

Effect of short and long duration hypothyroidism and hyperthyroidism on hypothalamic

corticotropin-releasing-hormone mRNA responses to stress. Society for Neuroscience

16:91, St. Louis, MO.

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