Srivatsan Gopalakrishnan

* ***** ******, ***#*, Brighton, MA, 02135 Phone # 617-***-****

Email: ab84hj@r.postjobfree.com

SUMMARY

Experienced Scientist/Engineer in Bio-Pharmaceutical industry. Have more

than 3 years' experience in cell culture process development, process -

tech transfer & process improvement, commercial GMP manufacturing

troubleshooting & root cause investigation. Good team player & always meet

project goals & deadlines.

My area of interest is process development, cell culture, project

management, bio-research, clinical research, regulatory affairs and

entrepreneurship in biotech and pharmaceutical industry.

SKILLS

Bioprocess skills:

. Commercial GMP manufacturing troubleshooting & root cause investigation

. Expertise in cell culture process development, process scale up and

process tech transfer from small scale to pilot scale and to

manufacturing

. Proficient in handling and operating of all types of bench scale

bioreactors, controllers, wave cultibags, and knowledge of use of DOE.

. Expertise with all the cell culture techniques, handling and maintaining

of mammalian cell lines.

. Experience in handling development, tech transfer projects and writing

reports

Bio analytical skills:

. HPLC, UPLC, Ion exchange chromatography, TLC, Electrophoresis, Ultra

centrifugation

Diagnostics skills:

. Expertise with immuno-detection techniques like ELISA, automated ELISA

instrument, SDS PAGE, Western blot

Computer & Statistical skills:

. Knowledge about Umetrics SIMCA P+

. Basic computer knowledge of MS Office and web applications, HTML.

General skills:

Strong interpersonal, organizational and communication skills & Strong

drives to meet project timelines.

WORK EXPERIENCE

Biogen Idec, Cambridge, MA

Associate Scientist, Manufacturing Sciences

May 2012 - present

. Performed Manufacturing Support, Investigations and troubleshooting

for upstream cell culture

. Participated in investigations of manufacturing root cause

deviations, analyzing and interpret the data

. Designed & performed small scale satellite runs and analyzed the

results

. Worked on small scale model development and process improvements

. Worked on project involving Virus inactivation in upstream cell

culture media and solutions

Biogen Idec, Cambridge, MA

Associate Scientist, Cell Culture Development

Jun 2009 - April 2012

. Worked extensively in developing process scale down and process

transfer for different cell culture processes.

. Handled and conducted experiments for technology and process transfer

from small scale to pilot scale and to manufacturing

. Used Wave reactors and cultibags for cell banking process.

. Worked extensively on Dasgip offline gas analyzer to monitor

physiological process during cell culture as part of technology

investment project and successfully transferred the technology from

small scale to pilot scale.

. Worked on technique to feed reactors based on OTR and RQ

. Worked on media development and media stability studies

. Experience writing reports for scale down model for process transfer,

department presentation

EMD Serono Research Center, Billerica, MA

Co-op, Phage Technology

Jun 2008-Dec 2008

. Carried out Antibody development and high throughput screening and

binding of antibodies.

. Expertise in large and small scale preparations of Fabs and test their

binding and efficacy using ELISA, automated ELISA and SDS PAGE.

. Also carried out DNA/RNA extractions, q-PCR techniques and western

blotting & handled bacterial cell cultures

. Routine inventory of chemicals and also assist in basic set up of lab.

Pfizer Ltd, Mumbai, India

Management trainee, Quality Operations department

Apr 2006 - July 2006

. Analyzed and handled raw materials and finished products to ensure their

quality using different chromatographic methods & Dissolution and

Disintegration chambers to test tablets and capsules

Screened and tested various raw materials used for manufacturing by

performing limit tests and other standard

test protocols, DMPK assays for

each raw material as under FDA guidance.

Molecular Diagnostics and Research Center, Thane, India

Summer internship

Apr 2002 - May 2002

. Gained hands on experience on various diagnostic tools and techniques,

ELISA, flow cytometry.

RESEARCH

Northeastern University:

. Center for Dug Discovery Research Guide:

Prof. Alexandros Makriyannis

Project: Analysis and evaluation potential selective inhibitor of MGL

and FAAH using radioligand and cAMP assays.

. New England Inflammation & Tissue Protection Institute

Research Guide: Prof. Michail Sitkvosky

Worked on DNA isolation and characterization, small animal handling,

ELISA, PCR, cell counter techniques.

Prepared and analyzed single cell suspensions using Fluorescent

Activated Cell Sorter (FACS).

Mumbai University:

. Institiute of Science

Research Guide: Prof. M.M Bapat

Project: Isolation and Characterization of Cellulose Degrading

Organisms

Isolated microorganisms from soil samples from nearby gas station

using different microbiological techniques.

Checked the cellulose degrading capability for microorganisms,

isolated and characterized them by various microbiological tests.

EDUCATION

Northeastern University, Boston, MA (Bouve College of Pharmacy and Health

Sciences)

Master of Sciences in Biotechnology, Specialization in Pharmaceutical

Biotechnology Jan 2009

Institute Of Science (University of Mumbai), Mumbai. India

Master of Science in Biochemistry

March 2005

Ramnarain Ruia College (University of Mumbai), Mumbai, India

Bachelor of Science in Biochemistry

May 2003

PRESENTATIONS, HONORS AND ACTIVITIES

. Attended the 15th annual Planova Bioprocess workshop 2012 in Boston

. Participated in the international symposia on 'Stem Cells and

Regenerative Medicines' held at Dhirubai Ambani Life Sciences.

. Awarded 2nd prize in chemistry model preparation in intracollege

competition at graduate level.

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