Research Assistant

David L. Evers

**** ********** **** *** #***

Gaithersburg, MD 20879

Phone: 240-***-****

e-mail: abiixa@r.postjobfree.com

Education

. Regulatory Compliance Certificate, in progress. Hood College,

Frederick, MD.

. Ph.D., Medicinal Chemistry, 2002. University of Michigan, Ann Arbor,

MI.

. B.S., Chemistry, 1996. University of Minnesota, Minneapolis, MN.

Expertise

. Biochemistry research, including molecular biology, assay development,

bioanalytical chemistry, tissue specimens/culture, and protein &

nucleic acid analysis.

Experience

. Research Associate, 2/07-now, Armed Forces Institute of Pathology,

Washington, DC. Supervisors: Drs. Jeffrey T. Mason & Timothy J.

O'Leary. Examined the effects of formalin-fixation upon RNA. Developed

assays for ultra-sensitive immunologic detection of proteins and

transcripts using liposome-based quantitative PCR reporters.

Approaches included nucleic acid isolation & hybridization, HPLC,

bioanalyzer electrophoresis, protease assays, bioconjugate strategies,

and histology. Trained junior staff; analyzed data, wrote reports,

funding proposals, protocols, and publications.

. Postdoctoral, 6/05-2/07, National Institute of Allergy and Infectious

Diseases, NIH, Bethesda, MD. Mentor: Dr. Jeffery K. Taubenberger.

Trained in negative stranded RNA virus molecular biology and host

tropism. Approaches included in vitro polymerase reporter assays,

cloning and expression, transient transfection, reverse genetics,

Sanger sequencing, site-directed mutagenesis, real-time PCR,

radioactive PCR, phylogenetic analyses, and hemagglutination assays.

Kept up on current scientific literature, trained junior staff, wrote

publications, and gave oral research presentations.

. Postdoctoral, 10/02-6/05, University of North Carolina, Chapel Hill,

NC. Mentor: Dr. Eng-Shang Huang. Proposed, researched, analyzed, and

reported the effects of antiviral drugs on cellular stress and signal

transduction pathways. Wrote funding proposals and publications; and

delivered lectures.

. Graduate Student, 9/96-10/02, University of Michigan. Advisors: Dr.

John C. Drach & Dr. Kevin G. Rice. Researched, analyzed, and reported

carbohydrate chromatography and the in vitro mechanisms of action for

antiviral drugs. Wrote funding proposals, protocols, and publications;

trained junior staff; and delivered lectures.

. Intern, 1996, Pfizer Global Research, Ann Arbor, MI. Combinatorial

chemistry.

. Undergraduate Student, 1995-1996, University of Minnesota, Synthetic

organic chemistry.

Teaching Experience

. Lecturer, 9/07-6/08, Department of Chemistry, Montgomery College,

Rockville, MD.

. Teaching Assistant, 1999 & 2002, Analytical chemistry and

biochemistry, University of Michigan, Ann Arbor, MI.

Awards

. American Chemical Society (Medicinal Chemistry) pre-doctoral

fellowship, 2000-2001.

. American Foundation for Pharmaceutical Education pre-doctoral

fellowship, 1999-2002.

Publications (several additional publications are in press or in

preparation)

17. O'Leary TJ, Fowler CB, Evers DL, Mason JT. 2009. Protein fixation and

antigen retrieval: chemical studies. Biotechnic & Histochemistry, 84:217-

221.

16. Evers DL, Slemons RD, Taubenberger JK. 2007. Impact of preservative

upon recoverable RT-PCR amplicon length from influenza A virus in bird

feces. Avian Diseases, 51:965-968.

15. Evers DL, Fowler CB, Cunningham RE, Mason JT, O'Leary TJ. 2007. A novel

HPLC method to resolve adenine species reveals depurination upon lithium

perchlorate/acetone precipitation, Analytical Biochemistry, 370:255-257.

14. Runstadler JA, Happ G, Slemons R, Sheng ZM, Gundlach N, Petrula M,

Senne D, Nolting J, Evers DL, Modrell A, Huson H, Hills S, Rothe T, Marr T,

Taubenberger JK. 2007. Using RRT-PCR analysis and virus isolation to

determine the prevalence of avian influenza virus infections in ducks at

Minto Flats State Game Refuge, Alaska, during August 2005. Archives of

Virology, 152:1901-1910.

13. Zhang Z, Evers DL, Dantonel J-C, Huong S-M, Huang E-S. 2006. Evidence

that the human cytomegalovirus IE2-86 protein binds mdm2 and facilitates

mdm2 degradation. Journal of Virology, 80:3833-3843.

12. Evers DL, Chao C-F, Wang X, Zhang Z, Huong S-M, Huang E-S. 2005. Human

cytomegalovirus-inhibitory flavonoids: studies on antiviral activity and

mechanism of action. Antiviral Research, 68:124-134.

11. Evers DL, Wang X, Huong S-M, Andreoni KA, Huang E-S. 2005. Inhibition

of human cytomegalovirus signaling and replication by the immunosuppressant

FK778. Antiviral Research, 65:1-12.

10. Evers DL, Komazin G, Ptak RG, Shin D, Emmer BT, Townsend LB, Drach JC.

2004. Inhibition of human cytomegalovirus replication by benzimidazole

nucleosides involves three distinct mechanisms. Antimicrobial Agents and

Chemotherapy, 48:3918-3927.

9. Evers DL, Wang X, Huang E-S. 2004. Cellular stress and signal

transduction responses to human cytomegalovirus infection. Microbes and

Infection, 6:1084-1093.

8. Evers DL, Wang X, Huong S-M, Huang DY, Huang E-S. 2004. 3,4',5-

trihydroxy-trans-stilbene (resveratrol) inhibits human cytomegalovirus

replication and virus-induced cellular signaling. Antiviral Research, 63:85-

95.

7. Evers DL, Komazin G, Shin D, Hwang DD, Townsend LB, Drach JC. 2002.

Interactions among antiviral drugs acting late in the replication cycle of

human cytomegalovirus. Antiviral Research, 56:61-72.

X. Evers DL. 2002. Early acting pyrrolo[2,3-d]pyrimidine and benzimidazole

inhibitors of human cytomegalovirus. University of Michigan Ph.D. Thesis.

6. Evers DL, Breitenbach JM, Borysko KZ, Townsend LB, Drach JC. 2002.

Inhibition of cyclin-dependent kinase 1 by purines and pyrrolo[2,3-

d]pyrimidines does not correlate with antiviral activity. Antimicrobial

Agents and Chemotherapy, 46:2470-2476.

5. Evers DL, Rice KG. "Mammalian Carbohydrate-Lectin Interactions" 2001. in

Glycoscience: Chemistry and Chemical Biology II. Eds. Fraser-Reid B,

Tatsuta K, Theim J. Springer-Verlag. pp. 1779-1816.

4. Collard WT, Evers DL, McKenzie DL, Rice KG. 2000. Synthesis of

homogeneous glycopeptides and their utility as DNA condensing agents.

Carbohydrate Research, 323:176-184.

3. Mo H, Rice KG, Evers DL, Winter HC, Peumans WJ, Van Damme EJM, Goldstein

IJ. 1999. Xanthosoma sagittifolium tubers contain a lectin with two

different types of carbohydrate-binding sites. Journal of Biological

Chemistry, 274:333**-*****.

2. Kwok KY, McKenzie DL, Evers DL, Rice KG. 1999. Formulation of highly

soluble poly(ethylene glycol)-peptide DNA condensates. Journal of

Pharmaceutical Sciences, 88:996-1003.

1. Evers DL, Hung RL, Thomas VH, Rice KG. 1998. Preparative purification of

a high-mannose type N-glycan from soy bean agglutinin by hydrazinolysis and

tyrosinamide derivatization. Analytical Biochemistry, 265:313-316.

Presentations/Additional Information

. Delivered talks at international meetings and less formal settings.

Comfortable preparing presentations and speaking in front of audiences

. Strong written communications skills learned from writing technical

reports, journal articles, research proposals, communications, patent

disclosures, budgets, and grants.

. Proficient with software such as Microsoft Office suite, Excel,

Sequencher, Adobe, as well as those designed for vector design,

database applications, and instrument-specific.

. Experience maintaining a laboratory by ordering supplies, organizing &

performing tasks, working with budgets, and maintaining a variety of

scientific equipment.

. Ad hoc reviewer for Avian Diseases and the Journal of Molecular

Diagnosis.

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