Prabesh Gautam

***** ********* ****** ****., *******, Texas, 77093, apt#801

Phone: 580-***-****

E-mail: abl6sy@r.postjobfree.com

Objective

To obtain a position in a research laboratory where I can be a part of a team making a contribution to improving human health and welfare

Highlights of Qualifications

I am a self-motivated, experienced and enthusiastic biological scientist with a background in a variety of Lab techniques. I have extensive experience with lab equipment, histopathological & cytological equipment and techniques, cell culture and molecular biology techniques, including PCR, RT PCR, ChIP assay, PLA, EMSA, western blot, DNA extraction and purification, gel electrophoresis, microscopy, serial dilution, and maintenance of bacterial, cell lines, and worm cultures. I have strong organizational and communication skills (both written and oral), as well as computational and bioinformatics skills for conducting projects involving DNA sequencing, gene annotation, sequence alignments and gene and exon identification.

EDUCATION

Bachelor of Science in Molecular Biology, GPA 3.23/4.00, May 2014

Minor in chemistry

East Central University, OK

Research & individual study

Project: Identification of melatonin resist mutant in C. elegans, East Central University

• Prepared different media, including NGM for worm culture

• Utilized molecular techniques such as PCR, DNA extraction and purification

• Handled standard biological equipment such as hemacytometers, thermocyclers, spectrometers, laminar flow hoods, UV light box and crosslinker

• Assisted lab mentor in developing innovative protocols and assays and logged technical data assessments and interpretations

• Developed a reliable melatonin sensitivity assay on C. elegans

• Identified GPCR mutants that are insensitive to melatonin

• Analyzed protein sequences of potential receptor mutants

• Isolated and cultured embryonic SNG::GFP cells

• Prepared the final research poster

Project: Cloning C. elegans GPCR genes (for RNA interference studies), East Central University

• Primer design and PCR

• Recombinant DNA techniques for gene cloning

• Bacterial transformation

• C. elegans culture and behavior analysis

• dsRNA expression and feeding

COURSEWORK

Cell and molecular biology, Biochemistry, microbiology, Molecular Genetics, Biotechnology, Molecular genetics, Advance molecular biology, Genomics and bioinformatics

TECHNICAL SKILLS AND LANGUAGE

•Efficiently conduct independent or group laboratory research and accurately record data

• Ability to thoroughly research literature and identify pertinent publications using the Internet

•Proficient in Microsoft Word, Excel, PowerPoint

•Handling computer files and Web

•Perform various computer programs (NCBI, Expasy Tools, UniProt, ProtParam, TopPred)

•Excellent verbal and written communication.

•Fluent in English, Nepali and Urdu

Volunteer Experience

• International Student Connection Club, member (2011-2014)

• Asian student Association member(2011-2014)

• Organized and planned events to host numerous events on campus for both international and

Domestic students to have a better understanding of diverse culture

• Organized fund raising events

• Assisted the international students with the transition to U.S. culture and society

• Organized blood donation program in the collaboration with Nepal Red Cross Society(department of microbiology, NITS, Nepal)

• Organized health education program to aware local people about different diseases by exhibition, newspaper, pamphlets, magazines(Nepal)

References available upon request

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