GAURAV KAUSHIK, Ph.D.

Phone: 208-***-****

Email: ablbkq@r.postjobfree.com

SUMMARY

Two years of industrial experience in upstream process development of biosimilars, monoclonal antibodies and vaccines.

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Eight years of mammalian cell culture experience (industrial and academic).

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Ph.D. in investigating the role of psychoactive pharmaceuticals in causing Autism Spectrum Disorders (ASD).

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Core competencies: Analytical and communication skills, motivational, hardworking and results-driven researcher.

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EDUCATION

Ph.D. Biology, Idaho State University, Pocatello, ID (GPA 3.91) 2015

M.S. Biotechnology, University College Cork, Cork, Ireland 2008

B.S. Biotechnology, Mahrishi Dayanand University, Haryana, India 2007

INDUSTRIAL EXPERIENCE

Panacea Biotec Limited, New Delhi, India Jun 2009 – Jul 2010

Research Associate, Upstream Process Development

Biosimilars & Monoclonal antibodies:

Successfully developed and optimized the yield of biosimilars (Enbrel, Darbepoetin) and Omalizumab products.

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Certified in bioreactor operation (Sartorius Biostat C plus and wave bioreactor) and its trouble-shooting.

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Improved the yield of Omalizumab from 56mg/l to 191mg/l in 5L Applikon stirred bioreactor.

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Optimized and improved the cell count of Omalizumab clone from 1million/ml to 3.3million/ml with 90% cell viability.

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Performed CHO cell line stability testing, media optimization, process optimization and yield optimization of proteins, and

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their validation studies in mammalian cell systems from spinner stage to bioreactor (small scale 1L-10L working volume).

Prepared documentations such as developmental and summary reports, and SOPs according to cGMP.

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Viral Vaccines:

Successfully developed and optimized yield (107 PFU/mL) of dengue vaccine in wave bioreactor.

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Established culture kinetics of mammalian vero cells on micro-carriers for optimal cell growth in the bioreactor.

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Optimized virus infection process at 0.3MOI for higher yield.

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Completed documentation of all working steps according to cGMP.

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Executive, Regulatory Affairs Oct 2008 – Jun 2009

Successfully prepared registration dossiers of vaccines and biopharmaceuticals for ROW countries like Chile, Kazakhstan,

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Jordan, Sri Lanka and Nepal.

Monitored R&D activities of “13 Valent Pneumococcal polysaccharide conjugate vaccine” project.

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Accomplished regulatory compliance of “13 Valent Pneumococcal polysaccharide conjugate vaccine” project.

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Reviewed documents like analytical method validation report, stability study report, various specifications, clinical trial

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report, periodic safety updating report and bioavailability studies report.

PUBLICATIONS

Cope G, Kaushik G, O’Sullivan SM, and Healy V: Gamma-melanocyte stimulating hormone regulates the expression and

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cellular localization of epithelial sodium channel in inner medullary collecting duct cells. Peptides 2013, 47(0): 54-59.

Kaushik G, Thomas MA, and Aho K. Psychoactive pharmaceuticals as environmental contaminants disrupt highly inter-

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connected nodes in an Autism-associated protein-protein interaction network. (In Press BMC Bioinformatics)

In process:

Kaushik G, Xia Y, Pfau JC, Cretekos C, Aho K, and Thomas MA. Dysregulation of Autism-associated synaptic proteins by

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environmental contaminants. (In Review Journal of Toxicology and Environmental Health)

Kaushik G, Xia Y, Yang L, Thomas MA. Psychoactive pharmaceuticals at environmental concentrations induce in vitro

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gene expression associated with neurological disorders. (Submitted BMC Genomics)

Kaushik G, Huber D, Aho K, Cretekos C, Bearden S, Finney B, and Thomas MA. In vivo intestinal and placental transfer of

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carbamazepine at very low concentrations from drinking water to the developing fetus brain. (Ready to submit PLOS ONE)

Updated: January 20, 2015

CONFERENCES ATTENDED

Oral Presentations

Kaushik G, Thomas MA, and Aho K. Psychoactive pharmaceuticals as environmental contaminants disrupt highly inter-

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connected nodes in an Autism-associated protein-protein interaction network. Biotechnology and Bioinformatics Symposium.

(Provo, UT 2014)

Kaushik G, Aho K, and Thomas MA. Network analysis of genes associated with Autism Spectrum Disorders (ASD),

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perturbed in fish brains by environmental contaminants. Idaho INBRE Summer Research Conference. (Moscow, ID 2013)

Kaushik G, Aho K, and Thomas MA. Altered gene expression in Pimephales promelas fish brains exposed to psychoactive

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pharmaceuticals is associated with Autism Spectrum Disorders in protein-protein interaction network. AAAS Pacific Division

Conference. (Las Vegas, NV 2013)

Poster Presentations

Kaushik G, Pfau JC, Cretekos C, Aho K, and Thomas MA. Dysregulation of Autism-associated synaptic proteins by

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psychoactive pharmaceuticals at environmental concentrations. Society of Toxicology Annual Meeting. (Phoenix, AZ 2014)

Kaushik G, Aho K, and Thomas MA. Altered gene expression in Pimephales promelas fish brains exposed to psychoactive

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pharmaceuticals is associated with Autism Spectrum Disorders in protein-protein interaction network. iBEST- Inland

Northwest Genomics Research Syposium. (Moscow, ID 2013) Best Poster Award

Kaushik G, Aho K, and Thomas MA. Altered gene expression in Pimephales promelas fish brains exposed to psychoactive

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pharmaceuticals is associated with Autism Spectrum Disorders in protein-protein interaction network. Society of Toxicology

Annual Meeting. (San Antonio, TX 2013)

LABORATORY SKILLS

Cell culture (primary and immortal cell line), flow cytometry, microscopy, immunoblot, mouse colony maintenance,

pregnant mouse dissection, ELISA, PCR.

ACADEMIC EXPERIENCE

Idaho State University, Pocatello, Idaho (Research Fellow) Aug 2010 - Jan 2015

Thesis: The potential association of psychoactive pharmaceuticals at low concentrations with Autism Spectrum

Disorders (ASD).

Designed protein network using Cytoscape tool Cultured human neuronal cells and differentiated them.

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Analyzed Autism- associated synaptic protein Analyzed complete transcriptome through RNA-

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expression through Flow Cytometry. sequencing.

Carried out mice experiments & traced isotope-labeled

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pharmaceuticals in fetus brains at E10 stage.

University College Cork, Cork, Ireland (Research Assistant) 2007-2008

Thesis: Investigations into the molecular mechanisms underlying the natriuretic effects of gamma-melanocyte

stimulating hormone in the kidney.

Conducted in vitro experiments by culturing primary kidneys collecting duct cells of mice and analyzed the expression of

ENaC/Aquaporin channels using western-blot technique.

AWARDS/FELLOWSHIP

Doctoral of Arts Fellowship, Idaho State University 2013-2014

Travel Award for Society of Toxicology Conference 2014, Idaho State University 2014

Best Poster Award, iBEST- Inland Northwest Genomics Research Symposium 2013

TEACHING EXPERIENCE

Supervised Teaching Internships, Idaho State University

Advanced Topics in Bioinformatics (Graduate) Jan.-May 2014

Genome Evolution Seminar (Graduate) Aug.-Dec. 2013

Graduate Teaching Assistant, Idaho State University

Human Anatomy and Physiology I and II Lab 2010 – 2013

May – Jun 2013

General Biology Lab

PROFESSIONAL SERVICE AND COMMUNITY OUTREACH

Association of Indian Students, Idaho State University (President) 2013-2014

Biology Graduate Student Association (Treasurer) 2013-2014

Snake River Association for Neuroscience (Member) 2013-2014

Updated: January 20, 2015

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