YOU-JUN FU, Ph.D.

**** ********* ***, *********, ** 40513 ( 859-***-**** (

ablvf1@r.postjobfree.com

OBJECTIVE

A position of Scientist in Analytical Chemistry

SUMMARY OF QUALIFICATIONS

. Experience in hand-on operation, maintenance, and troubleshooting of

various HPLC systems and mass spectrometers

. Skills in LC/MS and GC/MS to quantify biogenic small molecules,

peptides, and proteins using stable isotope labeling

. Experience in application of mass spectrometry and NMR in structural

characterization of organic compounds

. Familiarity with Karl Fisher titration, FTIR, UV/Vis, DTA/TGA/DSC,

fluorescence spectrometry, electrochemical analysis, X-ray powder

diffraction

. Coding ability in BASIC, FORTRAN, C++ and data analysis ability in

Excel, statistical analysis programming and familiarity with

Microsoft Office

. Preparing manuscripts, technical progress, summary reports,

documentation, and making presentations

. Experienced in working in multifunctional, multi-task team

environment with strong problem solving ability

WORK EXPERIENCE

Scientist, Proteomics Core Facility/Department of Biochemistry, University

of Kentucky, Lexington, KY, Aug. 2009 - Present

Maintaining a mass spectrometry lab and using HPLC/ESI-MS to quantify

peptides in protein digests from cell culture and animal models.

. Improved sampling method in HPLC system to inject more samples in the

LC/MS experiments to enhance the detection sensitivity.

. Developed protocols combining Famos LC autosampler, Eksigent Nano LC

system, and Applied Biosystems Qstar XL mass spectrometer with

biological techniques, such as, immunoprecipitation, Western blot, to

study the protein alterations caused by ALS relevant mutations in

cell culture.

Postdoctoral scholar, UK Mass Spectrometry Facility/Department of

Chemistry, University of Kentucky, Lexington, KY, Oct. 2006

- Aug. 2009

. Leading and supervising graduate students in the collaborative

project (with Sanders Brown Aging Center, University of Kentucky) to

quantify brain mitochondrial changes in Alzheimer's disease patients

and mouse models using stable isotope labeling (ICAT) with LC

Packings micro/nano LC system and Thermo LCQ deca mass spectrometer.

(First author paper published)

. Collaborating with Department of Food and Animal Science, University

of Kentucky, to quantify membrane protein changes in Clostridium

thermocellum with alcohol treatment (First author paper in

preparation, poster presentation at ASMS conference)

. Developed a novel GC/MS method to quantify biogenic unsaturated

aldehydes using isotopic dilution using Saturn 3400 GC/MS/MS system

(Varian).

Research Assistant Professor, Department of Pharmaceutical Sciences,

Hampton University, Hampton, VA, Mar. 2006 - Sept. 2006

. Developed a HPLC method using UV and mass spectrometric detector for

in vitro identification of metabolites of MDMA incubated with human

liver hepatocytes.

Postdoctoral Research Associate, Washington State University/Pacific

Northwest National Laboratory, Richland, WA, Mar. 2002 -

Mar. 2006

. Developed a MS/MS method to study fragmentation properties of Fe-S

proteins and analogs using both in source CID and in cell CID

techniques (Home built 6 T FTMS).

Research Fellow, Nanyang Technological University, Singapore, Nov. 1999 -

Mar. 2002

. Synthesized a series of organic and inorganic luminescent materials

which were characterized by fluorescence, FTIR, UV/Vis absorption

spectroscopy, X-ray diffraction, and thermal analysis (DSC and TGA).

Associate Professor, State Key Laboratory of Crystal Materials, Shandong

University, Jinan, Shandong, P. R. China. Aug. 1996 - Nov.

1999

. Investigated the phase transition of bulk crystal materials by UV/Vis

absorption spectrometry and thermal analysis (TGA and DSC).

Postdoctoral Fellow, State Key Laboratory of Coordination Chemistry,

Nanjing University, Nanjing, Jiangsu, P. R. China, July 1994

- Aug. 1996

. Design, synthesis &structure characterization of novel supramolecular

structures, such as, helical structures of oligopyridine and

oligobipyridine derivatives under the direction of transition metals.

FINAL EDUCATION

Ph.D., Physical/Analytical Chemistry, Ocean University of China, P. R.

China, 1994

PROFESSIONAL SOCIETY MEMBERSHIP AND SERVICE

. American society for Mass Spectrometry (ASMS)

. American Chemical Society (ACS)

. Paper reviewer, Journal of the American Chemical Society (JACS), 2005

- 2006

RECENT PUBLICATIONS (More than 60 journal papers and 10 conference

presentations available)

1. Fu, Y.J.; Nelson, W., Lynn, B. C., Strobel, H. C. "Ethanol Tolerance of

Clostridium thermocellum: A Differential Quantitative Proteomic Analysis

of Cellular Fractions Using 15N-Metabolic Labeling Strategy", Journal of

Bacteriology, (in preparation).

2. Fu, Y.J.; Xiong, S., Lovell, M., Lynn, B.C. "Quantitative Proteomic

Analysis of Mitochondria in Aging PS-1 Transgenic Mice", Cellular and

Molecular Neurobiology, 2009, 29: 649-664.

3. Fu, Y.J.; Laskin, J., Wang, L.S. "Electronic Structure and Fragmentation

Properties of [Fe4S4(SEt)4-x(SSEt)x]2-, International Journal of Mass

Spectrometry, 263 (2007), 260-266.

4. Fu, Y.J.; Laskin, J., Wang, L.S. "Collision induced fragmentation of

[4Fe-4S] cubane cluster complexes: [Fe4S4Cl4-x(SC2H5)x]2- (x = 0 - 4)",

International Journal of Mass Spectrometry, 255-256 (2006), 102-110.

Contact this candidate