Igor Ernesto Bruzual Acevedo, PhD

**** ** ******* ** #** Portland Oregon 97219

Email: aboewc@r.postjobfree.com

Phone: 617-***-****

Academic Background

**** *** ********* ******* (2002)

Central University of Venezuela (Universidad Central de Venezuela).

Thesis: “Analysis of Heavy Metals Determinants

on plasmids of Inc HI3and HII”

Master in Cellular Biology (1996), Central University of Venezuela

1996

Research Experience:

Research Associate, Wong Laboratory, Infectious Disease Division,

2010 – present Oregon Health Science University, Portland OR. July 2010.

• Conduct original research on the mechanism of drug resistance in the

emerging fungal pathogen Cryptoccocus gatti., including:

• Molecular characterization of putative ATP’s drug pumps.

• Construction of the knockout mutants for 3 ATP’s pumps.

• Understanding of the mechanism of Heteroresistance in C. gatti.

• Oversee all aspects of laboratory management, including at the project,

personnel and regulatory levels (e.g. IACUC, IRB, IBC, and laboratory

and biosafety)

Research Associate, Kumamoto Laboratory, Tufts University, Boston,

2003-2008

MA .

• Effect of antifungal uses in different Candida albicans growth

conditions.

• Sequencing and analysis of the clinical isolated C. albicans MDR1,

CDR1 promoter region’s

• Expression analysis of the MDR1 promoter region.

• RFLP studies of the clinical isolated C. albicans strains.

• Dissection of C. albicans biofilm formation process.

• Starting the studies of biofilm formation in C. Kumamoto

Laboratory

• Measurements of metabolic activities on C. albicans biofilm

formation

• Expression analysis on C. albicans biofilm formation

• Project level management and review of research staff, including

laboratory manager and research assistants.

2002 –2003

• Mentored college students on summer programs and students enroll on

other programs.

Igor Bruzual Ph.D.

2386 SW Vermont St. #29 Phone: 617-***-****

Portland Oregon 97219 Email:aboewc@r.postjobfree.com

2001-2002

Professional researcher, Laboratorio de Biología de Plásmidos (Plasmid

1997-2002 Biology Lab.) (IBE) (Experimental Biology Institute), Dr. Guillermina Alonso,.

Heavy Metal resistance in H plasmids.

Internship, Martin Luther University, Halle Germany, Dr. Dietrich Nies Group..

1996-1997

Heavy Metal resistance in H plasmids.

Graduate Student, Plasmid Biology Laboratory, IBE, Mentor Dr. Guillermina

Alonso. Heavy Metal resistance in H plasmids.

Research Technician. Consejo de Desarrollo Científico y Humanístico (CDCH).

Universidad Central de Venezuela. Prof. Raquel Pedroza. November 96 through

March 97.

Publications

Bruzual, I and C. Kumamoto (2011) Homozygosis for an MDR1 promoter

allele with higher promoter activity is common in clinically isolated strains of

Candida albicans. Mol Genet Genomics. 2011 Dec;286(5-6):347-57]

Bruzual, I; Riggle, P.; Hadley, S. and. Kumamoto, C.A. (2007), Biofilm

Formation by Fluconazole-Resistant Strains is Inhibited by Fluconazole, J

Antimicrob Chemother. Mar;59(3):441-50

Alonso, G; Vílchez, G.; Bruzual, I. and Rodríguez Lemoine (2001)

Characterization of plasmid MIP233 (IncHI3) of the H Complex. Research

Microbiology 153:149-153

Alonso, G.; Campos, Jenny; Bruzual A., Igor E. y Rodríquez-Lemoine, V.

(2000) Constrution of a cassette for cloning and analysis of replicons. Acta

Científica de Venezolana 51 (1)

Alonso, G.; Bruzual A., Igor E.; Campos, Jenny y Rodríquez-Lemoine,

V. (1999) Cloning and characterization of a replicon region the IncHII

plasmid pHH1457. FEMS Microbilogy Letters 179: 361 – 366.

Manuscripts in preparation

Basso. L., Gast C., Bruzual, I and Wong B. Characterization of novel

transmembrane proteins pumps involved on drug resistance on the emerging

fungal pathogen Cryptoccocus gatti. To be submitted to Plos Pathogens

Gast C., Basso. L., Bruzual, I and Wong B Azole resistance in Cryptococcus

gattii strains from the Pacific Northwest: lack of evidence for a role of

ERG11. To be submitted to J Antimicrob Chemother.

Bruzual, I and C. Kumamoto C. albicans MDR1 gene expression profile in

Bioflim forming conditions. In preparation for submission to Journal of

Antimicrobial and Chemotherapy

Igor Bruzual Ph.D.

2386 SW Vermont St. #29 Phone: 617-***-****

Portland Oregon 97219 Email:aboewc@r.postjobfree.com

Teaching Experience

Adjunct Professor General Molecular Genetics, Graduate program in

2001 Medical Science, School of Pharmacy. June 2001- August 2001

Adjunct Professor. General Molecular Genetics, Graduate program in

Pharmacology, School of Pharmacy. April 2001-June 2001

2001

Adjunct Professor, General Genetics Lab. School of Science, Central

University of Venezuela. October 1998-March 1999

Meetings and Congress

1998

Bruzual A., Igor E., Perry Riggle and Carol A. Kumamoto. Regulation of

Candida albicans MDR1 Gene Expression under Biofilm Conditions 108th

ASM General Meeting Boston, MA, June 1 -5, 2008.

2008 Igor Bruzual. "Candida albicans MDR1 drug resistance: The chicken or

the egg". Boston Yeast Area Meeting, BAYM., Harvard FAS Center for

Systems Biology Cambridge MA 02138. March 17th 2008

2008 Igor Bruzual. “Biofilm Formation in Candida albicans: Fluconazole

resistance is futile”. Boston Yeast Area Meeting, BAYM., White Head

Institute MIT. November 15th 2006

200 Bruzual A., Igor E., Perry Riggle, Susan Hadley and Carol A.

6 Kumamoto. Biofilm Formation by Fluconazole-Resistant Strains is

inhibited by Fluconazole 9th ASM Conference on Candida and

Candidiasis, Denver, Colorado, March 13 -17, 2006

2006

Bruzual A., Igor E., Susan Hadley and Carol A. Kumamoto Reversible

Activation Of The MDR1 Promoter In Wild Type Cells Following

Exposure To Fluconazole 8thASM Conference on Candida and

Candidiasis, Austin, Texas, March 18 -22, 2004

2004 Bruzual A., Igor E.; Alonso, G. y Rodríquez-Lemoine,V Caracterización

Preeliminar de la zona de replicación del Plásmido pHH1457 (IncHII)

(Characterization of the replication zone of pHH1457 plasmid)XIII.

Congreso de Bioquímica Clínica. March, 1997. Macuto Sheraton Hotel,

Macuto, Venezuela

199

7

Bruzual A., Igor E.; Alonso, G. y Rodríquez-Lemoine, V. Clonamiento

de los replicones de plásmidos del HI (Subgrupo HI3) e IncHII (Replicon

Cloning of Inc HI (subgoup HI3) and HII plasmid). XLV Convención

Anual de la AsoVAC. November, 1995. Universidad Simón Bolívar.

Caracas, Venezuela.

1995

Igor Bruzual Ph.D.

2386 SW Vermont St. #29 Phone: 617-***-****

Portland Oregon 97219 Email:aboewc@r.postjobfree.com

Courses

Microbiology diversity and biotechnology possibility in the rain forest.

200

Unión de Universidades Amazónicas, Universidad Central de Ve nezuela,

2

Amazonia Network y Comunidad Europea, November – December 2002

Bacterial Genetics, Theoretical and Practical Course, ICGEB Trieste Italy, 8-13

May, 2000

200

Genetics Patterns use in Forensic, Ethical and Moral Implications . (Uso de

0

Perfiles Genéticos en Forénsica y Paternidad, Implicaciones Éticas y Morales),

ONU- Biolac-Idea. Caracas, Venezuela. February 1-10, 2000.

200

0

Awards

Scholarship granted by Consejo Nacional de Investigaciones Científicas y

2001-2002

Tecnológicas (CONICIT) for short time research in Matin Luther University,

Halle Germany, December -april 2002

Scholarship granted by Deutcher Akademischer Austauschdienst (DAAD),

2001

German Government, for short time research in the Matin Luther University,

Halle Germany, July- December 2001

Scholarship granted by Consejo Nacional de Investigaciones Científicas

1997-2002 (Nationjal Council of National Research, CONICIT to complete the graduate

school

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